(Linear) (Six-base) MAPSORT of: KIEE5960.seq  Check: 1671  from: 1  to: 936



KIEE5960.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:16  ..

ApoI r'AATT_y

Cuts at:      0      31     936 
   Size:         31     905
 
BanII G_rGCy'C

Cuts at:      0     412     628     936 
   Size:        412     216     308

  Fragments arranged by size: 

                 412     308     216
 
BfrBI ATG'CAT

Cuts at:      0     826     936 
   Size:        826     110
 
BglII A'GATC_T

Cuts at:      0     299     936 
   Size:        299     637
 
Bme1580I G_kGCm'C

Cuts at:      0     328     936 
   Size:        328     608
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     936 
   Size:        604     332
 
BsaBI GATnn'nnATC

Cuts at:      0     158     806     936 
   Size:        158     648     130

  Fragments arranged by size: 

                 648     158     130
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     541     909     936 
   Size:        541     368      27

  Fragments arranged by size: 

                 541     368      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     924     936 
   Size:        148     776      12

  Fragments arranged by size: 

                 776     148      12
 
Bsp1286I G_dGCh'C

Cuts at:      0     328     412     628     936 
   Size:        328      84     216     308

  Fragments arranged by size: 

                 328     308     216      84
 
BspHI T'CATG_A

Cuts at:      0     404     936 
   Size:        404     532
 
BsrDI GCAATG_nn'

Cuts at:      0     896     936 
   Size:        896      40
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     319     936 
   Size:        319     617
 
BstYI r'GATC_y

Cuts at:      0     299     936 
   Size:        299     637
 
BtgI C'CryG_G

Cuts at:      0     149     350     936 
   Size:        149     201     586

  Fragments arranged by size: 

                 586     201     149
 
DraIII CAC_nnn'GTG

Cuts at:      0      21     936 
   Size:         21     915
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     905     936 
   Size:        905      31
 
EaeI y'GGCC_r

Cuts at:      0     371     936 
   Size:        371     565
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     936 
   Size:        604     332
 
EcoO109I rG'GnC_Cy

Cuts at:      0     705     936 
   Size:        705     231
 
FspI TGC'GCA

Cuts at:      0     917     936 
   Size:        917      19
 
MscI TGG'CCA

Cuts at:      0     373     936 
   Size:        373     563
 
MslI CAynn'nnrTG

Cuts at:      0     208     331     349     619     883     936 
   Size:        208     123      18     270     264      53

  Fragments arranged by size: 

                 270     264     208     123      53      18
 
NcoI C'CATG_G

Cuts at:      0     149     350     936 
   Size:        149     201     586

  Fragments arranged by size: 

                 586     201     149
 
NdeI CA'TA_TG

Cuts at:      0     376     936 
   Size:        376     560
 
NsiI A_TGCA'T

Cuts at:      0     828     936 
   Size:        828     108
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     468     582     936 
   Size:        468     114     354

  Fragments arranged by size: 

                 468     354     114
 
StyI C'CwwG_G

Cuts at:      0     149     350     701     936 
   Size:        149     201     351     235

  Fragments arranged by size: 

                 351     235     201     149
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     589     936 
   Size:        589     347
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     795     936 
   Size:        795     141
 

 Enzymes that do cut:

     ApoI    BanII    BfrBI    BglII Bme1580I     BpmI    BsaBI    BseRI
     BsgI Bsp1286I    BspHI    BsrDI    BstXI    BstYI     BtgI   DraIII
     DrdI     EaeI  Eco57MI EcoO109I     FspI     MscI     MslI     NcoI
     NdeI     NsiI    PflMI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI