Restriction map for
pFN21AE5959
(Linear) (Six-base) MAPSORT of: KIEE5959.seq Check: 7921 from: 1 to: 936
KIEE5959.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 936
Size: 727 209
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 78 936
Size: 36 42 858
Fragments arranged by size:
858 42 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 351 456 936
Size: 351 105 480
Fragments arranged by size:
480 351 105
AlwNI CAG_nnn'CTG
Cuts at: 0 21 936
Size: 21 915
BanI G'GyrC_C
Cuts at: 0 292 936
Size: 292 644
BanII G_rGCy'C
Cuts at: 0 457 936
Size: 457 479
BglI GCCn_nnn'nGGC
Cuts at: 0 352 936
Size: 352 584
BglII A'GATC_T
Cuts at: 0 59 936
Size: 59 877
BlpI GC'TnA_GC
Cuts at: 0 913 936
Size: 913 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 936
Size: 139 30 767
Fragments arranged by size:
767 139 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 147 415 714 936
Size: 147 268 299 222
Fragments arranged by size:
299 268 222 147
BseYI C'CCAG_C
Cuts at: 0 784 936
Size: 784 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 936
Size: 347 589
BsiHKAI G_wGCw'C
Cuts at: 0 404 936
Size: 404 532
Bsp1286I G_dGCh'C
Cuts at: 0 404 457 936
Size: 404 53 479
Fragments arranged by size:
479 404 53
BspHI T'CATG_A
Cuts at: 0 404 936
Size: 404 532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 320 727 936
Size: 320 407 209
Fragments arranged by size:
407 320 209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 936
Size: 243 693
BstYI r'GATC_y
Cuts at: 0 59 508 936
Size: 59 449 428
Fragments arranged by size:
449 428 59
BtgI C'CryG_G
Cuts at: 0 350 936
Size: 350 586
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 468 936
Size: 468 468
EaeI y'GGCC_r
Cuts at: 0 8 72 257 936
Size: 8 64 185 679
Fragments arranged by size:
679 185 64 8
EarI CTCTTCn'nnn_
Cuts at: 0 754 936
Size: 754 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 78 936
Size: 36 42 858
Fragments arranged by size:
858 42 36
EcoO109I rG'GnC_Cy
Cuts at: 0 540 936
Size: 540 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 802 834 936
Size: 802 32 102
Fragments arranged by size:
802 102 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 936
Size: 679 257
MscI TGG'CCA
Cuts at: 0 10 259 936
Size: 10 249 677
Fragments arranged by size:
677 249 10
MslI CAynn'nnrTG
Cuts at: 0 298 487 643 736 936
Size: 298 189 156 93 200
Fragments arranged by size:
298 200 189 156 93
MspA1I CmG'CkG
Cuts at: 0 255 936
Size: 255 681
NcoI C'CATG_G
Cuts at: 0 350 936
Size: 350 586
NspI r_CATG'y
Cuts at: 0 780 936
Size: 780 156
PasI CC'CwG_GG
Cuts at: 0 21 936
Size: 21 915
PflMI CCAn_nnn'nTGG
Cuts at: 0 21 936
Size: 21 915
PpuMI rG'GwC_Cy
Cuts at: 0 540 936
Size: 540 396
PsiI TTA'TAA
Cuts at: 0 187 936
Size: 187 749
PstI C_TGCA'G
Cuts at: 0 317 508 936
Size: 317 191 428
Fragments arranged by size:
428 317 191
PvuII CAG'CTG
Cuts at: 0 255 936
Size: 255 681
SfcI C'TryA_G
Cuts at: 0 313 504 936
Size: 313 191 432
Fragments arranged by size:
432 313 191
SspI AAT'ATT
Cuts at: 0 126 936
Size: 126 810
StuI AGG'CCT
Cuts at: 0 709 936
Size: 709 227
StyI C'CwwG_G
Cuts at: 0 282 350 936
Size: 282 68 586
Fragments arranged by size:
586 282 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 13 267 275 310 936
Size: 13 254 8 35 626
Fragments arranged by size:
626 254 35 13 8
TatI w'GTAC_w
Cuts at: 0 176 302 596 936
Size: 176 126 294 340
Fragments arranged by size:
340 294 176 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 936
Size: 139 32 765
Fragments arranged by size:
765 139 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 22 936
Size: 18 4 914
Fragments arranged by size:
914 18 4
Enzymes that do cut:
AarI AcuI AlfI AlwNI BanI BanII BglI BglII
BlpI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BstXI BstYI BtgI BtgZI EaeI EarI Eco57MI
EcoO109I Hin4I MmeI MscI MslI MspA1I NcoI NspI
PasI PflMI PpuMI PsiI PstI PvuII SfcI SspI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI