(Linear) (Six-base) MAPSORT of: KIEE5958.seq  Check: 687  from: 1  to: 936



KIEE5958.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:16  ..

AccI GT'mk_AC

Cuts at:      0     491     936 
   Size:        491     445
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671     936 
   Size:        671     265
 
AleI CACnn'nnGTG

Cuts at:      0     417     936 
   Size:        417     519
 
ApoI r'AATT_y

Cuts at:      0     805     936 
   Size:        805     131
 
AseI AT'TA_AT

Cuts at:      0     760     936 
   Size:        760     176
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     903     936 
   Size:        903      33
 
BglII A'GATC_T

Cuts at:      0     299     936 
   Size:        299     637
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      30     565     936 
   Size:         30     535     371

  Fragments arranged by size: 

                 535     371      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64      67     118     936 
   Size:         64       3      51     818

  Fragments arranged by size: 

                 818      64      51       3
 
BspHI T'CATG_A

Cuts at:      0     404     936 
   Size:        404     532
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     503     936 
   Size:        503     433
 
BsrDI GCAATG_nn'

Cuts at:      0     896     936 
   Size:        896      40
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     319     936 
   Size:        319     617
 
BstYI r'GATC_y

Cuts at:      0      65     299     936 
   Size:         65     234     637

  Fragments arranged by size: 

                 637     234      65
 
BtgI C'CryG_G

Cuts at:      0     350     936 
   Size:        350     586
 
DraIII CAC_nnn'GTG

Cuts at:      0      21     468     936 
   Size:         21     447     468

  Fragments arranged by size: 

                 468     447      21
 
EaeI y'GGCC_r

Cuts at:      0     188     371     936 
   Size:        188     183     565

  Fragments arranged by size: 

                 565     188     183
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     936 
   Size:         85     851
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671     936 
   Size:        671     265
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     108     140     936 
   Size:        108      32     796

  Fragments arranged by size: 

                 796     108      32
 
MscI TGG'CCA

Cuts at:      0     190     373     936 
   Size:        190     183     563

  Fragments arranged by size: 

                 563     190     183
 
MslI CAynn'nnrTG

Cuts at:      0     349     417     736     936 
   Size:        349      68     319     200

  Fragments arranged by size: 

                 349     319     200      68
 
NcoI C'CATG_G

Cuts at:      0     350     936 
   Size:        350     586
 
NdeI CA'TA_TG

Cuts at:      0     376     936 
   Size:        376     560
 
PfoI T'CCnGG_A

Cuts at:      0     442     936 
   Size:        442     494
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      22      54     936 
   Size:         22      32     882

  Fragments arranged by size: 

                 882      32      22
 
StyI C'CwwG_G

Cuts at:      0     350     936 
   Size:        350     586
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     377     936 
   Size:        377     559
 
TatI w'GTAC_w

Cuts at:      0     176     936 
   Size:        176     760
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      18     207     936 
   Size:         18     189     729

  Fragments arranged by size: 

                 729     189      18
 

 Enzymes that do cut:

     AccI     AcuI     AleI     ApoI     AseI     BbsI    BglII     BsaI
    BseRI    BspHI    BspMI    BsrDI    BstXI    BstYI     BtgI   DraIII
     EaeI     EarI  Eco57MI    Hin4I     MscI     MslI     NcoI     NdeI
     PfoI     PpiI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI