Restriction map for
pFN21AE5951
(Linear) (Six-base) MAPSORT of: KIEE5951.seq Check: 9953 from: 1 to: 936
KIEE5951.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 789 936
Size: 721 68 147
Fragments arranged by size:
721 147 68
AclI AA'CG_TT
Cuts at: 0 509 936
Size: 509 427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 568 936
Size: 555 13 368
Fragments arranged by size:
555 368 13
AlwNI CAG_nnn'CTG
Cuts at: 0 807 936
Size: 807 129
ApoI r'AATT_y
Cuts at: 0 25 914 936
Size: 25 889 22
Fragments arranged by size:
889 25 22
AvaI C'yCGr_G
Cuts at: 0 670 936
Size: 670 266
BbsI GAAGACnn'nnnn_
Cuts at: 0 288 735 936
Size: 288 447 201
Fragments arranged by size:
447 288 201
Bpu10I CC'TnA_GC
Cuts at: 0 290 936
Size: 290 646
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 936
Size: 316 30 590
Fragments arranged by size:
590 316 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 698 936
Size: 22 676 238
Fragments arranged by size:
676 238 22
BsiEI CG_ry'CG
Cuts at: 0 665 936
Size: 665 271
BsiHKAI G_wGCw'C
Cuts at: 0 671 936
Size: 671 265
Bsp1286I G_dGCh'C
Cuts at: 0 671 936
Size: 671 265
BspHI T'CATG_A
Cuts at: 0 35 351 584 905 936
Size: 35 316 233 321 31
Fragments arranged by size:
321 316 233 35 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 789 798 936
Size: 721 68 9 138
Fragments arranged by size:
721 138 68 9
BsrDI GCAATG_nn'
Cuts at: 0 582 936
Size: 582 354
BssSI C'ACGA_G
Cuts at: 0 301 936
Size: 301 635
BstXI CCAn_nnnn'nTGG
Cuts at: 0 21 936
Size: 21 915
BstYI r'GATC_y
Cuts at: 0 804 936
Size: 804 132
Bsu36I CC'TnA_GG
Cuts at: 0 181 772 936
Size: 181 591 164
Fragments arranged by size:
591 181 164
EaeI y'GGCC_r
Cuts at: 0 890 936
Size: 890 46
EarI CTCTTCn'nnn_
Cuts at: 0 765 904 936
Size: 765 139 32
Fragments arranged by size:
765 139 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 568 936
Size: 555 13 368
Fragments arranged by size:
555 368 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 936
Size: 523 32 381
Fragments arranged by size:
523 381 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 936
Size: 530 406
MscI TGG'CCA
Cuts at: 0 892 936
Size: 892 44
MslI CAynn'nnrTG
Cuts at: 0 19 166 343 421 730 936
Size: 19 147 177 78 309 206
Fragments arranged by size:
309 206 177 147 78 19
MspA1I CmG'CkG
Cuts at: 0 489 936
Size: 489 447
NspI r_CATG'y
Cuts at: 0 165 468 936
Size: 165 303 468
Fragments arranged by size:
468 303 165
PstI C_TGCA'G
Cuts at: 0 795 936
Size: 795 141
PvuII CAG'CTG
Cuts at: 0 489 936
Size: 489 447
SapI GCTCTTCn'nnn_
Cuts at: 0 765 936
Size: 765 171
SfcI C'TryA_G
Cuts at: 0 61 791 936
Size: 61 730 145
Fragments arranged by size:
730 145 61
SphI G_CATG'C
Cuts at: 0 468 936
Size: 468 468
StuI AGG'CCT
Cuts at: 0 703 936
Size: 703 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 491 814 936
Size: 491 323 122
Fragments arranged by size:
491 323 122
TatI w'GTAC_w
Cuts at: 0 170 936
Size: 170 766
Enzymes that do cut:
AarI AclI AcuI AlwNI ApoI AvaI BbsI Bpu10I
BsaXI BseRI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BssSI BstXI BstYI Bsu36I EaeI EarI Eco57MI Hin4I
MmeI MscI MslI MspA1I NspI PstI PvuII SapI
SfcI SphI StuI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiWI BsmI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StyI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI