Restriction map for
pFN21AE5948
(Linear) (Six-base) MAPSORT of: KIEE5948.seq Check: 1273 from: 1 to: 936
KIEE5948.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 503 936
Size: 503 433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 936
Size: 661 275
AflII C'TTAA_G
Cuts at: 0 777 936
Size: 777 159
AhdI GACnn_n'nnGTC
Cuts at: 0 255 936
Size: 255 681
AlwNI CAG_nnn'CTG
Cuts at: 0 75 936
Size: 75 861
ApaI G_GGCC'C
Cuts at: 0 162 936
Size: 162 774
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 707 740 936
Size: 707 33 196
Fragments arranged by size:
707 196 33
BanI G'GyrC_C
Cuts at: 0 118 936
Size: 118 818
BanII G_rGCy'C
Cuts at: 0 162 936
Size: 162 774
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 936
Size: 274 662
BciVI GTATCCnnnnn_n'
Cuts at: 0 837 936
Size: 837 99
Bme1580I G_kGCm'C
Cuts at: 0 121 162 936
Size: 121 41 774
Fragments arranged by size:
774 121 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 703 936
Size: 703 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 786 894 936
Size: 538 248 108 42
Fragments arranged by size:
538 248 108 42
BsaBI GATnn'nnATC
Cuts at: 0 592 936
Size: 592 344
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 789 936
Size: 88 701 147
Fragments arranged by size:
701 147 88
Bsp1286I G_dGCh'C
Cuts at: 0 121 162 936
Size: 121 41 774
Fragments arranged by size:
774 121 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 503 936
Size: 260 243 433
Fragments arranged by size:
433 260 243
BsrBI CCG'CTC
Cuts at: 0 6 936
Size: 6 930
BsrDI GCAATG_nn'
Cuts at: 0 539 936
Size: 539 397
BsrGI T'GTAC_A
Cuts at: 0 607 936
Size: 607 329
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 936
Size: 75 861
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 243 936
Size: 150 93 693
Fragments arranged by size:
693 150 93
BtsI GCAGTG_nn'
Cuts at: 0 385 936
Size: 385 551
EaeI y'GGCC_r
Cuts at: 0 59 936
Size: 59 877
EarI CTCTTCn'nnn_
Cuts at: 0 319 936
Size: 319 617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 936
Size: 661 275
EcoO109I rG'GnC_Cy
Cuts at: 0 158 159 936
Size: 158 1 777
Fragments arranged by size:
777 158 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 241 273 877 909 936
Size: 20 32 189 32 604 32 27
Fragments arranged by size:
604 189 32 32 32 27 20
HincII GTy'rAC
Cuts at: 0 249 936
Size: 249 687
HindIII A'AGCT_T
Cuts at: 0 707 936
Size: 707 229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 936
Size: 815 121
MscI TGG'CCA
Cuts at: 0 61 936
Size: 61 875
MslI CAynn'nnrTG
Cuts at: 0 298 598 936
Size: 298 300 338
Fragments arranged by size:
338 300 298
MspA1I CmG'CkG
Cuts at: 0 75 936
Size: 75 861
PfoI T'CCnGG_A
Cuts at: 0 149 936
Size: 149 787
PsiI TTA'TAA
Cuts at: 0 841 936
Size: 841 95
PspOMI G'GGCC_C
Cuts at: 0 158 936
Size: 158 778
PstI C_TGCA'G
Cuts at: 0 71 936
Size: 71 865
PvuII CAG'CTG
Cuts at: 0 75 936
Size: 75 861
SfcI C'TryA_G
Cuts at: 0 67 936
Size: 67 869
SmlI C'TyrA_G
Cuts at: 0 553 777 801 873 936
Size: 553 224 24 72 63
Fragments arranged by size:
553 224 72 63 24
SpeI A'CTAG_T
Cuts at: 0 429 936
Size: 429 507
StuI AGG'CCT
Cuts at: 0 783 936
Size: 783 153
StyI C'CwwG_G
Cuts at: 0 318 482 936
Size: 318 164 454
Fragments arranged by size:
454 318 164
TatI w'GTAC_w
Cuts at: 0 176 607 936
Size: 176 431 329
Fragments arranged by size:
431 329 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 401 433 936
Size: 401 32 503
Fragments arranged by size:
503 401 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 279 936
Size: 147 132 657
Fragments arranged by size:
657 147 132
XmnI GAAnn'nnTTC
Cuts at: 0 924 936
Size: 924 12
Enzymes that do cut:
AarI AcuI AflII AhdI AlwNI ApaI BaeI BanI
BanII BbsI BciVI Bme1580I BmrI BpuEI BsaBI BseRI
Bsp1286I BspMI BsrBI BsrDI BsrGI BstAPI BstXI BtsI
EaeI EarI Eco57MI EcoO109I Hin4I HincII HindIII MmeI
MscI MslI MspA1I PfoI PsiI PspOMI PstI PvuII
SfcI SmlI SpeI StuI StyI TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrFI BssHII BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI SwaI TaqII Tth111I XbaI
XhoI XmaI ZraI