(Linear) (Six-base) MAPSORT of: KIEE5944.seq  Check: 2845  from: 1  to: 936



KIEE5944.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:15  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     429     936 
   Size:        429     507
 
ApoI r'AATT_y

Cuts at:      0      31     249     684     936 
   Size:         31     218     435     252

  Fragments arranged by size: 

                 435     252     218      31
 
BanI G'GyrC_C

Cuts at:      0      72     936 
   Size:         72     864
 
BanII G_rGCy'C

Cuts at:      0     726     936 
   Size:        726     210
 
BlpI GC'TnA_GC

Cuts at:      0      62     936 
   Size:         62     874
 
BmtI G_CTAG'C

Cuts at:      0     304     936 
   Size:        304     632
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     936 
   Size:        141     795
 
BsaAI yAC'GTr

Cuts at:      0     774     936 
   Size:        774     162
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     796     936 
   Size:        796     140
 
BsiEI CG_ry'CG

Cuts at:      0     363     936 
   Size:        363     573
 
Bsp1286I G_dGCh'C

Cuts at:      0     726     936 
   Size:        726     210
 
BspHI T'CATG_A

Cuts at:      0      95     936 
   Size:         95     841
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     662     936 
   Size:        662     274
 
BstYI r'GATC_y

Cuts at:      0     207     936 
   Size:        207     729
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     209     244     936 
   Size:        209      35     692

  Fragments arranged by size: 

                 692     209      35
 
EaeI y'GGCC_r

Cuts at:      0     371     936 
   Size:        371     565
 
EarI CTCTTCn'nnn_

Cuts at:      0     473     635     936 
   Size:        473     162     301

  Fragments arranged by size: 

                 473     301     162
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     936 
   Size:        141     795
 
EcoO109I rG'GnC_Cy

Cuts at:      0     232     936 
   Size:        232     704
 
EcoRI G'AATT_C

Cuts at:      0      31     684     936 
   Size:         31     653     252

  Fragments arranged by size: 

                 653     252      31
 
EcoRV GAT'ATC

Cuts at:      0      57     936 
   Size:         57     879
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     612     644     936 
   Size:        612      32     292

  Fragments arranged by size: 

                 612     292      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     936 
   Size:        131     805
 
MscI TGG'CCA

Cuts at:      0     373     936 
   Size:        373     563
 
MslI CAynn'nnrTG

Cuts at:      0     772     936 
   Size:        772     164
 
NheI G'CTAG_C

Cuts at:      0     300     936 
   Size:        300     636
 
PpuMI rG'GwC_Cy

Cuts at:      0     232     936 
   Size:        232     704
 
PshAI GACnn'nnGTC

Cuts at:      0     736     936 
   Size:        736     200
 
PstI C_TGCA'G

Cuts at:      0      71     936 
   Size:         71     865
 
SanDI GG'GwC_CC

Cuts at:      0     232     936 
   Size:        232     704
 
SfcI C'TryA_G

Cuts at:      0      67     864     936 
   Size:         67     797      72

  Fragments arranged by size: 

                 797      72      67
 
SspI AAT'ATT

Cuts at:      0     927     936 
   Size:        927       9
 
TatI w'GTAC_w

Cuts at:      0     176     936 
   Size:        176     760
 
XmnI GAAnn'nnTTC

Cuts at:      0      31      35     936 
   Size:         31       4     901

  Fragments arranged by size: 

                 901      31       4
 

 Enzymes that do cut:

    AlwNI     ApoI     BanI    BanII     BlpI     BmtI     BpmI    BsaAI
     BsgI    BsiEI Bsp1286I    BspHI    BspMI    BstYI    CspCI     EaeI
     EarI  Eco57MI EcoO109I    EcoRI    EcoRV    Hin4I     MmeI     MscI
     MslI     NheI    PpuMI    PshAI     PstI    SanDI     SfcI     SspI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI