Restriction map for
pFN21AE5940
(Linear) (Six-base) MAPSORT of: KIEE5940.seq Check: 1525 from: 1 to: 936
KIEE5940.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 779 811 936
Size: 779 32 125
Fragments arranged by size:
779 125 32
AvaI C'yCGr_G
Cuts at: 0 322 410 936
Size: 322 88 526
Fragments arranged by size:
526 322 88
BanI G'GyrC_C
Cuts at: 0 121 936
Size: 121 815
BanII G_rGCy'C
Cuts at: 0 167 936
Size: 167 769
BciVI GTATCCnnnnn_n'
Cuts at: 0 465 936
Size: 465 471
BfrBI ATG'CAT
Cuts at: 0 523 936
Size: 523 413
BglI GCCn_nnn'nGGC
Cuts at: 0 190 936
Size: 190 746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 936
Size: 666 270
Bpu10I CC'TnA_GC
Cuts at: 0 670 936
Size: 670 266
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 936
Size: 643 293
BsaBI GATnn'nnATC
Cuts at: 0 152 453 936
Size: 152 301 483
Fragments arranged by size:
483 301 152
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 703 936
Size: 469 234 233
Fragments arranged by size:
469 234 233
BseYI C'CCAG_C
Cuts at: 0 237 429 588 936
Size: 237 192 159 348
Fragments arranged by size:
348 237 192 159
BsmI GAATG_Cn'
Cuts at: 0 332 523 900 936
Size: 332 191 377 36
Fragments arranged by size:
377 332 191 36
Bsp1286I G_dGCh'C
Cuts at: 0 167 936
Size: 167 769
BspHI T'CATG_A
Cuts at: 0 159 936
Size: 159 777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 783 936
Size: 783 153
BsrDI GCAATG_nn'
Cuts at: 0 123 674 936
Size: 123 551 262
Fragments arranged by size:
551 262 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 204 237 928 936
Size: 204 33 691 8
Fragments arranged by size:
691 204 33 8
BtgI C'CryG_G
Cuts at: 0 230 344 936
Size: 230 114 592
Fragments arranged by size:
592 230 114
EaeI y'GGCC_r
Cuts at: 0 182 936
Size: 182 754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 936
Size: 666 270
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 936
Size: 535 401
MscI TGG'CCA
Cuts at: 0 184 936
Size: 184 752
MslI CAynn'nnrTG
Cuts at: 0 506 539 936
Size: 506 33 397
Fragments arranged by size:
506 397 33
MspA1I CmG'CkG
Cuts at: 0 11 592 936
Size: 11 581 344
Fragments arranged by size:
581 344 11
NcoI C'CATG_G
Cuts at: 0 344 936
Size: 344 592
NsiI A_TGCA'T
Cuts at: 0 525 936
Size: 525 411
NspI r_CATG'y
Cuts at: 0 523 936
Size: 523 413
PspXI vC'TCGA_Gb
Cuts at: 0 410 936
Size: 410 526
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 446 478 936
Size: 446 32 458
Fragments arranged by size:
458 446 32
PvuII CAG'CTG
Cuts at: 0 592 936
Size: 592 344
SmlI C'TyrA_G
Cuts at: 0 322 410 658 936
Size: 322 88 248 278
Fragments arranged by size:
322 278 248 88
StuI AGG'CCT
Cuts at: 0 295 936
Size: 295 641
StyI C'CwwG_G
Cuts at: 0 344 698 936
Size: 344 354 238
Fragments arranged by size:
354 344 238
TatI w'GTAC_w
Cuts at: 0 170 396 936
Size: 170 226 540
Fragments arranged by size:
540 226 170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 58 90 298 330 936
Size: 58 32 208 32 606
Fragments arranged by size:
606 208 58 32 32
XhoI C'TCGA_G
Cuts at: 0 322 410 936
Size: 322 88 526
Fragments arranged by size:
526 322 88
Enzymes that do cut:
AloI AvaI BanI BanII BciVI BfrBI BglI BpmI
Bpu10I BpuEI BsaBI BseRI BseYI BsmI Bsp1286I BspHI
BspMI BsrDI BstXI BtgI EaeI Eco57MI EcoNI MscI
MslI MspA1I NcoI NsiI NspI PspXI PsrI PvuII
SmlI StuI StyI TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BclI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BsaI BsaAI BsaHI BsaWI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XcmI XmaI XmnI ZraI