(Linear) (Six-base) MAPSORT of: KIEE5939.seq  Check: 3212  from: 1  to: 933



KIEE5939.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:15  ..

AclI AA'CG_TT

Cuts at:      0     512     933 
   Size:        512     421
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     806     933 
   Size:         88     718     127

  Fragments arranged by size: 

                 718     127      88
 
AlwNI CAG_nnn'CTG

Cuts at:      0     480     933 
   Size:        480     453
 
ApoI r'AATT_y

Cuts at:      0      31     933 
   Size:         31     902
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     791     933 
   Size:        791     142
 
BglII A'GATC_T

Cuts at:      0     299     933 
   Size:        299     634
 
BsiEI CG_ry'CG

Cuts at:      0     363     933 
   Size:        363     570
 
BsiHKAI G_wGCw'C

Cuts at:      0     457     933 
   Size:        457     476
 
BsmI GAATG_Cn'

Cuts at:      0     686     933 
   Size:        686     247
 
Bsp1286I G_dGCh'C

Cuts at:      0     457     933 
   Size:        457     476
 
BspHI T'CATG_A

Cuts at:      0      41     404     449     933 
   Size:         41     363      45     484

  Fragments arranged by size: 

                 484     363      45      41
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     933 
   Size:        134     799
 
BsrGI T'GTAC_A

Cuts at:      0     773     830     933 
   Size:        773      57     103

  Fragments arranged by size: 

                 773     103      57
 
BstEII G'GTnAC_C

Cuts at:      0     108     933 
   Size:        108     825
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     105     933 
   Size:        105     828
 
BstYI r'GATC_y

Cuts at:      0     239     299     933 
   Size:        239      60     634

  Fragments arranged by size: 

                 634     239      60
 
EaeI y'GGCC_r

Cuts at:      0     371     933 
   Size:        371     562
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     806     933 
   Size:         88     718     127

  Fragments arranged by size: 

                 718     127      88
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4      57     933 
   Size:          4      53     876

  Fragments arranged by size: 

                 876      53       4
 
HindIII A'AGCT_T

Cuts at:      0     896     933 
   Size:        896      37
 
MscI TGG'CCA

Cuts at:      0     373     933 
   Size:        373     560
 
MslI CAynn'nnrTG

Cuts at:      0      46     172     448     933 
   Size:         46     126     276     485

  Fragments arranged by size: 

                 485     276     126      46
 
MspA1I CmG'CkG

Cuts at:      0      22     495     933 
   Size:         22     473     438

  Fragments arranged by size: 

                 473     438      22
 
PpuMI rG'GwC_Cy

Cuts at:      0       4      57     933 
   Size:          4      53     876

  Fragments arranged by size: 

                 876      53       4
 
PvuII CAG'CTG

Cuts at:      0      22     495     933 
   Size:         22     473     438

  Fragments arranged by size: 

                 473     438      22
 
SanDI GG'GwC_CC

Cuts at:      0       4     933 
   Size:          4     929
 
SexAI A'CCwGG_T

Cuts at:      0     104     933 
   Size:        104     829
 
SfcI C'TryA_G

Cuts at:      0     867     933 
   Size:        867      66
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     310     398     933 
   Size:        310      88     535

  Fragments arranged by size: 

                 535     310      88
 
TatI w'GTAC_w

Cuts at:      0     176     773     830     933 
   Size:        176     597      57     103

  Fragments arranged by size: 

                 597     176     103      57
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     542     859     933 
   Size:         35     507     317      74

  Fragments arranged by size: 

                 507     317      74      35
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI     BbsI    BglII    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrGI   BstEII    BstXI    BstYI
     EaeI  Eco57MI EcoO109I  HindIII     MscI     MslI   MspA1I    PpuMI
    PvuII    SanDI    SexAI     SfcI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI