Restriction map for
pFN21AE5937
(Linear) (Six-base) MAPSORT of: KIEE5937.seq Check: 4263 from: 1 to: 933
KIEE5937.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AfeI AGC'GCT
Cuts at: 0 59 933
Size: 59 874
AflIII A'CryG_T
Cuts at: 0 203 933
Size: 203 730
AvaI C'yCGr_G
Cuts at: 0 618 933
Size: 618 315
AvrII C'CTAG_G
Cuts at: 0 315 933
Size: 315 618
BamHI G'GATC_C
Cuts at: 0 515 677 933
Size: 515 162 256
Fragments arranged by size:
515 256 162
BanI G'GyrC_C
Cuts at: 0 225 322 933
Size: 225 97 611
Fragments arranged by size:
611 225 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 75 933
Size: 75 858
BbvCI CC'TCA_GC
Cuts at: 0 573 933
Size: 573 360
BclI T'GATC_A
Cuts at: 0 137 933
Size: 137 796
BlpI GC'TnA_GC
Cuts at: 0 293 933
Size: 293 640
Bme1580I G_kGCm'C
Cuts at: 0 230 325 933
Size: 230 95 608
Fragments arranged by size:
608 230 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 416 933
Size: 416 517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 933
Size: 771 162
Bpu10I CC'TnA_GC
Cuts at: 0 385 573 933
Size: 385 188 360
Fragments arranged by size:
385 360 188
BsaAI yAC'GTr
Cuts at: 0 99 933
Size: 99 834
BsaHI Gr'CG_yC
Cuts at: 0 65 835 933
Size: 65 770 98
Fragments arranged by size:
770 98 65
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 933
Size: 112 821
BseYI C'CCAG_C
Cuts at: 0 54 111 933
Size: 54 57 822
Fragments arranged by size:
822 57 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 584 933
Size: 442 142 349
Fragments arranged by size:
442 349 142
BsiHKAI G_wGCw'C
Cuts at: 0 104 933
Size: 104 829
Bsp1286I G_dGCh'C
Cuts at: 0 104 230 325 933
Size: 104 126 95 608
Fragments arranged by size:
608 126 104 95
BsrBI CCG'CTC
Cuts at: 0 384 933
Size: 384 549
BsrGI T'GTAC_A
Cuts at: 0 857 933
Size: 857 76
BssSI C'ACGA_G
Cuts at: 0 414 933
Size: 414 519
BstYI r'GATC_y
Cuts at: 0 515 677 933
Size: 515 162 256
Fragments arranged by size:
515 256 162
Bsu36I CC'TnA_GG
Cuts at: 0 775 865 933
Size: 775 90 68
Fragments arranged by size:
775 90 68
DraIII CAC_nnn'GTG
Cuts at: 0 329 933
Size: 329 604
EaeI y'GGCC_r
Cuts at: 0 368 428 812 933
Size: 368 60 384 121
Fragments arranged by size:
384 368 121 60
EarI CTCTTCn'nnn_
Cuts at: 0 428 933
Size: 428 505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 933
Size: 771 162
HaeII r_GCGC'y
Cuts at: 0 61 414 933
Size: 61 353 519
Fragments arranged by size:
519 353 61
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 585 933
Size: 29 556 348
Fragments arranged by size:
556 348 29
MscI TGG'CCA
Cuts at: 0 370 430 933
Size: 370 60 503
Fragments arranged by size:
503 370 60
MslI CAynn'nnrTG
Cuts at: 0 34 166 202 397 595 933
Size: 34 132 36 195 198 338
Fragments arranged by size:
338 198 195 132 36 34
MspA1I CmG'CkG
Cuts at: 0 284 933
Size: 284 649
NspI r_CATG'y
Cuts at: 0 207 933
Size: 207 726
PciI A'CATG_T
Cuts at: 0 203 933
Size: 203 730
PflMI CCAn_nnn'nTGG
Cuts at: 0 440 933
Size: 440 493
PfoI T'CCnGG_A
Cuts at: 0 787 933
Size: 787 146
PvuII CAG'CTG
Cuts at: 0 284 933
Size: 284 649
SfcI C'TryA_G
Cuts at: 0 339 933
Size: 339 594
StyI C'CwwG_G
Cuts at: 0 315 933
Size: 315 618
TatI w'GTAC_w
Cuts at: 0 170 418 857 933
Size: 170 248 439 76
Fragments arranged by size:
439 248 170 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 530 562 933
Size: 530 32 371
Fragments arranged by size:
530 371 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 552 795 933
Size: 552 243 138
Fragments arranged by size:
552 243 138
Enzymes that do cut:
AfeI AflIII AvaI AvrII BamHI BanI BbsI BbvCI
BclI BlpI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI
BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrGI BssSI
BstYI Bsu36I DraIII EaeI EarI Eco57MI HaeII MmeI
MscI MslI MspA1I NspI PciI PflMI PfoI PvuII
SfcI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BanII BbeI BcgI BciVI
BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaI
BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BssHII BstAPI BstBI BstEII
BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI