Restriction map for
pFN21AE5935
(Linear) (Six-base) MAPSORT of: KIEE5935.seq Check: 40 from: 1 to: 933
KIEE5935.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 933
Size: 749 184
AflIII A'CryG_T
Cuts at: 0 77 721 933
Size: 77 644 212
Fragments arranged by size:
644 212 77
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 152 166 184 198 933
Size: 152 14 18 14 735
Fragments arranged by size:
735 152 18 14 14
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 567 600 933
Size: 567 33 333
Fragments arranged by size:
567 333 33
BanII G_rGCy'C
Cuts at: 0 179 933
Size: 179 754
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 669 762 933
Size: 74 595 93 171
Fragments arranged by size:
595 171 93 74
BbvCI CC'TCA_GC
Cuts at: 0 341 933
Size: 341 592
BlpI GC'TnA_GC
Cuts at: 0 701 933
Size: 701 232
BmrI ACTGGGnnnn_n'
Cuts at: 0 25 933
Size: 25 908
Bpu10I CC'TnA_GC
Cuts at: 0 341 715 933
Size: 341 374 218
Fragments arranged by size:
374 341 218
BsaAI yAC'GTr
Cuts at: 0 722 933
Size: 722 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 152 166 182 196 281 311 933
Size: 152 14 16 14 85 30 622
Fragments arranged by size:
622 152 85 30 16 14 14
BseYI C'CCAG_C
Cuts at: 0 123 249 933
Size: 123 126 684
Fragments arranged by size:
684 126 123
BsiHKAI G_wGCw'C
Cuts at: 0 390 548 641 914 933
Size: 390 158 93 273 19
Fragments arranged by size:
390 273 158 93 19
Bsp1286I G_dGCh'C
Cuts at: 0 179 390 548 641 914 933
Size: 179 211 158 93 273 19
Fragments arranged by size:
273 211 179 158 93 19
BsrDI GCAATG_nn'
Cuts at: 0 135 554 933
Size: 135 419 379
Fragments arranged by size:
419 379 135
BssSI C'ACGA_G
Cuts at: 0 274 933
Size: 274 659
BstYI r'GATC_y
Cuts at: 0 83 933
Size: 83 850
BtgI C'CryG_G
Cuts at: 0 479 933
Size: 479 454
ClaI AT'CG_AT
Cuts at: 0 789 933
Size: 789 144
EaeI y'GGCC_r
Cuts at: 0 194 377 933
Size: 194 183 556
Fragments arranged by size:
556 194 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 670 933
Size: 670 263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 933
Size: 749 184
HaeII r_GCGC'y
Cuts at: 0 731 933
Size: 731 202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 717 933
Size: 717 216
MscI TGG'CCA
Cuts at: 0 196 933
Size: 196 737
MslI CAynn'nnrTG
Cuts at: 0 331 439 933
Size: 331 108 494
Fragments arranged by size:
494 331 108
NcoI C'CATG_G
Cuts at: 0 479 933
Size: 479 454
NspI r_CATG'y
Cuts at: 0 81 249 444 933
Size: 81 168 195 489
Fragments arranged by size:
489 195 168 81
PciI A'CATG_T
Cuts at: 0 77 933
Size: 77 856
PflMI CCAn_nnn'nTGG
Cuts at: 0 426 933
Size: 426 507
PfoI T'CCnGG_A
Cuts at: 0 170 933
Size: 170 763
PmlI CAC'GTG
Cuts at: 0 722 933
Size: 722 211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 152 166 184 198 896 928 933
Size: 152 14 18 14 698 32 5
Fragments arranged by size:
698 152 32 18 14 14 5
SexAI A'CCwGG_T
Cuts at: 0 632 807 933
Size: 632 175 126
Fragments arranged by size:
632 175 126
SphI G_CATG'C
Cuts at: 0 249 933
Size: 249 684
StyI C'CwwG_G
Cuts at: 0 479 933
Size: 479 454
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 44 933
Size: 44 889
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 281 313 933
Size: 281 32 620
Fragments arranged by size:
620 281 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 213 325 933
Size: 213 112 608
Fragments arranged by size:
608 213 112
Enzymes that do cut:
AcuI AflIII AloI BaeI BanII BbsI BbvCI BlpI
BmrI Bpu10I BsaAI BsaXI BseYI BsiHKAI Bsp1286I BsrDI
BssSI BstYI BtgI ClaI EaeI EciI Eco57MI HaeII
MmeI MscI MslI NcoI NspI PciI PflMI PfoI
PmlI PpiI SexAI SphI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BbeI BcgI
BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI