Restriction map for
pFN21AE5932
(Linear) (Six-base) MAPSORT of: KIEE5932.seq Check: 129 from: 1 to: 933
KIEE5932.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 404 933
Size: 404 529
AatII G_ACGT'C
Cuts at: 0 797 933
Size: 797 136
AflIII A'CryG_T
Cuts at: 0 203 933
Size: 203 730
AleI CACnn'nnGTG
Cuts at: 0 397 933
Size: 397 536
AvaI C'yCGr_G
Cuts at: 0 618 933
Size: 618 315
BamHI G'GATC_C
Cuts at: 0 677 933
Size: 677 256
BanI G'GyrC_C
Cuts at: 0 225 933
Size: 225 708
BbvCI CC'TCA_GC
Cuts at: 0 573 933
Size: 573 360
BclI T'GATC_A
Cuts at: 0 137 933
Size: 137 796
BglI GCCn_nnn'nGGC
Cuts at: 0 427 933
Size: 427 506
BlpI GC'TnA_GC
Cuts at: 0 293 933
Size: 293 640
Bme1580I G_kGCm'C
Cuts at: 0 230 423 933
Size: 230 193 510
Fragments arranged by size:
510 230 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 933
Size: 771 162
Bpu10I CC'TnA_GC
Cuts at: 0 385 573 933
Size: 385 188 360
Fragments arranged by size:
385 360 188
BsaI GGTCTCn'nnnn_
Cuts at: 0 771 933
Size: 771 162
BsaAI yAC'GTr
Cuts at: 0 99 933
Size: 99 834
BsaBI GATnn'nnATC
Cuts at: 0 589 933
Size: 589 344
BsaHI Gr'CG_yC
Cuts at: 0 65 794 933
Size: 65 729 139
Fragments arranged by size:
729 139 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 402 432 933
Size: 402 30 501
Fragments arranged by size:
501 402 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 933
Size: 112 821
BseYI C'CCAG_C
Cuts at: 0 111 933
Size: 111 822
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 584 933
Size: 442 142 349
Fragments arranged by size:
442 349 142
BsiHKAI G_wGCw'C
Cuts at: 0 104 325 933
Size: 104 221 608
Fragments arranged by size:
608 221 104
Bsp1286I G_dGCh'C
Cuts at: 0 104 230 325 423 933
Size: 104 126 95 98 510
Fragments arranged by size:
510 126 104 98 95
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 404 933
Size: 404 529
BsrBI CCG'CTC
Cuts at: 0 384 933
Size: 384 549
BsrGI T'GTAC_A
Cuts at: 0 857 933
Size: 857 76
BssSI C'ACGA_G
Cuts at: 0 16 933
Size: 16 917
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 933
Size: 585 348
BstYI r'GATC_y
Cuts at: 0 515 677 933
Size: 515 162 256
Fragments arranged by size:
515 256 162
BtsI GCAGTG_nn'
Cuts at: 0 726 933
Size: 726 207
DraIII CAC_nnn'GTG
Cuts at: 0 329 933
Size: 329 604
DrdI GACnn_nn'nnGTC
Cuts at: 0 249 933
Size: 249 684
EaeI y'GGCC_r
Cuts at: 0 368 428 812 933
Size: 368 60 384 121
Fragments arranged by size:
384 368 121 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 933
Size: 771 162
MscI TGG'CCA
Cuts at: 0 370 430 814 933
Size: 370 60 384 119
Fragments arranged by size:
384 370 119 60
MslI CAynn'nnrTG
Cuts at: 0 166 202 346 397 595 933
Size: 166 36 144 51 198 338
Fragments arranged by size:
338 198 166 144 51 36
MspA1I CmG'CkG
Cuts at: 0 284 933
Size: 284 649
NspI r_CATG'y
Cuts at: 0 207 933
Size: 207 726
PasI CC'CwG_GG
Cuts at: 0 55 933
Size: 55 878
PciI A'CATG_T
Cuts at: 0 203 933
Size: 203 730
PflMI CCAn_nnn'nTGG
Cuts at: 0 76 315 440 933
Size: 76 239 125 493
Fragments arranged by size:
493 239 125 76
PvuII CAG'CTG
Cuts at: 0 284 933
Size: 284 649
StyI C'CwwG_G
Cuts at: 0 245 933
Size: 245 688
TatI w'GTAC_w
Cuts at: 0 170 857 933
Size: 170 687 76
Fragments arranged by size:
687 170 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 59 402 434 530 562 933
Size: 27 32 343 32 96 32 371
Fragments arranged by size:
371 343 96 32 32 32 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 552 795 933
Size: 552 243 138
Fragments arranged by size:
552 243 138
ZraI GAC'GTC
Cuts at: 0 795 933
Size: 795 138
Enzymes that do cut:
AarI AatII AflIII AleI AvaI BamHI BanI BbvCI
BclI BglI BlpI Bme1580I BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrGI BssSI BstXI BstYI BtsI DraIII
DrdI EaeI Eco57MI MscI MslI MspA1I NspI PasI
PciI PflMI PvuII StyI TatI TstI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanII BbeI BbsI BcgI BciVI
BfrBI BglII BmgBI BmrI BmtI BplI BpuEI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI EagI EarI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI