(Linear) (Six-base) MAPSORT of: KIEE5930.seq  Check: 4248  from: 1  to: 933



KIEE5930.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      54     721     933 
   Size:         54     667     212

  Fragments arranged by size: 

                 667     212      54
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     665     933 
   Size:        202     463     268

  Fragments arranged by size: 

                 463     268     202
 
AfeI AGC'GCT

Cuts at:      0     107     933 
   Size:        107     826
 
AflIII A'CryG_T

Cuts at:      0     479     933 
   Size:        479     454
 
AleI CACnn'nnGTG

Cuts at:      0      16     933 
   Size:         16     917
 
AlwNI CAG_nnn'CTG

Cuts at:      0      69     223     933 
   Size:         69     154     710

  Fragments arranged by size: 

                 710     154      69
 
ApoI r'AATT_y

Cuts at:      0      25     933 
   Size:         25     908
 
BanII G_rGCy'C

Cuts at:      0     604     761     933 
   Size:        604     157     172

  Fragments arranged by size: 

                 604     172     157
 
BlpI GC'TnA_GC

Cuts at:      0     323     442     933 
   Size:        323     119     491

  Fragments arranged by size: 

                 491     323     119
 
Bpu10I CC'TnA_GC

Cuts at:      0     598     933 
   Size:        598     335
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     933 
   Size:        275     658
 
BseYI C'CCAG_C

Cuts at:      0     722     933 
   Size:        722     211
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     933 
   Size:         55     878
 
BsiHKAI G_wGCw'C

Cuts at:      0     494     604     671     933 
   Size:        494     110      67     262

  Fragments arranged by size: 

                 494     262     110      67
 
Bsp1286I G_dGCh'C

Cuts at:      0     494     604     671     761     933 
   Size:        494     110      67      90     172

  Fragments arranged by size: 

                 494     172     110      90      67
 
BspHI T'CATG_A

Cuts at:      0     632     933 
   Size:        632     301
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      54     128     721     933 
   Size:         54      74     593     212

  Fragments arranged by size: 

                 593     212      74      54
 
BsrDI GCAATG_nn'

Cuts at:      0     128     933 
   Size:        128     805
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      69     933 
   Size:         69     864
 
BstYI r'GATC_y

Cuts at:      0     201     502     933 
   Size:        201     301     431

  Fragments arranged by size: 

                 431     301     201
 
Bsu36I CC'TnA_GG

Cuts at:      0     771     933 
   Size:        771     162
 
EcoICRI GAG'CTC

Cuts at:      0     602     933 
   Size:        602     331
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     665     933 
   Size:        202     463     268

  Fragments arranged by size: 

                 463     268     202
 
EcoRI G'AATT_C

Cuts at:      0      25     933 
   Size:         25     908
 
HaeII r_GCGC'y

Cuts at:      0     109     933 
   Size:        109     824
 
MfeI C'AATT_G

Cuts at:      0     779     933 
   Size:        779     154
 
MslI CAynn'nnrTG

Cuts at:      0      16     166     933 
   Size:         16     150     767

  Fragments arranged by size: 

                 767     150      16
 
NspI r_CATG'y

Cuts at:      0     483     933 
   Size:        483     450
 
PciI A'CATG_T

Cuts at:      0     479     933 
   Size:        479     454
 
PstI C_TGCA'G

Cuts at:      0      65     933 
   Size:         65     868
 
SacI G_AGCT'C

Cuts at:      0     604     933 
   Size:        604     329
 
SfcI C'TryA_G

Cuts at:      0      61     933 
   Size:         61     872
 
SmlI C'TyrA_G

Cuts at:      0     290     933 
   Size:        290     643
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     180     933 
   Size:        180     753
 
TatI w'GTAC_w

Cuts at:      0     170     933 
   Size:        170     763
 
Tth111I GACn'n_nGTC

Cuts at:      0     689     933 
   Size:        689     244
 
XmnI GAAnn'nnTTC

Cuts at:      0     678     933 
   Size:        678     255
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI    AlwNI     ApoI    BanII
     BlpI   Bpu10I    BpuEI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrDI   BstAPI    BstYI   Bsu36I  EcoICRI  Eco57MI    EcoRI
    HaeII     MfeI     MslI     NspI     PciI     PstI     SacI     SfcI
     SmlI    TaqII     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI     XbaI     XcmI     XhoI     XmaI     ZraI