(Linear) (Six-base) MAPSORT of: KIEE5927.seq  Check: 7549  from: 1  to: 933



KIEE5927.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     674     933 
   Size:        674     259
 
AleI CACnn'nnGTG

Cuts at:      0     506     933 
   Size:        506     427
 
Bme1580I G_kGCm'C

Cuts at:      0     636     933 
   Size:        636     297
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      14     448     933 
   Size:         14     434     485

  Fragments arranged by size: 

                 485     434      14
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     815     933 
   Size:        815     118
 
BsaBI GATnn'nnATC

Cuts at:      0     822     933 
   Size:        822     111
 
BseYI C'CCAG_C

Cuts at:      0      45     933 
   Size:         45     888
 
BsiHKAI G_wGCw'C

Cuts at:      0      83     933 
   Size:         83     850
 
BsmI GAATG_Cn'

Cuts at:      0     712     933 
   Size:        712     221
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     636     933 
   Size:         83     553     297

  Fragments arranged by size: 

                 553     297      83
 
BspHI T'CATG_A

Cuts at:      0     344     593     644     677     933 
   Size:        344     249      51      33     256

  Fragments arranged by size: 

                 344     256     249      51      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     692     933 
   Size:        692     241
 
BsrGI T'GTAC_A

Cuts at:      0     585     933 
   Size:        585     348
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     508     933 
   Size:        508     425
 
BtgI C'CryG_G

Cuts at:      0     353     933 
   Size:        353     580
 
BtsI GCAGTG_nn'

Cuts at:      0     461     933 
   Size:        461     472
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     757     933 
   Size:         88     669     176

  Fragments arranged by size: 

                 669     176      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     674     815     933 
   Size:        674     141     118

  Fragments arranged by size: 

                 674     141     118
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     259     906     933 
   Size:        259     647      27

  Fragments arranged by size: 

                 647     259      27
 
EcoRV GAT'ATC

Cuts at:      0     650     933 
   Size:        650     283
 
HindIII A'AGCT_T

Cuts at:      0     807     933 
   Size:        807     126
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     933 
   Size:        275     658
 
MslI CAynn'nnrTG

Cuts at:      0      76     506     850     933 
   Size:         76     430     344      83

  Fragments arranged by size: 

                 430     344      83      76
 
MspA1I CmG'CkG

Cuts at:      0     742     933 
   Size:        742     191
 
NcoI C'CATG_G

Cuts at:      0     353     933 
   Size:        353     580
 
NspI r_CATG'y

Cuts at:      0     546     933 
   Size:        546     387
 
PvuII CAG'CTG

Cuts at:      0     742     933 
   Size:        742     191
 
SapI GCTCTTCn'nnn_

Cuts at:      0      88     933 
   Size:         88     845
 
ScaI AGT'ACT

Cuts at:      0     304     933 
   Size:        304     629
 
SspI AAT'ATT

Cuts at:      0      18     933 
   Size:         18     915
 
StyI C'CwwG_G

Cuts at:      0     353     707     933 
   Size:        353     354     226

  Fragments arranged by size: 

                 354     353     226
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     189     429     933 
   Size:        189     240     504

  Fragments arranged by size: 

                 504     240     189
 
TatI w'GTAC_w

Cuts at:      0     302     568     585     933 
   Size:        302     266      17     348

  Fragments arranged by size: 

                 348     302     266      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     615     647     933 
   Size:        615      32     286

  Fragments arranged by size: 

                 615     286      32
 

 Enzymes that do cut:

     AcuI     AleI Bme1580I     BmrI     BpmI    BsaBI    BseYI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrGI    BstXI     BtgI     BtsI
     EarI  Eco57MI    EcoNI    EcoRV  HindIII     MmeI     MslI   MspA1I
     NcoI     NspI    PvuII     SapI     ScaI     SspI     StyI    TaqII
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI