Restriction map for
pFN21AE5924
(Linear) (Six-base) MAPSORT of: KIEE5924.seq Check: 7034 from: 1 to: 933
KIEE5924.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 933
Size: 571 362
AlwNI CAG_nnn'CTG
Cuts at: 0 243 429 933
Size: 243 186 504
Fragments arranged by size:
504 243 186
ApaLI G'TGCA_C
Cuts at: 0 652 933
Size: 652 281
ApoI r'AATT_y
Cuts at: 0 811 933
Size: 811 122
AvaI C'yCGr_G
Cuts at: 0 602 933
Size: 602 331
BanII G_rGCy'C
Cuts at: 0 74 325 897 933
Size: 74 251 572 36
Fragments arranged by size:
572 251 74 36
BbvCI CC'TCA_GC
Cuts at: 0 673 933
Size: 673 260
BclI T'GATC_A
Cuts at: 0 245 933
Size: 245 688
BlpI GC'TnA_GC
Cuts at: 0 296 756 933
Size: 296 460 177
Fragments arranged by size:
460 296 177
Bme1580I G_kGCm'C
Cuts at: 0 236 656 933
Size: 236 420 277
Fragments arranged by size:
420 277 236
BmgBI CAC'GTC
Cuts at: 0 885 933
Size: 885 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 933
Size: 88 845
Bpu10I CC'TnA_GC
Cuts at: 0 673 933
Size: 673 260
BsaI GGTCTCn'nnnn_
Cuts at: 0 487 933
Size: 487 446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 275 305 933
Size: 275 30 628
Fragments arranged by size:
628 275 30
BseYI C'CCAG_C
Cuts at: 0 236 691 933
Size: 236 455 242
Fragments arranged by size:
455 242 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 933
Size: 148 785
BsiHKAI G_wGCw'C
Cuts at: 0 341 656 933
Size: 341 315 277
Fragments arranged by size:
341 315 277
Bsp1286I G_dGCh'C
Cuts at: 0 74 236 325 341 656 897 933
Size: 74 162 89 16 315 241 36
Fragments arranged by size:
315 241 162 89 74 36 16
BsrDI GCAATG_nn'
Cuts at: 0 494 656 933
Size: 494 162 277
Fragments arranged by size:
494 277 162
BtgI C'CryG_G
Cuts at: 0 279 933
Size: 279 654
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 933
Size: 120 813
BtsI GCAGTG_nn'
Cuts at: 0 674 933
Size: 674 259
DraIII CAC_nnn'GTG
Cuts at: 0 332 933
Size: 332 601
DrdI GACnn_nn'nnGTC
Cuts at: 0 720 933
Size: 720 213
EaeI y'GGCC_r
Cuts at: 0 58 398 491 507 933
Size: 58 340 93 16 426
Fragments arranged by size:
426 340 93 58 16
EarI CTCTTCn'nnn_
Cuts at: 0 307 933
Size: 307 626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 571 933
Size: 88 483 362
Fragments arranged by size:
483 362 88
EcoO109I rG'GnC_Cy
Cuts at: 0 722 933
Size: 722 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 596 628 933
Size: 596 32 305
Fragments arranged by size:
596 305 32
HincII GTy'rAC
Cuts at: 0 686 933
Size: 686 247
MscI TGG'CCA
Cuts at: 0 400 493 509 933
Size: 400 93 16 424
Fragments arranged by size:
424 400 93 16
MslI CAynn'nnrTG
Cuts at: 0 40 172 613 933
Size: 40 132 441 320
Fragments arranged by size:
441 320 132 40
PasI CC'CwG_GG
Cuts at: 0 318 933
Size: 318 615
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 429 933
Size: 396 33 504
Fragments arranged by size:
504 396 33
PfoI T'CCnGG_A
Cuts at: 0 203 933
Size: 203 730
PpuMI rG'GwC_Cy
Cuts at: 0 722 933
Size: 722 211
PstI C_TGCA'G
Cuts at: 0 630 933
Size: 630 303
SanDI GG'GwC_CC
Cuts at: 0 722 933
Size: 722 211
SapI GCTCTTCn'nnn_
Cuts at: 0 307 933
Size: 307 626
ScaI AGT'ACT
Cuts at: 0 908 933
Size: 908 25
SfcI C'TryA_G
Cuts at: 0 626 933
Size: 626 307
TatI w'GTAC_w
Cuts at: 0 176 906 933
Size: 176 730 27
Fragments arranged by size:
730 176 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 275 307 933
Size: 275 32 626
Fragments arranged by size:
626 275 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 594 933
Size: 594 339
XmnI GAAnn'nnTTC
Cuts at: 0 523 933
Size: 523 410
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI AvaI BanII BbvCI BclI
BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BsrDI BtgI BtgZI BtsI DraIII
DrdI EaeI EarI Eco57MI EcoO109I Hin4I HincII MscI
MslI PasI PflMI PfoI PpuMI PstI SanDI SapI
ScaI SfcI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BfrBI BglI BglII BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
ClaI CspCI DraI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI