Restriction map for
pFN21AE5923
(Linear) (Six-base) MAPSORT of: KIEE5923.seq Check: 5695 from: 1 to: 933
KIEE5923.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 933
Size: 721 212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 744 815 891 933
Size: 568 176 71 76 42
Fragments arranged by size:
568 176 76 71 42
AflIII A'CryG_T
Cuts at: 0 470 933
Size: 470 463
AleI CACnn'nnGTG
Cuts at: 0 832 933
Size: 832 101
AlwNI CAG_nnn'CTG
Cuts at: 0 817 933
Size: 817 116
ApaI G_GGCC'C
Cuts at: 0 894 933
Size: 894 39
ApoI r'AATT_y
Cuts at: 0 25 80 933
Size: 25 55 853
Fragments arranged by size:
853 55 25
BanI G'GyrC_C
Cuts at: 0 604 933
Size: 604 329
BanII G_rGCy'C
Cuts at: 0 894 933
Size: 894 39
BfrBI ATG'CAT
Cuts at: 0 632 933
Size: 632 301
Bme1580I G_kGCm'C
Cuts at: 0 420 894 933
Size: 420 474 39
Fragments arranged by size:
474 420 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 122 515 933
Size: 122 393 418
Fragments arranged by size:
418 393 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 933
Size: 38 895
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 593 623 933
Size: 253 30 310 30 310
Fragments arranged by size:
310 310 253 30 30
BseYI C'CCAG_C
Cuts at: 0 437 933
Size: 437 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 933
Size: 136 797
Bsp1286I G_dGCh'C
Cuts at: 0 420 894 933
Size: 420 474 39
Fragments arranged by size:
474 420 39
BspHI T'CATG_A
Cuts at: 0 398 933
Size: 398 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 158 721 933
Size: 128 30 563 212
Fragments arranged by size:
563 212 128 30
BsrDI GCAATG_nn'
Cuts at: 0 785 806 827 933
Size: 785 21 21 106
Fragments arranged by size:
785 106 21 21
BsrGI T'GTAC_A
Cuts at: 0 824 933
Size: 824 109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 817 933
Size: 817 116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 933
Size: 237 696
Bsu36I CC'TnA_GG
Cuts at: 0 670 868 933
Size: 670 198 65
Fragments arranged by size:
670 198 65
BtgI C'CryG_G
Cuts at: 0 344 684 933
Size: 344 340 249
Fragments arranged by size:
344 340 249
DraIII CAC_nnn'GTG
Cuts at: 0 657 933
Size: 657 276
EaeI y'GGCC_r
Cuts at: 0 134 182 365 933
Size: 134 48 183 568
Fragments arranged by size:
568 183 134 48
EarI CTCTTCn'nnn_
Cuts at: 0 79 763 791 910 933
Size: 79 684 28 119 23
Fragments arranged by size:
684 119 79 28 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 568 744 815 891 933
Size: 38 530 176 71 76 42
Fragments arranged by size:
530 176 76 71 42 38
EcoO109I rG'GnC_Cy
Cuts at: 0 404 441 752 890 891 933
Size: 404 37 311 138 1 42
Fragments arranged by size:
404 311 138 42 37 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 921 933
Size: 921 12
HaeII r_GCGC'y
Cuts at: 0 52 933
Size: 52 881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 933
Size: 243 32 658
Fragments arranged by size:
658 243 32
HincII GTy'rAC
Cuts at: 0 201 933
Size: 201 732
MscI TGG'CCA
Cuts at: 0 136 184 367 933
Size: 136 48 183 566
Fragments arranged by size:
566 183 136 48
MslI CAynn'nnrTG
Cuts at: 0 166 292 397 832 933
Size: 166 126 105 435 101
Fragments arranged by size:
435 166 126 105 101
MspA1I CmG'CkG
Cuts at: 0 817 933
Size: 817 116
NcoI C'CATG_G
Cuts at: 0 344 933
Size: 344 589
NsiI A_TGCA'T
Cuts at: 0 634 933
Size: 634 299
PpuMI rG'GwC_Cy
Cuts at: 0 441 752 933
Size: 441 311 181
Fragments arranged by size:
441 311 181
PspOMI G'GGCC_C
Cuts at: 0 890 933
Size: 890 43
PstI C_TGCA'G
Cuts at: 0 718 933
Size: 718 215
PvuII CAG'CTG
Cuts at: 0 817 933
Size: 817 116
SanDI GG'GwC_CC
Cuts at: 0 752 933
Size: 752 181
SapI GCTCTTCn'nnn_
Cuts at: 0 910 933
Size: 910 23
SfcI C'TryA_G
Cuts at: 0 714 861 933
Size: 714 147 72
Fragments arranged by size:
714 147 72
StuI AGG'CCT
Cuts at: 0 703 933
Size: 703 230
StyI C'CwwG_G
Cuts at: 0 344 933
Size: 344 589
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 583 933
Size: 583 350
TatI w'GTAC_w
Cuts at: 0 170 824 933
Size: 170 654 109
Fragments arranged by size:
654 170 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 253 285 933
Size: 253 32 648
Fragments arranged by size:
648 253 32
XbaI T'CTAG_A
Cuts at: 0 321 933
Size: 321 612
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApoI BanI
BanII BfrBI Bme1580I BmrI BpmI BsaXI BseYI BsgI
Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstXI Bsu36I
BtgI DraIII EaeI EarI Eco57MI EcoO109I FalI HaeII
Hin4I HincII MscI MslI MspA1I NcoI NsiI PpuMI
PspOMI PstI PvuII SanDI SapI SfcI StuI StyI
TaqII TatI TstI XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XcmI XhoI XmaI XmnI ZraI