(Linear) (Six-base) MAPSORT of: KIEE5922.seq  Check: 1376  from: 1  to: 933



KIEE5922.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:14  ..

AccI GT'mk_AC

Cuts at:      0     248     933 
   Size:        248     685
 
ApoI r'AATT_y

Cuts at:      0     805     933 
   Size:        805     128
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     795     933 
   Size:        795     138
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     564     933 
   Size:        564     369
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672     933 
   Size:        672     261
 
BsaWI w'CCGG_w

Cuts at:      0     410     933 
   Size:        410     523
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     405     435     933 
   Size:        405      30     498

  Fragments arranged by size: 

                 498     405      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     933 
   Size:        373     560
 
BsiHKAI G_wGCw'C

Cuts at:      0     266     933 
   Size:        266     667
 
Bsp1286I G_dGCh'C

Cuts at:      0     266     933 
   Size:        266     667
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     503     671     933 
   Size:        503     168     262

  Fragments arranged by size: 

                 503     262     168
 
BsrDI GCAATG_nn'

Cuts at:      0     129     933 
   Size:        129     804
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     519     933 
   Size:        519     414
 
ClaI AT'CG_AT

Cuts at:      0      19     933 
   Size:         19     914
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672     933 
   Size:        672     261
 
EcoO109I rG'GnC_Cy

Cuts at:      0     683     933 
   Size:        683     250
 
EcoRV GAT'ATC

Cuts at:      0     210     617     933 
   Size:        210     407     316

  Fragments arranged by size: 

                 407     316     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     894     926     933 
   Size:        894      32       7

  Fragments arranged by size: 

                 894      32       7
 
MslI CAynn'nnrTG

Cuts at:      0      11     733     933 
   Size:         11     722     200

  Fragments arranged by size: 

                 722     200      11
 
NdeI CA'TA_TG

Cuts at:      0     357     376     576     933 
   Size:        357      19     200     357

  Fragments arranged by size: 

                 357     357     200      19
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     505     933 
   Size:        505     428
 
PfoI T'CCnGG_A

Cuts at:      0     922     933 
   Size:        922      11
 
PpuMI rG'GwC_Cy

Cuts at:      0     683     933 
   Size:        683     250
 
SfcI C'TryA_G

Cuts at:      0      95     393     933 
   Size:         95     298     540

  Fragments arranged by size: 

                 540     298      95
 
StyI C'CwwG_G

Cuts at:      0     545     933 
   Size:        545     388
 
TatI w'GTAC_w

Cuts at:      0      36     176     397     832     933 
   Size:         36     140     221     435     101

  Fragments arranged by size: 

                 435     221     140     101      36
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     405     437     933 
   Size:        405      32     496

  Fragments arranged by size: 

                 496     405      32
 

 Enzymes that do cut:

     AccI     ApoI     BbsI    BciVI     BpmI    BsaWI    BsaXI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrDI    BstXI     ClaI  Eco57MI EcoO109I
    EcoRV    Hin4I     MslI     NdeI    PflMI     PfoI    PpuMI     SfcI
     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI