Restriction map for
pFN21AE5921
(Linear) (Six-base) MAPSORT of: KIEE5921.seq Check: 1693 from: 1 to: 933
KIEE5921.orf
With 182 enzymes: SgfI *
December 5, 2014 13:14 ..
AleI CACnn'nnGTG
Cuts at: 0 118 742 841 933
Size: 118 624 99 92
Fragments arranged by size:
624 118 99 92
ApoI r'AATT_y
Cuts at: 0 252 923 933
Size: 252 671 10
Fragments arranged by size:
671 252 10
BglI GCCn_nnn'nGGC
Cuts at: 0 355 933
Size: 355 578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 933
Size: 567 366
Bpu10I CC'TnA_GC
Cuts at: 0 65 612 933
Size: 65 547 321
Fragments arranged by size:
547 321 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 601 933
Size: 601 332
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 595 933
Size: 565 30 338
Fragments arranged by size:
565 338 30
BseYI C'CCAG_C
Cuts at: 0 545 933
Size: 545 388
BsiEI CG_ry'CG
Cuts at: 0 366 933
Size: 366 567
BsiHKAI G_wGCw'C
Cuts at: 0 134 680 933
Size: 134 546 253
Fragments arranged by size:
546 253 134
Bsp1286I G_dGCh'C
Cuts at: 0 134 680 933
Size: 134 546 253
Fragments arranged by size:
546 253 134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 665 933
Size: 350 315 268
Fragments arranged by size:
350 315 268
BsrDI GCAATG_nn'
Cuts at: 0 420 933
Size: 420 513
BsrFI r'CCGG_y
Cuts at: 0 365 933
Size: 365 568
BsrGI T'GTAC_A
Cuts at: 0 833 933
Size: 833 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 933
Size: 591 342
Bsu36I CC'TnA_GG
Cuts at: 0 30 877 933
Size: 30 847 56
Fragments arranged by size:
847 56 30
BtgI C'CryG_G
Cuts at: 0 415 933
Size: 415 518
BtsI GCAGTG_nn'
Cuts at: 0 113 933
Size: 113 820
EaeI y'GGCC_r
Cuts at: 0 143 356 374 933
Size: 143 213 18 559
Fragments arranged by size:
559 213 143 18
EarI CTCTTCn'nnn_
Cuts at: 0 60 933
Size: 60 873
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 933
Size: 567 366
EcoRV GAT'ATC
Cuts at: 0 213 933
Size: 213 720
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 933
Size: 565 32 336
Fragments arranged by size:
565 336 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 933
Size: 83 850
MscI TGG'CCA
Cuts at: 0 145 358 376 933
Size: 145 213 18 557
Fragments arranged by size:
557 213 145 18
MslI CAynn'nnrTG
Cuts at: 0 118 175 398 742 829 841 933
Size: 118 57 223 344 87 12 92
Fragments arranged by size:
344 223 118 92 87 57 12
NcoI C'CATG_G
Cuts at: 0 415 933
Size: 415 518
PflMI CCAn_nnn'nTGG
Cuts at: 0 594 704 933
Size: 594 110 229
Fragments arranged by size:
594 229 110
PstI C_TGCA'G
Cuts at: 0 74 696 933
Size: 74 622 237
Fragments arranged by size:
622 237 74
SfcI C'TryA_G
Cuts at: 0 70 692 740 870 933
Size: 70 622 48 130 63
Fragments arranged by size:
622 130 70 63 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 355 933
Size: 355 578
StuI AGG'CCT
Cuts at: 0 712 786 933
Size: 712 74 147
Fragments arranged by size:
712 147 74
StyI C'CwwG_G
Cuts at: 0 415 933
Size: 415 518
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 559 864 933
Size: 559 305 69
Fragments arranged by size:
559 305 69
TatI w'GTAC_w
Cuts at: 0 179 305 797 833 933
Size: 179 126 492 36 100
Fragments arranged by size:
492 179 126 100 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 451 483 933
Size: 451 32 450
Fragments arranged by size:
451 450 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 591 933
Size: 366 225 342
Fragments arranged by size:
366 342 225
XmnI GAAnn'nnTTC
Cuts at: 0 712 862 933
Size: 712 150 71
Fragments arranged by size:
712 150 71
Enzymes that do cut:
AleI ApoI BglI BpmI Bpu10I BsaI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstXI
Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoRV Hin4I
MmeI MscI MslI NcoI PflMI PstI SfcI SfiI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI ZraI