(Linear) (Six-base) MAPSORT of: KIEE5914.seq  Check: 2241  from: 1  to: 930



KIEE5914.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:13  ..

AccI GT'mk_AC

Cuts at:      0     203     930 
   Size:        203     727
 
AleI CACnn'nnGTG

Cuts at:      0     731     930 
   Size:        731     199
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     244     930 
   Size:        244     686
 
AlwNI CAG_nnn'CTG

Cuts at:      0     240     610     930 
   Size:        240     370     320

  Fragments arranged by size: 

                 370     320     240
 
AseI AT'TA_AT

Cuts at:      0     578     930 
   Size:        578     352
 
BanI G'GyrC_C

Cuts at:      0     228     695     930 
   Size:        228     467     235

  Fragments arranged by size: 

                 467     235     228
 
BanII G_rGCy'C

Cuts at:      0      86     140     782     796     930 
   Size:         86      54     642      14     134

  Fragments arranged by size: 

                 642     134      86      54      14
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     561     930 
   Size:        561     369
 
Bme1580I G_kGCm'C

Cuts at:      0     233     930 
   Size:        233     697
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      49     777     930 
   Size:         49     728     153

  Fragments arranged by size: 

                 728     153      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     930 
   Size:        138     792
 
BsaAI yAC'GTr

Cuts at:      0     366     930 
   Size:        366     564
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64     325     711     930 
   Size:         64     261     386     219

  Fragments arranged by size: 

                 386     261     219      64
 
BsiEI CG_ry'CG

Cuts at:      0     200     930 
   Size:        200     730
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     140     233     782     796     930 
   Size:         86      54      93     549      14     134

  Fragments arranged by size: 

                 549     134      93      86      54      14
 
BspHI T'CATG_A

Cuts at:      0     401     930 
   Size:        401     529
 
BstYI r'GATC_y

Cuts at:      0     677     930 
   Size:        677     253
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     192     930 
   Size:        192     738
 
BtsI GCAGTG_nn'

Cuts at:      0     611     930 
   Size:        611     319
 
EaeI y'GGCC_r

Cuts at:      0     368     930 
   Size:        368     562
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     841     913     930 
   Size:         91     750      72      17

  Fragments arranged by size: 

                 750      91      72      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     930 
   Size:        138     792
 
HincII GTy'rAC

Cuts at:      0     204     930 
   Size:        204     726
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     720     930 
   Size:        720     210
 
MscI TGG'CCA

Cuts at:      0     370     930 
   Size:        370     560
 
MslI CAynn'nnrTG

Cuts at:      0     169     578     731     766     930 
   Size:        169     409     153      35     164

  Fragments arranged by size: 

                 409     169     164     153      35
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     240     930 
   Size:        240     690
 
PfoI T'CCnGG_A

Cuts at:      0     439     930 
   Size:        439     491
 
SalI G'TCGA_C

Cuts at:      0     202     930 
   Size:        202     728
 
SapI GCTCTTCn'nnn_

Cuts at:      0      91     930 
   Size:         91     839
 
StyI C'CwwG_G

Cuts at:      0      58     797     888     930 
   Size:         58     739      91      42

  Fragments arranged by size: 

                 739      91      58      42
 
TatI w'GTAC_w

Cuts at:      0     173     930 
   Size:        173     757
 
XmnI GAAnn'nnTTC

Cuts at:      0     706     930 
   Size:        706     224
 

 Enzymes that do cut:

     AccI     AleI     AlfI    AlwNI     AseI     BanI    BanII    BciVI
 Bme1580I     BmrI     BpmI    BsaAI    BseRI    BsiEI Bsp1286I    BspHI
    BstYI    BtgZI     BtsI     EaeI     EarI  Eco57MI   HincII     MmeI
     MscI     MslI    PflMI     PfoI     SalI     SapI     StyI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI