(Linear) (Six-base) MAPSORT of: KIEE5912.seq  Check: 4581  from: 1  to: 930



KIEE5912.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:13  ..

AvaI C'yCGr_G

Cuts at:      0     407     930 
   Size:        407     523
 
BanII G_rGCy'C

Cuts at:      0      52     167     930 
   Size:         52     115     763

  Fragments arranged by size: 

                 763     115      52
 
BclI T'GATC_A

Cuts at:      0     147     930 
   Size:        147     783
 
Bme1580I G_kGCm'C

Cuts at:      0     510     930 
   Size:        510     420
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     436     930 
   Size:        436     494
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     930 
   Size:        640     290
 
BsaWI w'CCGG_w

Cuts at:      0      68     930 
   Size:         68     862
 
BsmI GAATG_Cn'

Cuts at:      0     392     930 
   Size:        392     538
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     167     510     930 
   Size:         52     115     343     420

  Fragments arranged by size: 

                 420     343     115      52
 
BsrDI GCAATG_nn'

Cuts at:      0     359     671     930 
   Size:        359     312     259

  Fragments arranged by size: 

                 359     312     259
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     237     930 
   Size:        237     693
 
BstYI r'GATC_y

Cuts at:      0      71     930 
   Size:         71     859
 
EaeI y'GGCC_r

Cuts at:      0     311     930 
   Size:        311     619
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     930 
   Size:        131     799
 
EcoRV GAT'ATC

Cuts at:      0     564     930 
   Size:        564     366
 
MscI TGG'CCA

Cuts at:      0     313     930 
   Size:        313     617
 
MslI CAynn'nnrTG

Cuts at:      0     223     536     751     754     814     930 
   Size:        223     313     215       3      60     116

  Fragments arranged by size: 

                 313     223     215     116      60       3
 
NspI r_CATG'y

Cuts at:      0     793     930 
   Size:        793     137
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     496     930 
   Size:        496     434
 
SmlI C'TyrA_G

Cuts at:      0     655     930 
   Size:        655     275
 
StyI C'CwwG_G

Cuts at:      0     204     930 
   Size:        204     726
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     229     304     930 
   Size:        229      75     626

  Fragments arranged by size: 

                 626     229      75
 
TatI w'GTAC_w

Cuts at:      0     170     930 
   Size:        170     760
 

 Enzymes that do cut:

     AvaI    BanII     BclI Bme1580I     BmrI    BpuEI    BsaWI     BsmI
 Bsp1286I    BsrDI    BstXI    BstYI     EaeI     EarI    EcoRV     MscI
     MslI     NspI    PflMI     SmlI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI