(Linear) (Six-base) MAPSORT of: KIEE5910.seq  Check: 6735  from: 1  to: 930



KIEE5910.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:13  ..

AccI GT'mk_AC

Cuts at:      0     336     930 
   Size:        336     594
 
AflIII A'CryG_T

Cuts at:      0     404     930 
   Size:        404     526
 
AleI CACnn'nnGTG

Cuts at:      0     838     930 
   Size:        838      92
 
AlwNI CAG_nnn'CTG

Cuts at:      0     328     930 
   Size:        328     602
 
ApaI G_GGCC'C

Cuts at:      0     236     930 
   Size:        236     694
 
ApoI r'AATT_y

Cuts at:      0     920     930 
   Size:        920      10
 
AseI AT'TA_AT

Cuts at:      0     110     521     930 
   Size:        110     411     409

  Fragments arranged by size: 

                 411     409     110
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     563     596     930 
   Size:        563      33     334

  Fragments arranged by size: 

                 563     334      33
 
BanII G_rGCy'C

Cuts at:      0     236     930 
   Size:        236     694
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     300     930 
   Size:        300     630
 
BbvCI CC'TCA_GC

Cuts at:      0     692     930 
   Size:        692     238
 
BglII A'GATC_T

Cuts at:      0     249     930 
   Size:        249     681
 
Bme1580I G_kGCm'C

Cuts at:      0     236     930 
   Size:        236     694
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      15     239     446     930 
   Size:         15     224     207     484

  Fragments arranged by size: 

                 484     224     207      15
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     702     930 
   Size:        702     228
 
Bpu10I CC'TnA_GC

Cuts at:      0     692     930 
   Size:        692     238
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       9     536     930 
   Size:          9     527     394

  Fragments arranged by size: 

                 527     394       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     294     930 
   Size:        294     636
 
Bsp1286I G_dGCh'C

Cuts at:      0     236     294     930 
   Size:        236      58     636

  Fragments arranged by size: 

                 636     236      58
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     419     930 
   Size:        419     511
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     243     930 
   Size:        243     687
 
BstYI r'GATC_y

Cuts at:      0     249     930 
   Size:        249     681
 
Bsu36I CC'TnA_GG

Cuts at:      0     874     930 
   Size:        874      56
 
EaeI y'GGCC_r

Cuts at:      0     781     930 
   Size:        781     149
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     481     930 
   Size:        481     449
 
EcoO109I rG'GnC_Cy

Cuts at:      0     897     930 
   Size:        897      33
 
EcoRV GAT'ATC

Cuts at:      0     540     930 
   Size:        540     390
 
HincII GTy'rAC

Cuts at:      0     402     930 
   Size:        402     528
 
MslI CAynn'nnrTG

Cuts at:      0     172     838     930 
   Size:        172     666      92

  Fragments arranged by size: 

                 666     172      92
 
NdeI CA'TA_TG

Cuts at:      0     476     930 
   Size:        476     454
 
NspI r_CATG'y

Cuts at:      0     408     722     930 
   Size:        408     314     208

  Fragments arranged by size: 

                 408     314     208
 
PciI A'CATG_T

Cuts at:      0     404     930 
   Size:        404     526
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     685     717     930 
   Size:        685      32     213

  Fragments arranged by size: 

                 685     213      32
 
PspOMI G'GGCC_C

Cuts at:      0     232     930 
   Size:        232     698
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     473     505     930 
   Size:        473      32     425

  Fragments arranged by size: 

                 473     425      32
 
XbaI T'CTAG_A

Cuts at:      0     801     930 
   Size:        801     129
 

 Enzymes that do cut:

     AccI   AflIII     AleI    AlwNI     ApaI     ApoI     AseI     BaeI
    BanII     BbsI    BbvCI    BglII Bme1580I     BmrI     BplI   Bpu10I
    BseRI  BsiHKAI Bsp1286I   BstAPI    BstXI    BstYI   Bsu36I     EaeI
     EciI EcoO109I    EcoRV   HincII     MslI     NdeI     NspI     PciI
     PpiI   PspOMI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    ApaLI     AscI    AsiSI     AvaI    AvrII
    BamHI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI
  Eco57MI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI