Restriction map for
pFN21AE5909
(Linear) (Six-base) MAPSORT of: KIEE5909.seq Check: 2560 from: 1 to: 930
KIEE5909.orf
With 182 enzymes: SgfI *
December 5, 2014 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 930
Size: 724 206
AatII G_ACGT'C
Cuts at: 0 212 930
Size: 212 718
Acc65I G'GTAC_C
Cuts at: 0 754 930
Size: 754 176
AleI CACnn'nnGTG
Cuts at: 0 22 736 930
Size: 22 714 194
Fragments arranged by size:
714 194 22
AlwNI CAG_nnn'CTG
Cuts at: 0 243 387 930
Size: 243 144 543
Fragments arranged by size:
543 243 144
ApoI r'AATT_y
Cuts at: 0 905 930
Size: 905 25
BamHI G'GATC_C
Cuts at: 0 680 930
Size: 680 250
BanI G'GyrC_C
Cuts at: 0 754 930
Size: 754 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 788 930
Size: 788 142
BglI GCCn_nnn'nGGC
Cuts at: 0 352 930
Size: 352 578
Bme1580I G_kGCm'C
Cuts at: 0 72 930
Size: 72 858
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 596 930
Size: 596 334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 930
Size: 277 653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 566 795 930
Size: 444 122 229 135
Fragments arranged by size:
444 229 135 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 460 930
Size: 460 470
BsaHI Gr'CG_yC
Cuts at: 0 209 930
Size: 209 721
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 536 930
Size: 536 394
BseYI C'CCAG_C
Cuts at: 0 436 930
Size: 436 494
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 930
Size: 702 228
BsmI GAATG_Cn'
Cuts at: 0 46 930
Size: 46 884
Bsp1286I G_dGCh'C
Cuts at: 0 72 930
Size: 72 858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 724 930
Size: 724 206
BstEII G'GTnAC_C
Cuts at: 0 24 930
Size: 24 906
BstYI r'GATC_y
Cuts at: 0 680 930
Size: 680 250
Bsu36I CC'TnA_GG
Cuts at: 0 871 930
Size: 871 59
BtgI C'CryG_G
Cuts at: 0 629 930
Size: 629 301
BtsI GCAGTG_nn'
Cuts at: 0 254 731 930
Size: 254 477 199
Fragments arranged by size:
477 254 199
DraIII CAC_nnn'GTG
Cuts at: 0 289 930
Size: 289 641
EaeI y'GGCC_r
Cuts at: 0 668 930
Size: 668 262
EarI CTCTTCn'nnn_
Cuts at: 0 85 751 930
Size: 85 666 179
Fragments arranged by size:
666 179 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 930
Size: 277 653
EcoO109I rG'GnC_Cy
Cuts at: 0 894 930
Size: 894 36
HincII GTy'rAC
Cuts at: 0 560 930
Size: 560 370
KpnI G_GTAC'C
Cuts at: 0 758 930
Size: 758 172
MscI TGG'CCA
Cuts at: 0 670 930
Size: 670 260
MslI CAynn'nnrTG
Cuts at: 0 22 634 736 766 844 930
Size: 22 612 102 30 78 86
Fragments arranged by size:
612 102 86 78 30 22
NcoI C'CATG_G
Cuts at: 0 629 930
Size: 629 301
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 930
Size: 243 687
SfcI C'TryA_G
Cuts at: 0 864 930
Size: 864 66
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 930
Size: 352 578
SmlI C'TyrA_G
Cuts at: 0 423 581 774 930
Size: 423 158 193 156
Fragments arranged by size:
423 193 158 156
StyI C'CwwG_G
Cuts at: 0 20 467 629 930
Size: 20 447 162 301
Fragments arranged by size:
447 301 162 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 43 930
Size: 43 887
TatI w'GTAC_w
Cuts at: 0 770 930
Size: 770 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 42 74 930
Size: 42 32 856
Fragments arranged by size:
856 42 32
ZraI GAC'GTC
Cuts at: 0 210 930
Size: 210 720
Enzymes that do cut:
AarI AatII Acc65I AleI AlwNI ApoI BamHI BanI
BbsI BglI Bme1580I BplI BpmI BpuEI BsaI BsaHI
BseRI BseYI BsgI BsmI Bsp1286I BspMI BstEII BstYI
Bsu36I BtgI BtsI DraIII EaeI EarI Eco57MI EcoO109I
HincII KpnI MscI MslI NcoI PflMI SfcI SfiI
SmlI StyI TaqII TatI TstI ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI Bpu10I BsaAI
BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI MfeI
MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI