(Linear) (Six-base) MAPSORT of: KIEE5908.seq  Check: 9116  from: 1  to: 930



KIEE5908.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     704     880     930 
   Size:        258     446     176      50

  Fragments arranged by size: 

                 446     258     176      50
 
AflII C'TTAA_G

Cuts at:      0     157     930 
   Size:        157     773
 
AflIII A'CryG_T

Cuts at:      0     709     930 
   Size:        709     221
 
BamHI G'GATC_C

Cuts at:      0      51     445     930 
   Size:         51     394     485

  Fragments arranged by size: 

                 485     394      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     265     930 
   Size:        265     665
 
BclI T'GATC_A

Cuts at:      0     512     911     930 
   Size:        512     399      19

  Fragments arranged by size: 

                 512     399      19
 
BglII A'GATC_T

Cuts at:      0     326     930 
   Size:        326     604
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     930 
   Size:        343     587
 
BsaBI GATnn'nnATC

Cuts at:      0     331     930 
   Size:        331     599
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     635     930 
   Size:        367     268     295

  Fragments arranged by size: 

                 367     295     268
 
BsmI GAATG_Cn'

Cuts at:      0     456     660     930 
   Size:        456     204     270

  Fragments arranged by size: 

                 456     270     204
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      97     930 
   Size:         97     833
 
BsrGI T'GTAC_A

Cuts at:      0     764     930 
   Size:        764     166
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     313     930 
   Size:        313     617
 
BstYI r'GATC_y

Cuts at:      0      51     326     445     930 
   Size:         51     275     119     485

  Fragments arranged by size: 

                 485     275     119      51
 
BtgI C'CryG_G

Cuts at:      0     791     930 
   Size:        791     139
 
BtsI GCAGTG_nn'

Cuts at:      0     623     930 
   Size:        623     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     343     704     880     930 
   Size:        258      85     361     176      50

  Fragments arranged by size: 

                 361     258     176      85      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      36      68     930 
   Size:         36      32     862

  Fragments arranged by size: 

                 862      36      32
 
HincII GTy'rAC

Cuts at:      0     354     579     930 
   Size:        354     225     351

  Fragments arranged by size: 

                 354     351     225
 
HindIII A'AGCT_T

Cuts at:      0     892     930 
   Size:        892      38
 
HpaI GTT'AAC

Cuts at:      0     579     930 
   Size:        579     351
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     804     930 
   Size:        804     126
 
MslI CAynn'nnrTG

Cuts at:      0     727     760     930 
   Size:        727      33     170

  Fragments arranged by size: 

                 727     170      33
 
NcoI C'CATG_G

Cuts at:      0     791     930 
   Size:        791     139
 
NspI r_CATG'y

Cuts at:      0     713     773     930 
   Size:        713      60     157

  Fragments arranged by size: 

                 713     157      60
 
PciI A'CATG_T

Cuts at:      0     709     930 
   Size:        709     221
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     499     930 
   Size:        499     431
 
SmlI C'TyrA_G

Cuts at:      0     157     930 
   Size:        157     773
 
SspI AAT'ATT

Cuts at:      0      71     638     930 
   Size:         71     567     292

  Fragments arranged by size: 

                 567     292      71
 
StyI C'CwwG_G

Cuts at:      0     791     930 
   Size:        791     139
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     429     930 
   Size:        429     501
 
TatI w'GTAC_w

Cuts at:      0     764     930 
   Size:        764     166
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     437     469     930 
   Size:        437      32     461

  Fragments arranged by size: 

                 461     437      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     412     930 
   Size:        412     518
 

 Enzymes that do cut:

     AcuI    AflII   AflIII    BamHI     BbsI     BclI    BglII     BpmI
    BsaBI     BsgI     BsmI    BsmBI    BsrGI    BstXI    BstYI     BtgI
     BtsI  Eco57MI    Hin4I   HincII  HindIII     HpaI     MmeI     MslI
     NcoI     NspI     PciI    PflMI     SmlI     SspI     StyI    TaqII
     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI