(Linear) (Six-base) MAPSORT of: KIEE5904.seq  Check: 6923  from: 1  to: 927



KIEE5904.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:13  ..

AccI GT'mk_AC

Cuts at:      0     851     927 
   Size:        851      76
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     899     927 
   Size:        206     693      28

  Fragments arranged by size: 

                 693     206      28
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156     927 
   Size:        156     771
 
ApoI r'AATT_y

Cuts at:      0      25     927 
   Size:         25     902
 
BanII G_rGCy'C

Cuts at:      0     163     927 
   Size:        163     764
 
BbvCI CC'TCA_GC

Cuts at:      0     869     927 
   Size:        869      58
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     651     927 
   Size:        651     276
 
BclI T'GATC_A

Cuts at:      0     650     927 
   Size:        650     277
 
BglII A'GATC_T

Cuts at:      0     661     927 
   Size:        661     266
 
Bme1580I G_kGCm'C

Cuts at:      0     752     927 
   Size:        752     175
 
Bpu10I CC'TnA_GC

Cuts at:      0     869     927 
   Size:        869      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     678     927 
   Size:        275     403     249

  Fragments arranged by size: 

                 403     275     249
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     586     927 
   Size:        586     341
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     676     706     927 
   Size:        676      30     221

  Fragments arranged by size: 

                 676     221      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     163     927 
   Size:        163     764
 
BsmI GAATG_Cn'

Cuts at:      0     416     927 
   Size:        416     511
 
Bsp1286I G_dGCh'C

Cuts at:      0     163     752     927 
   Size:        163     589     175

  Fragments arranged by size: 

                 589     175     163
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     128     927 
   Size:        128     799
 
BstEII G'GTnAC_C

Cuts at:      0     132     927 
   Size:        132     795
 
BstYI r'GATC_y

Cuts at:      0     661     927 
   Size:        661     266
 
BtgI C'CryG_G

Cuts at:      0     845     927 
   Size:        845      82
 
BtsI GCAGTG_nn'

Cuts at:      0     437     927 
   Size:        437     490
 
DraI TTT'AAA

Cuts at:      0     668     927 
   Size:        668     259
 
EaeI y'GGCC_r

Cuts at:      0     365     927 
   Size:        365     562
 
EcoICRI GAG'CTC

Cuts at:      0     161     927 
   Size:        161     766
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     899     927 
   Size:        206     693      28

  Fragments arranged by size: 

                 693     206      28
 
EcoRI G'AATT_C

Cuts at:      0      25     927 
   Size:         25     902
 
HindIII A'AGCT_T

Cuts at:      0     892     927 
   Size:        892      35
 
MscI TGG'CCA

Cuts at:      0     367     927 
   Size:        367     560
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      74     106     927 
   Size:         74      32     821

  Fragments arranged by size: 

                 821      74      32
 
SacI G_AGCT'C

Cuts at:      0     163     927 
   Size:        163     764
 
SmlI C'TyrA_G

Cuts at:      0     290     657     927 
   Size:        290     367     270

  Fragments arranged by size: 

                 367     290     270
 
StyI C'CwwG_G

Cuts at:      0     576     927 
   Size:        576     351
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     269     429     927 
   Size:        269     160     498

  Fragments arranged by size: 

                 498     269     160
 
TatI w'GTAC_w

Cuts at:      0     170     927 
   Size:        170     757
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     676     708     927 
   Size:        676      32     219

  Fragments arranged by size: 

                 676     219      32
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI    BanII    BbvCI    BciVI     BclI
    BglII Bme1580I   Bpu10I    BpuEI     BsaI    BsaXI  BsiHKAI     BsmI
 Bsp1286I    BspMI   BstEII    BstYI     BtgI     BtsI     DraI     EaeI
  EcoICRI  Eco57MI    EcoRI  HindIII     MscI     PsrI     SacI     SmlI
     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
     BcgI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI