Restriction map for
pFN21AE5901
(Linear) (Six-base) MAPSORT of: KIEE5901.seq Check: 2526 from: 1 to: 927
KIEE5901.orf
With 182 enzymes: SgfI *
December 5, 2014 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 927
Size: 727 200
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 927
Size: 696 231
AflIII A'CryG_T
Cuts at: 0 770 927
Size: 770 157
AleI CACnn'nnGTG
Cuts at: 0 838 927
Size: 838 89
AlwNI CAG_nnn'CTG
Cuts at: 0 719 927
Size: 719 208
AvaI C'yCGr_G
Cuts at: 0 915 927
Size: 915 12
BamHI G'GATC_C
Cuts at: 0 153 927
Size: 153 774
BanI G'GyrC_C
Cuts at: 0 757 927
Size: 757 170
BanII G_rGCy'C
Cuts at: 0 325 927
Size: 325 602
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 927
Size: 274 653
BbvCI CC'TCA_GC
Cuts at: 0 676 927
Size: 676 251
BciVI GTATCCnnnnn_n'
Cuts at: 0 346 927
Size: 346 581
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 328 927
Size: 166 162 599
Fragments arranged by size:
599 166 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 927
Size: 88 839
Bpu10I CC'TnA_GC
Cuts at: 0 676 927
Size: 676 251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 650 927
Size: 538 112 277
Fragments arranged by size:
538 277 112
BsaAI yAC'GTr
Cuts at: 0 771 927
Size: 771 156
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 224 714 927
Size: 224 490 213
Fragments arranged by size:
490 224 213
Bsp1286I G_dGCh'C
Cuts at: 0 325 927
Size: 325 602
BspHI T'CATG_A
Cuts at: 0 404 927
Size: 404 523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 785 927
Size: 727 58 142
Fragments arranged by size:
727 142 58
BsrDI GCAATG_nn'
Cuts at: 0 18 368 927
Size: 18 350 559
Fragments arranged by size:
559 350 18
BstEII G'GTnAC_C
Cuts at: 0 840 927
Size: 840 87
BstYI r'GATC_y
Cuts at: 0 153 927
Size: 153 774
BtgI C'CryG_G
Cuts at: 0 412 836 927
Size: 412 424 91
Fragments arranged by size:
424 412 91
DraI TTT'AAA
Cuts at: 0 854 927
Size: 854 73
DrdI GACnn_nn'nnGTC
Cuts at: 0 577 927
Size: 577 350
EarI CTCTTCn'nnn_
Cuts at: 0 167 554 832 927
Size: 167 387 278 95
Fragments arranged by size:
387 278 167 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 696 927
Size: 88 608 231
Fragments arranged by size:
608 231 88
HincII GTy'rAC
Cuts at: 0 249 927
Size: 249 678
HpaI GTT'AAC
Cuts at: 0 249 927
Size: 249 678
MfeI C'AATT_G
Cuts at: 0 493 927
Size: 493 434
MslI CAynn'nnrTG
Cuts at: 0 838 927
Size: 838 89
MspA1I CmG'CkG
Cuts at: 0 429 927
Size: 429 498
NcoI C'CATG_G
Cuts at: 0 836 927
Size: 836 91
PflMI CCAn_nnn'nTGG
Cuts at: 0 429 519 927
Size: 429 90 408
Fragments arranged by size:
429 408 90
PvuII CAG'CTG
Cuts at: 0 429 927
Size: 429 498
SapI GCTCTTCn'nnn_
Cuts at: 0 554 927
Size: 554 373
SmlI C'TyrA_G
Cuts at: 0 553 665 927
Size: 553 112 262
Fragments arranged by size:
553 262 112
StyI C'CwwG_G
Cuts at: 0 356 807 836 927
Size: 356 451 29 91
Fragments arranged by size:
451 356 91 29
TatI w'GTAC_w
Cuts at: 0 176 927
Size: 176 751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 840 872 927
Size: 840 32 55
Fragments arranged by size:
840 55 32
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI AvaI BamHI BanI
BanII BbsI BbvCI BciVI BmrI BpmI Bpu10I BpuEI
BsaAI BseRI Bsp1286I BspHI BspMI BsrDI BstEII BstYI
BtgI DraI DrdI EarI Eco57MI HincII HpaI MfeI
MslI MspA1I NcoI PflMI PvuII SapI SmlI StyI
TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI BsaI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII
KasI KpnI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI