Restriction map for
pFN21AE5847
(Linear) (Six-base) MAPSORT of: KIEE5847.seq Check: 9442 from: 1 to: 6759
KIEE5847.orf
With 182 enzymes: SgfI *
December 5, 2014 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 768 3467 6759
Size: 768 2699 3292
Fragments arranged by size:
3292 2699 768
AccI GT'mk_AC
Cuts at: 0 338 2083 5418 6305 6759
Size: 338 1745 3335 887 454
Fragments arranged by size:
3335 1745 887 454 338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1275 1478 1635 2607 4890 5867 6733 6759
Size: 1275 203 157 972 2283 977 866 26
Fragments arranged by size:
2283 1275 977 972 866 203 157 26
AfeI AGC'GCT
Cuts at: 0 3068 6759
Size: 3068 3691
AflII C'TTAA_G
Cuts at: 0 4690 6759
Size: 4690 2069
AflIII A'CryG_T
Cuts at: 0 1280 1381 2579 3317 4109 6759
Size: 1280 101 1198 738 792 2650
Fragments arranged by size:
2650 1280 1198 792 738 101
AleI CACnn'nnGTG
Cuts at: 0 2773 6759
Size: 2773 3986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3475 5860 6759
Size: 3475 2385 899
Fragments arranged by size:
3475 2385 899
AlwNI CAG_nnn'CTG
Cuts at: 0 230 6759
Size: 230 6529
ApaI G_GGCC'C
Cuts at: 0 14 1240 6759
Size: 14 1226 5519
Fragments arranged by size:
5519 1226 14
ApaLI G'TGCA_C
Cuts at: 0 3388 4474 6759
Size: 3388 1086 2285
Fragments arranged by size:
3388 2285 1086
ApoI r'AATT_y
Cuts at: 0 1735 2019 2302 3287 4614 5459 6418 6759
Size: 1735 284 283 985 1327 845 959 341
Fragments arranged by size:
1735 1327 985 959 845 341 284 283
AseI AT'TA_AT
Cuts at: 0 1703 4327 6759
Size: 1703 2624 2432
Fragments arranged by size:
2624 2432 1703
AvaI C'yCGr_G
Cuts at: 0 135 142 406 844 955 1201 1233 4600
Size: 135 7 264 438 111 246 32 3367
Cuts at: 4600 6759
Size: 2159
Fragments arranged by size:
3367 2159 438 264 246 135 111 32
7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2353 2386 6759
Size: 2353 33 4373
Fragments arranged by size:
4373 2353 33
BamHI G'GATC_C
Cuts at: 0 3326 6759
Size: 3326 3433
BanI G'GyrC_C
Cuts at: 0 108 130 410 451 682 807 3087 3246
Size: 108 22 280 41 231 125 2280 159
Cuts at: 3246 6759
Size: 3513
Fragments arranged by size:
3513 2280 280 231 159 125 108 41
22
BanII G_rGCy'C
Cuts at: 0 14 1240 6759
Size: 14 1226 5519
Fragments arranged by size:
5519 1226 14
BbeI G_GCGC'C
Cuts at: 0 112 134 414 455 686 811 6759
Size: 112 22 280 41 231 125 5948
Fragments arranged by size:
5948 280 231 125 112 41 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 1481 1641 2944 3376 5279 5501 5734 6110
Size: 1481 160 1303 432 1903 222 233 376
Cuts at: 6110 6196 6721 6759
Size: 86 525 38
Fragments arranged by size:
1903 1481 1303 525 432 376 233 222
160 86 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3232 3266 6759
Size: 3232 34 3493
Fragments arranged by size:
3493 3232 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3240 5009 6759
Size: 3240 1769 1750
Fragments arranged by size:
3240 1769 1750
BclI T'GATC_A
Cuts at: 0 2757 4638 6488 6759
Size: 2757 1881 1850 271
Fragments arranged by size:
2757 1881 1850 271
BfrBI ATG'CAT
Cuts at: 0 4995 6759
Size: 4995 1764
BglI GCCn_nnn'nGGC
Cuts at: 0 463 6247 6759
Size: 463 5784 512
Fragments arranged by size:
5784 512 463
BlpI GC'TnA_GC
Cuts at: 0 289 3200 4192 6759
Size: 289 2911 992 2567
Fragments arranged by size:
2911 2567 992 289
Bme1580I G_kGCm'C
Cuts at: 0 14 90 550 794 1240 3126 3392 3436
Size: 14 76 460 244 446 1886 266 44
Cuts at: 3436 4478 6759
Size: 1042 2281
Fragments arranged by size:
2281 1886 1042 460 446 266 244 76
44 14
BmgBI CAC'GTC
Cuts at: 0 380 1079 1773 3786 6759
Size: 380 699 694 2013 2973
Fragments arranged by size:
2973 2013 699 694 380
BmrI ACTGGGnnnn_n'
Cuts at: 0 762 4437 6759
Size: 762 3675 2322
Fragments arranged by size:
3675 2322 762
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2212 4379 6759
Size: 2212 2167 2380
Fragments arranged by size:
2380 2212 2167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 4144 4666 5654 6759
Size: 1102 3042 522 988 1105
Fragments arranged by size:
3042 1105 1102 988 522
Bpu10I CC'TnA_GC
Cuts at: 0 40 46 211 697 2980 4469 6759
Size: 40 6 165 486 2283 1489 2290
Fragments arranged by size:
2290 2283 1489 486 165 40 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4349 4461 5564 6759
Size: 4349 112 1103 1195
Fragments arranged by size:
4349 1195 1103 112
BsaI GGTCTCn'nnnn_
Cuts at: 0 4588 6759
Size: 4588 2171
BsaAI yAC'GTr
Cuts at: 0 1281 1347 3591 6759
Size: 1281 66 2244 3168
Fragments arranged by size:
3168 2244 1281 66
BsaBI GATnn'nnATC
Cuts at: 0 3493 3671 5179 5774 6759
Size: 3493 178 1508 595 985
Fragments arranged by size:
3493 1508 985 595 178
BsaHI Gr'CG_yC
Cuts at: 0 109 131 411 452 683 808 974 6759
Size: 109 22 280 41 231 125 166 5785
Fragments arranged by size:
5785 280 231 166 125 109 41 22
BsaWI w'CCGG_w
Cuts at: 0 4097 6759
Size: 4097 2662
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 893 923 1100 1130 3333 3363 6759
Size: 893 30 177 30 2203 30 3396
Fragments arranged by size:
3396 2203 893 177 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 112 157 2456 4379 6565 6759
Size: 10 102 45 2299 1923 2186 194
Fragments arranged by size:
2299 2186 1923 194 102 45 10
BseYI C'CCAG_C
Cuts at: 0 181 518 839 3135 3429 6759
Size: 181 337 321 2296 294 3330
Fragments arranged by size:
3330 2296 337 321 294 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 3126 4894 5830 6759
Size: 754 2372 1768 936 929
Fragments arranged by size:
2372 1768 936 929 754
BsiEI CG_ry'CG
Cuts at: 0 57 159 219 317 366 752 3916 6759
Size: 57 102 60 98 49 386 3164 2843
Fragments arranged by size:
3164 2843 386 102 98 60 57 49
BsiHKAI G_wGCw'C
Cuts at: 0 1352 3392 4427 4478 5121 6044 6759
Size: 1352 2040 1035 51 643 923 715
Fragments arranged by size:
2040 1352 1035 923 715 643 51
BsmI GAATG_Cn'
Cuts at: 0 1656 1699 3356 3452 4991 6759
Size: 1656 43 1657 96 1539 1768
Fragments arranged by size:
1768 1657 1656 1539 96 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 105 489 6759
Size: 105 384 6270
Fragments arranged by size:
6270 384 105
Bsp1286I G_dGCh'C
Cuts at: 0 14 90 550 794 1240 1352 3126 3392
Size: 14 76 460 244 446 112 1774 266
Cuts at: 3392 3436 4427 4478 5121 6044 6759
Size: 44 991 51 643 923 715
Fragments arranged by size:
1774 991 923 715 643 460 446 266
244 112 76 51 44 14
BspEI T'CCGG_A
Cuts at: 0 4097 6759
Size: 4097 2662
BspHI T'CATG_A
Cuts at: 0 2267 3093 3260 6759
Size: 2267 826 167 3499
Fragments arranged by size:
3499 2267 826 167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 768 2107 2121 3467 4506 5430 6759
Size: 350 418 1339 14 1346 1039 924 1329
Fragments arranged by size:
1346 1339 1329 1039 924 418 350 14
BsrBI CCG'CTC
Cuts at: 0 16 215 6759
Size: 16 199 6544
Fragments arranged by size:
6544 199 16
BsrDI GCAATG_nn'
Cuts at: 0 2074 4190 5596 6581 6759
Size: 2074 2116 1406 985 178
Fragments arranged by size:
2116 2074 1406 985 178
BsrFI r'CCGG_y
Cuts at: 0 72 312 6759
Size: 72 240 6447
Fragments arranged by size:
6447 240 72
BsrGI T'GTAC_A
Cuts at: 0 3038 6759
Size: 3038 3721
BssHII G'CGCG_C
Cuts at: 0 355 565 754 756 6759
Size: 355 210 189 2 6003
Fragments arranged by size:
6003 355 210 189 2
BssSI C'ACGA_G
Cuts at: 0 5046 6759
Size: 5046 1713
BstAPI GCAn_nnn'nTGC
Cuts at: 0 230 545 4498 6759
Size: 230 315 3953 2261
Fragments arranged by size:
3953 2261 315 230
BstEII G'GTnAC_C
Cuts at: 0 6319 6759
Size: 6319 440
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3069 3084 6759
Size: 3069 15 3675
Fragments arranged by size:
3675 3069 15
BstYI r'GATC_y
Cuts at: 0 831 1143 2484 3326 3510 4371 4677 5247
Size: 831 312 1341 842 184 861 306 570
Cuts at: 5247 5629 6759
Size: 382 1130
Fragments arranged by size:
1341 1130 861 842 831 570 382 312
306 184
BstZ17I GTA'TAC
Cuts at: 0 6306 6759
Size: 6306 453
Bsu36I CC'TnA_GG
Cuts at: 0 124 4538 6196 6759
Size: 124 4414 1658 563
Fragments arranged by size:
4414 1658 563 124
BtgI C'CryG_G
Cuts at: 0 219 240 473 511 797 1674 2060 6759
Size: 219 21 233 38 286 877 386 4699
Fragments arranged by size:
4699 877 386 286 233 219 38 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 747 1040 6759
Size: 747 293 5719
Fragments arranged by size:
5719 747 293
BtsI GCAGTG_nn'
Cuts at: 0 2637 2682 3390 6759
Size: 2637 45 708 3369
Fragments arranged by size:
3369 2637 708 45
ClaI AT'CG_AT
Cuts at: 0 2213 6759
Size: 2213 4546
DraI TTT'AAA
Cuts at: 0 6014 6759
Size: 6014 745
DraIII CAC_nnn'GTG
Cuts at: 0 545 3474 6759
Size: 545 2929 3285
Fragments arranged by size:
3285 2929 545
DrdI GACnn_nn'nnGTC
Cuts at: 0 280 5029 6759
Size: 280 4749 1730
Fragments arranged by size:
4749 1730 280
EaeI y'GGCC_r
Cuts at: 0 54 156 216 310 314 363 749 2256
Size: 54 102 60 94 4 49 386 1507
Cuts at: 2256 3479 5906 6065 6759
Size: 1223 2427 159 694
Fragments arranged by size:
2427 1507 1223 694 386 159 102 94
60 54 49 4
EagI C'GGCC_G
Cuts at: 0 54 156 216 314 363 749 6759
Size: 54 102 60 98 49 386 6010
Fragments arranged by size:
6010 386 102 98 60 54 49
EarI CTCTTCn'nnn_
Cuts at: 0 2626 3565 4205 5095 5109 5448 6759
Size: 2626 939 640 890 14 339 1311
Fragments arranged by size:
2626 1311 939 890 640 339 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 418 486 724 6759
Size: 418 68 238 6035
Fragments arranged by size:
6035 418 238 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 1275 1478 1635 2607 4144 4666 4890
Size: 1102 173 203 157 972 1537 522 224
Cuts at: 4890 5654 5867 6733 6759
Size: 764 213 866 26
Fragments arranged by size:
1537 1102 972 866 764 522 224 213
203 173 157 26
EcoO109I rG'GnC_Cy
Cuts at: 0 1237 1870 2905 2927 6444 6759
Size: 1237 633 1035 22 3517 315
Fragments arranged by size:
3517 1237 1035 633 315 22
EcoRI G'AATT_C
Cuts at: 0 2302 6759
Size: 2302 4457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2875 6759
Size: 2875 3884
FseI GG_CCGG'CC
Cuts at: 0 316 6759
Size: 316 6443
HaeII r_GCGC'y
Cuts at: 0 112 134 206 261 296 414 455 686
Size: 112 22 72 55 35 118 41 231
Cuts at: 686 811 3070 6759
Size: 125 2259 3689
Fragments arranged by size:
3689 2259 231 125 118 112 72 55
41 35 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2451 2483 3486 3518 3818 3850 3898 3930
Size: 2451 32 1003 32 300 32 48 32
Cuts at: 3930 4077 4109 5224 5256 5630 5662 6541 6573
Size: 147 32 1115 32 374 32 879 32
Cuts at: 6573 6759
Size: 186
Fragments arranged by size:
2451 1115 1003 879 374 300 186 147
48 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 339 588 4949 5829 6759
Size: 339 249 4361 880 930
Fragments arranged by size:
4361 930 880 339 249
HindIII A'AGCT_T
Cuts at: 0 1568 5879 6759
Size: 1568 4311 880
Fragments arranged by size:
4311 1568 880
KasI G'GCGC_C
Cuts at: 0 108 130 410 451 682 807 6759
Size: 108 22 280 41 231 125 5952
Fragments arranged by size:
5952 280 231 125 108 41 22
MfeI C'AATT_G
Cuts at: 0 5712 6759
Size: 5712 1047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 2683 4958 5356 6759
Size: 1609 1074 2275 398 1403
Fragments arranged by size:
2275 1609 1403 1074 398
MscI TGG'CCA
Cuts at: 0 2258 3481 5908 6067 6759
Size: 2258 1223 2427 159 692
Fragments arranged by size:
2427 2258 1223 692 159
MslI CAynn'nnrTG
Cuts at: 0 2557 2773 3889 4043 5050 5624 6469 6759
Size: 2557 216 1116 154 1007 574 845 290
Fragments arranged by size:
2557 1116 1007 845 574 290 216 154
MspA1I CmG'CkG
Cuts at: 0 66 221 475 513 738 1059 3377 3429
Size: 66 155 254 38 225 321 2318 52
Cuts at: 3429 5670 6759
Size: 2241 1089
Fragments arranged by size:
2318 2241 1089 321 254 225 155 66
52 38
NaeI GCC'GGC
Cuts at: 0 314 6759
Size: 314 6445
NarI GG'CG_CC
Cuts at: 0 109 131 411 452 683 808 6759
Size: 109 22 280 41 231 125 5951
Fragments arranged by size:
5951 280 231 125 109 41 22
NcoI C'CATG_G
Cuts at: 0 240 1674 6759
Size: 240 1434 5085
Fragments arranged by size:
5085 1434 240
NdeI CA'TA_TG
Cuts at: 0 3982 6522 6759
Size: 3982 2540 237
Fragments arranged by size:
3982 2540 237
NgoMIV G'CCGG_C
Cuts at: 0 312 6759
Size: 312 6447
NotI GC'GGCC_GC
Cuts at: 0 156 216 363 6759
Size: 156 60 147 6396
Fragments arranged by size:
6396 156 147 60
NsiI A_TGCA'T
Cuts at: 0 4997 6759
Size: 4997 1762
NspI r_CATG'y
Cuts at: 0 705 1385 1628 1993 2583 3321 3652 4113
Size: 705 680 243 365 590 738 331 461
Cuts at: 4113 4456 6669 6759
Size: 343 2213 90
Fragments arranged by size:
2213 738 705 680 590 461 365 343
331 243 90
PasI CC'CwG_GG
Cuts at: 0 1226 1848 4481 6759
Size: 1226 622 2633 2278
Fragments arranged by size:
2633 2278 1226 622
PciI A'CATG_T
Cuts at: 0 1381 2579 3317 4109 6759
Size: 1381 1198 738 792 2650
Fragments arranged by size:
2650 1381 1198 792 738
PflMI CCAn_nnn'nTGG
Cuts at: 0 3312 6759
Size: 3312 3447
PmlI CAC'GTG
Cuts at: 0 1281 1347 6759
Size: 1281 66 5412
Fragments arranged by size:
5412 1281 66
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6637 6669 6759
Size: 6637 32 90
Fragments arranged by size:
6637 90 32
PpuMI rG'GwC_Cy
Cuts at: 0 2905 2927 6444 6759
Size: 2905 22 3517 315
Fragments arranged by size:
3517 2905 315 22
PshAI GACnn'nnGTC
Cuts at: 0 1645 6759
Size: 1645 5114
PsiI TTA'TAA
Cuts at: 0 5573 6759
Size: 5573 1186
PspOMI G'GGCC_C
Cuts at: 0 10 1236 6759
Size: 10 1226 5523
Fragments arranged by size:
5523 1226 10
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3221 3253 3759 3791 6759
Size: 3221 32 506 32 2968
Fragments arranged by size:
3221 2968 506 32 32
PstI C_TGCA'G
Cuts at: 0 347 1010 2118 2644 3245 6759
Size: 347 663 1108 526 601 3514
Fragments arranged by size:
3514 1108 663 601 526 347
PvuI CG_AT'CG
Cuts at: 0 3916 6759
Size: 3916 2843
PvuII CAG'CTG
Cuts at: 0 738 1059 3377 3429 6759
Size: 738 321 2318 52 3330
Fragments arranged by size:
3330 2318 738 321 52
SacII CC_GC'GG
Cuts at: 0 222 476 514 6759
Size: 222 254 38 6245
Fragments arranged by size:
6245 254 222 38
SalI G'TCGA_C
Cuts at: 0 337 6759
Size: 337 6422
SanDI GG'GwC_CC
Cuts at: 0 2905 6759
Size: 2905 3854
SapI GCTCTTCn'nnn_
Cuts at: 0 2626 5109 6759
Size: 2626 2483 1650
Fragments arranged by size:
2626 2483 1650
SbfI CC_TGCA'GG
Cuts at: 0 1010 2118 6759
Size: 1010 1108 4641
Fragments arranged by size:
4641 1108 1010
ScaI AGT'ACT
Cuts at: 0 772 2421 6759
Size: 772 1649 4338
Fragments arranged by size:
4338 1649 772
SexAI A'CCwGG_T
Cuts at: 0 6323 6759
Size: 6323 436
SfcI C'TryA_G
Cuts at: 0 343 1006 2114 2640 3241 5280 5543 5784
Size: 343 663 1108 526 601 2039 263 241
Cuts at: 5784 6351 6759
Size: 567 408
Fragments arranged by size:
2039 1108 663 601 567 526 408 343
263 241
SfoI GGC'GCC
Cuts at: 0 110 132 412 453 684 809 6759
Size: 110 22 280 41 231 125 5950
Fragments arranged by size:
5950 280 231 125 110 41 22
SmaI CCC'GGG
Cuts at: 0 137 408 6759
Size: 137 271 6351
Fragments arranged by size:
6351 271 137
SmlI C'TyrA_G
Cuts at: 0 844 4364 4440 4690 5579 6759
Size: 844 3520 76 250 889 1180
Fragments arranged by size:
3520 1180 889 844 250 76
SphI G_CATG'C
Cuts at: 0 705 1993 4456 6759
Size: 705 1288 2463 2303
Fragments arranged by size:
2463 2303 1288 705
SrfI GCCC'GGGC
Cuts at: 0 408 6759
Size: 408 6351
SspI AAT'ATT
Cuts at: 0 2448 5087 6759
Size: 2448 2639 1672
Fragments arranged by size:
2639 2448 1672
StuI AGG'CCT
Cuts at: 0 6 6759
Size: 6 6753
StyI C'CwwG_G
Cuts at: 0 240 827 1353 1674 2746 3482 4717 5364
Size: 240 587 526 321 1072 736 1235 647
Cuts at: 5364 6759
Size: 1395
Fragments arranged by size:
1395 1235 1072 736 647 587 526 321
240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1635 5784 6759
Size: 1635 4149 975
Fragments arranged by size:
4149 1635 975
TatI w'GTAC_w
Cuts at: 0 770 1242 1862 2419 3038 3223 6759
Size: 770 472 620 557 619 185 3536
Fragments arranged by size:
3536 770 620 619 557 472 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 893 925 3318 3350 6759
Size: 893 32 2393 32 3409
Fragments arranged by size:
3409 2393 893 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 381 976 2188 6759
Size: 381 595 1212 4571
Fragments arranged by size:
4571 1212 595 381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1863 6759
Size: 1863 4896
XhoI C'TCGA_G
Cuts at: 0 844 6759
Size: 844 5915
XmaI C'CCGG_G
Cuts at: 0 135 406 6759
Size: 135 271 6353
Fragments arranged by size:
6353 271 135
XmnI GAAnn'nnTTC
Cuts at: 0 5880 6058 6759
Size: 5880 178 701
Fragments arranged by size:
5880 701 178
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FseI HaeII Hin4I
HincII HindIII KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NotI NsiI NspI
PasI PciI PflMI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SmaI SmlI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AloI AscI AsiSI
AvrII BbvCI BglII BmtI BsiWI BstBI CspCI EcoICRI
EcoNI EcoRV FspI FspAI HpaI KpnI MluI NheI
NruI PacI PfoI PmeI PspXI RsrII SacI SfiI
SgfI SgrAI SnaBI SpeI SwaI XbaI ZraI