Restriction map for
pFN21AE5840
(Linear) (Six-base) MAPSORT of: KIEE5840.seq Check: 1511 from: 1 to: 7851
KIEE5840.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3181 4030 4750 5352 7851
Size: 3181 849 720 602 2499
Fragments arranged by size:
3181 2499 849 720 602
AatII G_ACGT'C
Cuts at: 0 3402 7851
Size: 3402 4449
AccI GT'mk_AC
Cuts at: 0 3413 5736 7851
Size: 3413 2323 2115
Fragments arranged by size:
3413 2323 2115
Acc65I G'GTAC_C
Cuts at: 0 5389 6438 7851
Size: 5389 1049 1413
Fragments arranged by size:
5389 1413 1049
AclI AA'CG_TT
Cuts at: 0 5461 7045 7851
Size: 5461 1584 806
Fragments arranged by size:
5461 1584 806
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1574 1814 2366 2836 3113 6524 6621 6795
Size: 1574 240 552 470 277 3411 97 174
Cuts at: 6795 7568 7838 7851
Size: 773 270 13
Fragments arranged by size:
3411 1574 773 552 470 277 270 240
174 97 13
AflII C'TTAA_G
Cuts at: 0 2401 7851
Size: 2401 5450
AflIII A'CryG_T
Cuts at: 0 1248 1347 2148 3603 7851
Size: 1248 99 801 1455 4248
Fragments arranged by size:
4248 1455 1248 801 99
AhdI GACnn_n'nnGTC
Cuts at: 0 297 2598 7851
Size: 297 2301 5253
Fragments arranged by size:
5253 2301 297
AleI CACnn'nnGTG
Cuts at: 0 2101 4576 7851
Size: 2101 2475 3275
Fragments arranged by size:
3275 2475 2101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2183 4058 4361 5903 6165 7851
Size: 2183 1875 303 1542 262 1686
Fragments arranged by size:
2183 1875 1686 1542 303 262
AlwNI CAG_nnn'CTG
Cuts at: 0 505 1585 1774 2922 3796 4045 5773 5838
Size: 505 1080 189 1148 874 249 1728 65
Cuts at: 5838 6304 6958 7507 7591 7851
Size: 466 654 549 84 260
Fragments arranged by size:
1728 1148 1080 874 654 549 505 466
260 249 189 84 65
ApaLI G'TGCA_C
Cuts at: 0 542 1842 7851
Size: 542 1300 6009
Fragments arranged by size:
6009 1300 542
ApoI r'AATT_y
Cuts at: 0 1108 2838 3916 4443 4842 4860 7124 7539
Size: 1108 1730 1078 527 399 18 2264 415
Cuts at: 7539 7851
Size: 312
Fragments arranged by size:
2264 1730 1108 1078 527 415 399 312
18
AseI AT'TA_AT
Cuts at: 0 3542 3647 7851
Size: 3542 105 4204
Fragments arranged by size:
4204 3542 105
AvaI C'yCGr_G
Cuts at: 0 1436 3428 6174 6392 7769 7851
Size: 1436 1992 2746 218 1377 82
Fragments arranged by size:
2746 1992 1436 1377 218 82
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6108 6141 7851
Size: 6108 33 1710
Fragments arranged by size:
6108 1710 33
BamHI G'GATC_C
Cuts at: 0 119 414 6462 7851
Size: 119 295 6048 1389
Fragments arranged by size:
6048 1389 295 119
BanI G'GyrC_C
Cuts at: 0 25 271 1870 2142 5176 5389 6438 6663
Size: 25 246 1599 272 3034 213 1049 225
Cuts at: 6663 6801 7429 7679 7851
Size: 138 628 250 172
Fragments arranged by size:
3034 1599 1049 628 272 250 246 225
213 172 138 25
BanII G_rGCy'C
Cuts at: 0 3948 6183 7851
Size: 3948 2235 1668
Fragments arranged by size:
3948 2235 1668
BbsI GAAGACnn'nnnn_
Cuts at: 0 2625 5318 7851
Size: 2625 2693 2533
Fragments arranged by size:
2693 2625 2533
BbvCI CC'TCA_GC
Cuts at: 0 250 7451 7619 7851
Size: 250 7201 168 232
Fragments arranged by size:
7201 250 232 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5581 5615 7851
Size: 5581 34 2236
Fragments arranged by size:
5581 2236 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1405 3134 3360 7851
Size: 1405 1729 226 4491
Fragments arranged by size:
4491 1729 1405 226
BclI T'GATC_A
Cuts at: 0 1902 2913 5225 7851
Size: 1902 1011 2312 2626
Fragments arranged by size:
2626 2312 1902 1011
BfrBI ATG'CAT
Cuts at: 0 780 934 4169 5416 7275 7851
Size: 780 154 3235 1247 1859 576
Fragments arranged by size:
3235 1859 1247 780 576 154
BglI GCCn_nnn'nGGC
Cuts at: 0 3987 5575 7567 7851
Size: 3987 1588 1992 284
Fragments arranged by size:
3987 1992 1588 284
BglII A'GATC_T
Cuts at: 0 333 7851
Size: 333 7518
Bme1580I G_kGCm'C
Cuts at: 0 546 1846 3023 5181 7851
Size: 546 1300 1177 2158 2670
Fragments arranged by size:
2670 2158 1300 1177 546
BmrI ACTGGGnnnn_n'
Cuts at: 0 340 894 2374 2770 3563 5524 5619 5668
Size: 340 554 1480 396 793 1961 95 49
Cuts at: 5668 6462 7851
Size: 794 1389
Fragments arranged by size:
1961 1480 1389 794 793 554 396 340
95 49
BmtI G_CTAG'C
Cuts at: 0 1333 7851
Size: 1333 6518
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6078 7851
Size: 6078 1773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 2257 2431 2945 3512 3818 5402 5897
Size: 962 1295 174 514 567 306 1584 495
Cuts at: 5897 6167 7733 7829 7851
Size: 270 1566 96 22
Fragments arranged by size:
1584 1566 1295 962 567 514 495 306
270 174 96 22
Bpu10I CC'TnA_GC
Cuts at: 0 250 5300 5544 7451 7619 7851
Size: 250 5050 244 1907 168 232
Fragments arranged by size:
5050 1907 250 244 232 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1496 1978 2111 3855 6011 6494 7851
Size: 1271 225 482 133 1744 2156 483 1357
Fragments arranged by size:
2156 1744 1357 1271 483 482 225 133
BsaI GGTCTCn'nnnn_
Cuts at: 0 3655 5673 7851
Size: 3655 2018 2178
Fragments arranged by size:
3655 2178 2018
BsaAI yAC'GTr
Cuts at: 0 2149 7851
Size: 2149 5702
BsaBI GATnn'nnATC
Cuts at: 0 4546 7851
Size: 4546 3305
BsaHI Gr'CG_yC
Cuts at: 0 3399 7851
Size: 3399 4452
BsaWI w'CCGG_w
Cuts at: 0 515 4067 7851
Size: 515 3552 3784
Fragments arranged by size:
3784 3552 515
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 935 965 1334 1364 3485 3515 3830 3860
Size: 935 30 369 30 2121 30 315 30
Cuts at: 3860 5844 5874 5895 5925 7127 7157 7768 7798
Size: 1984 30 21 30 1202 30 611 30
Cuts at: 7798 7851
Size: 53
Fragments arranged by size:
2121 1984 1202 935 611 369 315 53
30 30 30 30 30 30 30 30
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 2353 2642 4489 5153 5958 6250 6890
Size: 52 2301 289 1847 664 805 292 640
Cuts at: 6890 7773 7826 7851
Size: 883 53 25
Fragments arranged by size:
2301 1847 883 805 664 640 292 289
53 52 25
BseYI C'CCAG_C
Cuts at: 0 72 76 3529 4615 6582 6615 6680 6824
Size: 72 4 3453 1086 1967 33 65 144
Cuts at: 6824 7562 7851
Size: 738 289
Fragments arranged by size:
3453 1967 1086 738 289 144 72 65
33 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1061 1103 1205 1870 1949 3209 4042
Size: 548 513 42 102 665 79 1260 833
Cuts at: 4042 4778 5957 6550 7851
Size: 736 1179 593 1301
Fragments arranged by size:
1301 1260 1179 833 736 665 593 548
513 102 79 42
BsiEI CG_ry'CG
Cuts at: 0 7654 7699 7851
Size: 7654 45 152
Fragments arranged by size:
7654 152 45
BsiHKAI G_wGCw'C
Cuts at: 0 546 971 1125 1199 1287 1846 2620 3221
Size: 546 425 154 74 88 559 774 601
Cuts at: 3221 5433 6183 7851
Size: 2212 750 1668
Fragments arranged by size:
2212 1668 774 750 601 559 546 425
154 88 74
BsmI GAATG_Cn'
Cuts at: 0 4171 4284 6413 7555 7851
Size: 4171 113 2129 1142 296
Fragments arranged by size:
4171 2129 1142 296 113
Bsp1286I G_dGCh'C
Cuts at: 0 546 971 1125 1199 1287 1846 2620 3023
Size: 546 425 154 74 88 559 774 403
Cuts at: 3023 3221 3948 5181 5433 6183 7851
Size: 198 727 1233 252 750 1668
Fragments arranged by size:
1668 1233 774 750 727 559 546 425
403 252 198 154 88 74
BspEI T'CCGG_A
Cuts at: 0 515 7851
Size: 515 7336
BspHI T'CATG_A
Cuts at: 0 879 3162 3198 7851
Size: 879 2283 36 4653
Fragments arranged by size:
4653 2283 879 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1033 3181 4030 4750 5352 6021 6312 7851
Size: 1033 2148 849 720 602 669 291 1539
Fragments arranged by size:
2148 1539 1033 849 720 669 602 291
BsrDI GCAATG_nn'
Cuts at: 0 442 3099 3221 3599 4993 7731 7851
Size: 442 2657 122 378 1394 2738 120
Fragments arranged by size:
2738 2657 1394 442 378 122 120
BssSI C'ACGA_G
Cuts at: 0 6704 7851
Size: 6704 1147
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1039 3232 4561 4799 7851
Size: 1039 2193 1329 238 3052
Fragments arranged by size:
3052 2193 1329 1039 238
BstEII G'GTnAC_C
Cuts at: 0 6851 7851
Size: 6851 1000
BstYI r'GATC_y
Cuts at: 0 119 333 414 501 5292 6462 7503 7851
Size: 119 214 81 87 4791 1170 1041 348
Fragments arranged by size:
4791 1170 1041 348 214 119 87 81
Bsu36I CC'TnA_GG
Cuts at: 0 506 2690 4436 7851
Size: 506 2184 1746 3415
Fragments arranged by size:
3415 2184 1746 506
BtgI C'CryG_G
Cuts at: 0 2171 5618 6110 6434 7851
Size: 2171 3447 492 324 1417
Fragments arranged by size:
3447 2171 1417 492 324
BtsI GCAGTG_nn'
Cuts at: 0 288 917 1217 4824 4879 5153 6533 7851
Size: 288 629 300 3607 55 274 1380 1318
Fragments arranged by size:
3607 1380 1318 629 300 288 274 55
ClaI AT'CG_AT
Cuts at: 0 3109 7851
Size: 3109 4742
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3077 3112 4524 4559 7028 7063 7851
Size: 3077 35 1412 35 2469 35 788
Fragments arranged by size:
3077 2469 1412 788 35 35 35
DraI TTT'AAA
Cuts at: 0 2712 4295 7851
Size: 2712 1583 3556
Fragments arranged by size:
3556 2712 1583
DraIII CAC_nnn'GTG
Cuts at: 0 3056 7851
Size: 3056 4795
EaeI y'GGCC_r
Cuts at: 0 2034 7651 7851
Size: 2034 5617 200
Fragments arranged by size:
5617 2034 200
EagI C'GGCC_G
Cuts at: 0 7651 7851
Size: 7651 200
EarI CTCTTCn'nnn_
Cuts at: 0 2468 3813 4319 4466 5130 5817 6897 7633
Size: 2468 1345 506 147 664 687 1080 736
Cuts at: 7633 7833 7851
Size: 200 18
Fragments arranged by size:
2468 1345 1080 736 687 664 506 200
147 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 57 86 221 7851
Size: 57 29 135 7630
Fragments arranged by size:
7630 135 57 29
EcoICRI GAG'CTC
Cuts at: 0 6181 7851
Size: 6181 1670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1574 1814 2257 2366 2431 2836 2945
Size: 962 612 240 443 109 65 405 109
Cuts at: 2945 3113 3512 3818 5402 5897 6167 6524 6621
Size: 168 399 306 1584 495 270 357 97
Cuts at: 6621 6795 7568 7733 7829 7838 7851
Size: 174 773 165 96 9 13
Fragments arranged by size:
1584 962 773 612 495 443 405 399
357 306 270 240 174 168 165 109
109 97 96 65 13 9
EcoO109I rG'GnC_Cy
Cuts at: 0 42 80 2289 7474 7851
Size: 42 38 2209 5185 377
Fragments arranged by size:
5185 2209 377 42 38
EcoRI G'AATT_C
Cuts at: 0 1108 4443 4860 7124 7851
Size: 1108 3335 417 2264 727
Fragments arranged by size:
3335 2264 1108 727 417
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 787 988 1826 2416 6052 7851
Size: 787 201 838 590 3636 1799
Fragments arranged by size:
3636 1799 838 787 590 201
HaeII r_GCGC'y
Cuts at: 0 223 1676 7016 7851
Size: 223 1453 5340 835
Fragments arranged by size:
5340 1453 835 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 283 295 315 327 880 912
Size: 34 32 217 12 20 12 553 32
Cuts at: 912 2584 2616 2725 2757 2826 2858 5135 5167
Size: 1672 32 109 32 69 32 2277 32
Cuts at: 5167 5589 5621 5895 5927 6076 6108 6454 6486
Size: 422 32 274 32 149 32 346 32
Cuts at: 6486 7851
Size: 1365
Fragments arranged by size:
2277 1672 1365 553 422 346 274 217
149 109 69 34 32 32 32 32
32 32 32 32 32 32 20 12
12
HincII GTy'rAC
Cuts at: 0 476 894 954 1881 2697 3135 4973 7851
Size: 476 418 60 927 816 438 1838 2878
Fragments arranged by size:
2878 1838 927 816 476 438 418 60
HindIII A'AGCT_T
Cuts at: 0 491 632 7851
Size: 491 141 7219
Fragments arranged by size:
7219 491 141
HpaI GTT'AAC
Cuts at: 0 1881 7851
Size: 1881 5970
KpnI G_GTAC'C
Cuts at: 0 5393 6442 7851
Size: 5393 1049 1409
Fragments arranged by size:
5393 1409 1049
MfeI C'AATT_G
Cuts at: 0 7100 7851
Size: 7100 751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2042 3056 3282 3795 5458 5530 7164 7811
Size: 2042 1014 226 513 1663 72 1634 647
Cuts at: 7811 7851
Size: 40
Fragments arranged by size:
2042 1663 1634 1014 647 513 226 72
40
MscI TGG'CCA
Cuts at: 0 2036 7851
Size: 2036 5815
MslI CAynn'nnrTG
Cuts at: 0 785 1103 1742 2042 2101 3384 3953 4013
Size: 785 318 639 300 59 1283 569 60
Cuts at: 4013 4223 4576 5186 7851
Size: 210 353 610 2665
Fragments arranged by size:
2665 1283 785 639 610 569 353 318
300 210 60 59
MspA1I CmG'CkG
Cuts at: 0 76 176 191 197 3004 6523 6544 7851
Size: 76 100 15 6 2807 3519 21 1307
Fragments arranged by size:
3519 2807 1307 100 76 21 15 6
NcoI C'CATG_G
Cuts at: 0 2171 5618 6110 6434 7851
Size: 2171 3447 492 324 1417
Fragments arranged by size:
3447 2171 1417 492 324
NdeI CA'TA_TG
Cuts at: 0 4694 7851
Size: 4694 3157
NheI G'CTAG_C
Cuts at: 0 1329 7851
Size: 1329 6522
NsiI A_TGCA'T
Cuts at: 0 782 936 4171 5418 7277 7851
Size: 782 154 3235 1247 1859 574
Fragments arranged by size:
3235 1859 1247 782 574 154
NspI r_CATG'y
Cuts at: 0 784 938 1252 1299 1351 1687 1928 3301
Size: 784 154 314 47 52 336 241 1373
Cuts at: 3301 3389 3607 6783 7851
Size: 88 218 3176 1068
Fragments arranged by size:
3176 1373 1068 784 336 314 241 218
154 88 52 47
PciI A'CATG_T
Cuts at: 0 1248 1347 3603 7851
Size: 1248 99 2256 4248
Fragments arranged by size:
4248 2256 1248 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 2322 6111 7851
Size: 2322 3789 1740
Fragments arranged by size:
3789 2322 1740
PfoI T'CCnGG_A
Cuts at: 0 7467 7851
Size: 7467 384
PmlI CAC'GTG
Cuts at: 0 2149 7851
Size: 2149 5702
PpuMI rG'GwC_Cy
Cuts at: 0 42 2289 7851
Size: 42 2247 5562
Fragments arranged by size:
5562 2247 42
PshAI GACnn'nnGTC
Cuts at: 0 3905 7851
Size: 3905 3946
PspXI vC'TCGA_Gb
Cuts at: 0 3428 6392 7851
Size: 3428 2964 1459
Fragments arranged by size:
3428 2964 1459
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3351 3383 4103 4135 7851
Size: 3351 32 720 32 3716
Fragments arranged by size:
3716 3351 720 32 32
PstI C_TGCA'G
Cuts at: 0 390 1305 2516 3615 4266 6528 7851
Size: 390 915 1211 1099 651 2262 1323
Fragments arranged by size:
2262 1323 1211 1099 915 651 390
PvuII CAG'CTG
Cuts at: 0 76 3004 6523 6544 7851
Size: 76 2928 3519 21 1307
Fragments arranged by size:
3519 2928 1307 76 21
SacI G_AGCT'C
Cuts at: 0 6183 7851
Size: 6183 1668
SapI GCTCTTCn'nnn_
Cuts at: 0 2468 7851
Size: 2468 5383
ScaI AGT'ACT
Cuts at: 0 384 4914 6021 7851
Size: 384 4530 1107 1830
Fragments arranged by size:
4530 1830 1107 384
SfcI C'TryA_G
Cuts at: 0 386 1301 1889 2512 3611 4262 4664 5816
Size: 386 915 588 623 1099 651 402 1152
Cuts at: 5816 6524 6713 7851
Size: 708 189 1138
Fragments arranged by size:
1152 1138 1099 915 708 651 623 588
402 386 189
SmaI CCC'GGG
Cuts at: 0 1438 6176 7771 7851
Size: 1438 4738 1595 80
Fragments arranged by size:
4738 1595 1438 80
SmlI C'TyrA_G
Cuts at: 0 1286 1511 1993 2126 2401 3428 3870 6026
Size: 1286 225 482 133 275 1027 442 2156
Cuts at: 6026 6392 6509 7851
Size: 366 117 1342
Fragments arranged by size:
2156 1342 1286 1027 482 442 366 275
225 133 117
SpeI A'CTAG_T
Cuts at: 0 6838 7851
Size: 6838 1013
SphI G_CATG'C
Cuts at: 0 784 1687 7851
Size: 784 903 6164
Fragments arranged by size:
6164 903 784
SspI AAT'ATT
Cuts at: 0 1657 2501 4126 7851
Size: 1657 844 1625 3725
Fragments arranged by size:
3725 1657 1625 844
StuI AGG'CCT
Cuts at: 0 1234 7851
Size: 1234 6617
StyI C'CwwG_G
Cuts at: 0 2171 5618 6110 6434 7003 7851
Size: 2171 3447 492 324 569 848
Fragments arranged by size:
3447 2171 848 569 492 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 2324 3749 5416 6090 6209 6342 7647
Size: 100 2224 1425 1667 674 119 133 1305
Cuts at: 7647 7713 7744 7851
Size: 66 31 107
Fragments arranged by size:
2224 1667 1425 1305 674 133 119 107
100 66 31
TatI w'GTAC_w
Cuts at: 0 382 4912 6019 7851
Size: 382 4530 1107 1832
Fragments arranged by size:
4530 1832 1107 382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 246 278 399 431 3260 3292 7768 7800
Size: 246 32 121 32 2829 32 4476 32
Cuts at: 7800 7851
Size: 51
Fragments arranged by size:
4476 2829 246 121 51 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 4989 7851
Size: 4989 2862
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1614 2568 7508 7851
Size: 1614 954 4940 343
Fragments arranged by size:
4940 1614 954 343
XhoI C'TCGA_G
Cuts at: 0 3428 6392 7851
Size: 3428 2964 1459
Fragments arranged by size:
3428 2964 1459
XmaI C'CCGG_G
Cuts at: 0 1436 6174 7769 7851
Size: 1436 4738 1595 82
Fragments arranged by size:
4738 1595 1436 82
ZraI GAC'GTC
Cuts at: 0 3400 7851
Size: 3400 4451
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BssSI BstAPI BstEII BstYI
Bsu36I BtgI BtsI ClaI CspCI DraI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI
MscI MslI MspA1I NcoI NdeI NheI NsiI NspI
PciI PflMI PfoI PmlI PpuMI PshAI PspXI PsrI
PstI PvuII SacI SapI ScaI SfcI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AgeI AloI ApaI AscI AsiSI AvrII BbeI
BlpI BmgBI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII
BstBI BstXI BstZ17I BtgZI DrdI EcoNI EcoRV FseI
FspI FspAI KasI MluI NaeI NarI NgoMIV NotI
NruI PacI PasI PmeI PpiI PsiI PspOMI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SrfI SwaI XbaI XmnI