Restriction map for
pFN21AE5837
(Linear) (Six-base) MAPSORT of: KIEE5837.seq Check: 4157 from: 1 to: 5046
KIEE5837.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1023 1941 2662 2853 4512 5046
Size: 1023 918 721 191 1659 534
Fragments arranged by size:
1659 1023 918 721 534 191
AccI GT'mk_AC
Cuts at: 0 218 4025 5046
Size: 218 3807 1021
Fragments arranged by size:
3807 1021 218
Acc65I G'GTAC_C
Cuts at: 0 51 5046
Size: 51 4995
AclI AA'CG_TT
Cuts at: 0 1069 1316 5046
Size: 1069 247 3730
Fragments arranged by size:
3730 1069 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 632 962 2600 3491 3818 4285 4410
Size: 589 43 330 1638 891 327 467 125
Cuts at: 4410 4635 4942 5016 5044 5046
Size: 225 307 74 28 2
Fragments arranged by size:
1638 891 589 467 330 327 307 225
125 74 43 28 2
AflIII A'CryG_T
Cuts at: 0 60 221 1607 2620 5046
Size: 60 161 1386 1013 2426
Fragments arranged by size:
2426 1386 1013 161 60
AgeI A'CCGG_T
Cuts at: 0 4904 5046
Size: 4904 142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2258 5046
Size: 2258 2788
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2418 2450 5046
Size: 2418 32 2596
Fragments arranged by size:
2596 2418 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1966 2125 2469 2773 4972 5022 5046
Size: 1966 159 344 304 2199 50 24
Fragments arranged by size:
2199 1966 344 304 159 50 24
ApaLI G'TGCA_C
Cuts at: 0 3654 5046
Size: 3654 1392
ApoI r'AATT_y
Cuts at: 0 163 243 333 906 1374 1404 1718 2590
Size: 163 80 90 573 468 30 314 872
Cuts at: 2590 3493 3595 3954 5046
Size: 903 102 359 1092
Fragments arranged by size:
1092 903 872 573 468 359 314 163
102 90 80 30
AvaI C'yCGr_G
Cuts at: 0 3136 5046
Size: 3136 1910
BanI G'GyrC_C
Cuts at: 0 51 702 1229 5046
Size: 51 651 527 3817
Fragments arranged by size:
3817 651 527 51
BanII G_rGCy'C
Cuts at: 0 407 493 1571 1930 2389 2737 2791 3116
Size: 407 86 1078 359 459 348 54 325
Cuts at: 3116 3347 4226 4608 5046
Size: 231 879 382 438
Fragments arranged by size:
1078 879 459 438 407 382 359 348
325 231 86 54
BbeI G_GCGC'C
Cuts at: 0 1233 5046
Size: 1233 3813
BbsI GAAGACnn'nnnn_
Cuts at: 0 434 577 638 875 1001 1990 5046
Size: 434 143 61 237 126 989 3056
Fragments arranged by size:
3056 989 434 237 143 126 61
BbvCI CC'TCA_GC
Cuts at: 0 47 654 2501 5046
Size: 47 607 1847 2545
Fragments arranged by size:
2545 1847 607 47
BclI T'GATC_A
Cuts at: 0 1367 5046
Size: 1367 3679
BfrBI ATG'CAT
Cuts at: 0 1137 4261 5046
Size: 1137 3124 785
Fragments arranged by size:
3124 1137 785
BglI GCCn_nnn'nGGC
Cuts at: 0 4203 5046
Size: 4203 843
BglII A'GATC_T
Cuts at: 0 196 1866 2544 3441 3633 5046
Size: 196 1670 678 897 192 1413
Fragments arranged by size:
1670 1413 897 678 196 192
BlpI GC'TnA_GC
Cuts at: 0 2762 4225 4802 5046
Size: 2762 1463 577 244
Fragments arranged by size:
2762 1463 577 244
Bme1580I G_kGCm'C
Cuts at: 0 3658 5046
Size: 3658 1388
BmrI ACTGGGnnnn_n'
Cuts at: 0 1340 2216 3342 4102 5046
Size: 1340 876 1126 760 944
Fragments arranged by size:
1340 1126 944 876 760
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4374 5046
Size: 4374 672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 692 1328 1562 1793 2504 3292 4710
Size: 664 28 636 234 231 711 788 1418
Cuts at: 4710 5046
Size: 336
Fragments arranged by size:
1418 788 711 664 636 336 234 231
28
Bpu10I CC'TnA_GC
Cuts at: 0 47 318 654 2501 4614 5046
Size: 47 271 336 1847 2113 432
Fragments arranged by size:
2113 1847 432 336 271 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 1144 2172 5001 5046
Size: 682 462 1028 2829 45
Fragments arranged by size:
2829 1028 682 462 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 5046
Size: 31 5015
BsaAI yAC'GTr
Cuts at: 0 2621 4836 5046
Size: 2621 2215 210
Fragments arranged by size:
2621 2215 210
BsaHI Gr'CG_yC
Cuts at: 0 1230 5046
Size: 1230 3816
BsaWI w'CCGG_w
Cuts at: 0 78 2872 4904 5046
Size: 78 2794 2032 142
Fragments arranged by size:
2794 2032 142 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1301 1331 2199 2229 2724 2754 5046
Size: 1301 30 868 30 495 30 2292
Fragments arranged by size:
2292 1301 868 495 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 820 823 826 835 838 1648 2207
Size: 415 405 3 3 9 3 810 559
Cuts at: 2207 2876 3153 3168 3171 3198 3218 3793 4442
Size: 669 277 15 3 27 20 575 649
Cuts at: 4442 4632 4698 4701 5046
Size: 190 66 3 345
Fragments arranged by size:
810 669 649 575 559 415 405 345
277 190 66 27 20 15 9 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1258 1571 3709 5046
Size: 1258 313 2138 1337
Fragments arranged by size:
2138 1337 1258 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1919 2632 3523 3987 4816 5046
Size: 1919 713 891 464 829 230
Fragments arranged by size:
1919 891 829 713 464 230
BsiHKAI G_wGCw'C
Cuts at: 0 407 493 3658 4226 5046
Size: 407 86 3165 568 820
Fragments arranged by size:
3165 820 568 407 86
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1488 5046
Size: 1488 3558
Bsp1286I G_dGCh'C
Cuts at: 0 407 493 1571 1930 2389 2737 2791 3116
Size: 407 86 1078 359 459 348 54 325
Cuts at: 3116 3347 3658 4226 4608 5046
Size: 231 311 568 382 438
Fragments arranged by size:
1078 568 459 438 407 382 359 348
325 311 231 86 54
BspEI T'CCGG_A
Cuts at: 0 78 5046
Size: 78 4968
BspHI T'CATG_A
Cuts at: 0 1370 5046
Size: 1370 3676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1023 1941 2662 2853 4512 5046
Size: 1023 918 721 191 1659 534
Fragments arranged by size:
1659 1023 918 721 534 191
BsrBI CCG'CTC
Cuts at: 0 1227 4929 5046
Size: 1227 3702 117
Fragments arranged by size:
3702 1227 117
BsrFI r'CCGG_y
Cuts at: 0 669 1211 4904 5046
Size: 669 542 3693 142
Fragments arranged by size:
3693 669 542 142
BsrGI T'GTAC_A
Cuts at: 0 1658 5046
Size: 1658 3388
BssSI C'ACGA_G
Cuts at: 0 3664 5046
Size: 3664 1382
BstAPI GCAn_nnn'nTGC
Cuts at: 0 724 2453 3741 4578 4972 5046
Size: 724 1729 1288 837 394 74
Fragments arranged by size:
1729 1288 837 724 394 74
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2528 5046
Size: 2528 2518
BstYI r'GATC_y
Cuts at: 0 196 1284 1866 2544 2769 2904 3441 3633
Size: 196 1088 582 678 225 135 537 192
Cuts at: 3633 3705 3750 3849 5046
Size: 72 45 99 1197
Fragments arranged by size:
1197 1088 678 582 537 225 196 192
135 99 72 45
BstZ17I GTA'TAC
Cuts at: 0 219 5046
Size: 219 4827
BtgI C'CryG_G
Cuts at: 0 1973 3107 3929 5046
Size: 1973 1134 822 1117
Fragments arranged by size:
1973 1134 1117 822
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3523 5046
Size: 3523 1523
BtsI GCAGTG_nn'
Cuts at: 0 1936 2033 2454 5046
Size: 1936 97 421 2592
Fragments arranged by size:
2592 1936 421 97
ClaI AT'CG_AT
Cuts at: 0 2696 5046
Size: 2696 2350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1164 1199 5046
Size: 1164 35 3847
Fragments arranged by size:
3847 1164 35
DraI TTT'AAA
Cuts at: 0 152 380 3894 5046
Size: 152 228 3514 1152
Fragments arranged by size:
3514 1152 228 152
DraIII CAC_nnn'GTG
Cuts at: 0 4010 5046
Size: 4010 1036
DrdI GACnn_nn'nnGTC
Cuts at: 0 1975 5046
Size: 1975 3071
EaeI y'GGCC_r
Cuts at: 0 29 671 689 4742 4784 5046
Size: 29 642 18 4053 42 262
Fragments arranged by size:
4053 642 262 42 29 18
EarI CTCTTCn'nnn_
Cuts at: 0 320 455 632 797 812 1037 1415 1559
Size: 320 135 177 165 15 225 378 144
Cuts at: 1559 2916 2987 3000 3171 3189 3216 3335 3770
Size: 1357 71 13 171 18 27 119 435
Cuts at: 3770 3794 4078 5046
Size: 24 284 968
Fragments arranged by size:
1357 968 435 378 320 284 225 177
171 165 144 135 119 71 27 24
18 15 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2220 2942 5046
Size: 2220 722 2104
Fragments arranged by size:
2220 2104 722
EcoICRI GAG'CTC
Cuts at: 0 405 491 4224 5046
Size: 405 86 3733 822
Fragments arranged by size:
3733 822 405 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 632 664 692 962 1328 1562 1793
Size: 589 43 32 28 270 366 234 231
Cuts at: 1793 2504 2600 3292 3491 3818 4285 4410 4635
Size: 711 96 692 199 327 467 125 225
Cuts at: 4635 4710 4942 5016 5044 5046
Size: 75 232 74 28 2
Fragments arranged by size:
711 692 589 467 366 327 270 234
232 231 225 199 125 96 75 74
43 32 28 28 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 682 2190 4716 5046
Size: 46 636 1508 2526 330
Fragments arranged by size:
2526 1508 636 330 46
EcoO109I rG'GnC_Cy
Cuts at: 0 622 1851 2143 3689 4618 5046
Size: 622 1229 292 1546 929 428
Fragments arranged by size:
1546 1229 929 622 428 292
EcoRI G'AATT_C
Cuts at: 0 1374 2590 5046
Size: 1374 1216 2456
Fragments arranged by size:
2456 1374 1216
EcoRV GAT'ATC
Cuts at: 0 3293 5046
Size: 3293 1753
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 109 1006 1576 4315 4350 4651 5046
Size: 109 897 570 2739 35 301 395
Fragments arranged by size:
2739 897 570 395 301 109 35
HaeII r_GCGC'y
Cuts at: 0 476 1233 5012 5046
Size: 476 757 3779 34
Fragments arranged by size:
3779 757 476 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 1485 1517 1750 1782 2014 2046
Size: 577 32 876 32 233 32 232 32
Cuts at: 2046 2199 2231 2724 2756 3334 3366 3834 3854
Size: 153 32 493 32 578 32 468 20
Cuts at: 3854 3866 3886 5046
Size: 12 20 1160
Fragments arranged by size:
1160 876 578 577 493 468 233 232
153 32 32 32 32 32 32 32
20 20 12
HincII GTy'rAC
Cuts at: 0 111 1087 4026 5046
Size: 111 976 2939 1020
Fragments arranged by size:
2939 1020 976 111
HindIII A'AGCT_T
Cuts at: 0 3888 5046
Size: 3888 1158
KasI G'GCGC_C
Cuts at: 0 1229 5046
Size: 1229 3817
KpnI G_GTAC'C
Cuts at: 0 55 5046
Size: 55 4991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 3169 4206 5046
Size: 845 2324 1037 840
Fragments arranged by size:
2324 1037 845 840
MscI TGG'CCA
Cuts at: 0 691 4744 4786 5046
Size: 691 4053 42 260
Fragments arranged by size:
4053 691 260 42
MslI CAynn'nnrTG
Cuts at: 0 2346 2570 2656 3015 5046
Size: 2346 224 86 359 2031
Fragments arranged by size:
2346 2031 359 224 86
MspA1I CmG'CkG
Cuts at: 0 448 2483 2880 4430 4552 4962 4969 5046
Size: 448 2035 397 1550 122 410 7 77
Fragments arranged by size:
2035 1550 448 410 397 122 77 7
NaeI GCC'GGC
Cuts at: 0 671 5046
Size: 671 4375
NarI GG'CG_CC
Cuts at: 0 1230 5046
Size: 1230 3816
NcoI C'CATG_G
Cuts at: 0 1973 3929 5046
Size: 1973 1956 1117
Fragments arranged by size:
1973 1956 1117
NdeI CA'TA_TG
Cuts at: 0 3731 5046
Size: 3731 1315
NgoMIV G'CCGG_C
Cuts at: 0 669 5046
Size: 669 4377
NsiI A_TGCA'T
Cuts at: 0 1139 4263 5046
Size: 1139 3124 783
Fragments arranged by size:
3124 1139 783
NspI r_CATG'y
Cuts at: 0 64 225 897 1137 2661 4581 5046
Size: 64 161 672 240 1524 1920 465
Fragments arranged by size:
1920 1524 672 465 240 161 64
PasI CC'CwG_GG
Cuts at: 0 3335 5046
Size: 3335 1711
PciI A'CATG_T
Cuts at: 0 60 221 5046
Size: 60 161 4825
Fragments arranged by size:
4825 161 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 178 687 2272 5046
Size: 178 509 1585 2774
Fragments arranged by size:
2774 1585 509 178
PfoI T'CCnGG_A
Cuts at: 0 680 5046
Size: 680 4366
PmlI CAC'GTG
Cuts at: 0 4836 5046
Size: 4836 210
PpuMI rG'GwC_Cy
Cuts at: 0 622 1851 2143 5046
Size: 622 1229 292 2903
Fragments arranged by size:
2903 1229 622 292
PshAI GACnn'nnGTC
Cuts at: 0 2521 2780 5046
Size: 2521 259 2266
Fragments arranged by size:
2521 2266 259
PstI C_TGCA'G
Cuts at: 0 618 2112 2666 2721 2855 5046
Size: 618 1494 554 55 134 2191
Fragments arranged by size:
2191 1494 618 554 134 55
PvuII CAG'CTG
Cuts at: 0 448 2880 4430 4962 4969 5046
Size: 448 2432 1550 532 7 77
Fragments arranged by size:
2432 1550 532 448 77 7
RsrII CG'GwC_CG
Cuts at: 0 2782 5046
Size: 2782 2264
SacI G_AGCT'C
Cuts at: 0 407 493 4226 5046
Size: 407 86 3733 820
Fragments arranged by size:
3733 820 407 86
SalI G'TCGA_C
Cuts at: 0 4024 5046
Size: 4024 1022
SapI GCTCTTCn'nnn_
Cuts at: 0 320 455 1559 3335 3794 5046
Size: 320 135 1104 1776 459 1252
Fragments arranged by size:
1776 1252 1104 459 320 135
SbfI CC_TGCA'GG
Cuts at: 0 2112 5046
Size: 2112 2934
ScaI AGT'ACT
Cuts at: 0 1180 5046
Size: 1180 3866
SfcI C'TryA_G
Cuts at: 0 614 1270 2108 2662 2705 2717 2851 5046
Size: 614 656 838 554 43 12 134 2195
Fragments arranged by size:
2195 838 656 614 554 134 43 12
SfoI GGC'GCC
Cuts at: 0 1231 5046
Size: 1231 3815
SmaI CCC'GGG
Cuts at: 0 3138 5046
Size: 3138 1908
SmlI C'TyrA_G
Cuts at: 0 661 1123 2187 4980 5046
Size: 661 462 1064 2793 66
Fragments arranged by size:
2793 1064 661 462 66
SpeI A'CTAG_T
Cuts at: 0 1738 5046
Size: 1738 3308
SphI G_CATG'C
Cuts at: 0 897 2661 4581 5046
Size: 897 1764 1920 465
Fragments arranged by size:
1920 1764 897 465
SspI AAT'ATT
Cuts at: 0 1459 3972 5046
Size: 1459 2513 1074
Fragments arranged by size:
2513 1459 1074
StuI AGG'CCT
Cuts at: 0 1559 5046
Size: 1559 3487
StyI C'CwwG_G
Cuts at: 0 1973 3929 4880 5046
Size: 1973 1956 951 166
Fragments arranged by size:
1973 1956 951 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1615 2305 2770 3029 3656 4639 5046
Size: 1615 690 465 259 627 983 407
Fragments arranged by size:
1615 983 690 627 465 407 259
TatI w'GTAC_w
Cuts at: 0 1178 1658 4284 5046
Size: 1178 480 2626 762
Fragments arranged by size:
2626 1178 762 480
XbaI T'CTAG_A
Cuts at: 0 4921 5046
Size: 4921 125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 248 3105 4143 5046
Size: 39 209 2857 1038 903
Fragments arranged by size:
2857 1038 903 209 39
XmaI C'CCGG_G
Cuts at: 0 3136 5046
Size: 3136 1910
XmnI GAAnn'nnTTC
Cuts at: 0 1868 5029 5046
Size: 1868 3161 17
Fragments arranged by size:
3161 1868 17
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmlI PpuMI PshAI PstI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BmgBI BmtI
BsaBI BsiEI BsiWI BsmI BsrDI BssHII BstBI BstEII
Bsu36I EagI FseI FspI FspAI HpaI MfeI MluI
NheI NotI NruI PacI PmeI PpiI PsiI PspOMI
PspXI PsrI PvuI SacII SanDI SexAI SfiI SgfI
SgrAI SnaBI SrfI SwaI TstI Tth111I XhoI ZraI