Restriction map for
pFN21AE5827
(Linear) (Six-base) MAPSORT of: KIEE5827.seq Check: 9382 from: 1 to: 2751
KIEE5827.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1704 2751
Size: 1704 1047
AatII G_ACGT'C
Cuts at: 0 983 2751
Size: 983 1768
AccI GT'mk_AC
Cuts at: 0 340 2751
Size: 340 2411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1424 2253 2751
Size: 1016 408 829 498
Fragments arranged by size:
1016 829 498 408
AfeI AGC'GCT
Cuts at: 0 478 2751
Size: 478 2273
AhdI GACnn_n'nnGTC
Cuts at: 0 354 2751
Size: 354 2397
AleI CACnn'nnGTG
Cuts at: 0 487 2751
Size: 487 2264
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1462 2014 2751
Size: 1462 552 737
Fragments arranged by size:
1462 737 552
AlwNI CAG_nnn'CTG
Cuts at: 0 168 1684 2751
Size: 168 1516 1067
Fragments arranged by size:
1516 1067 168
ApaI G_GGCC'C
Cuts at: 0 2018 2751
Size: 2018 733
ApoI r'AATT_y
Cuts at: 0 1045 2751
Size: 1045 1706
AvaI C'yCGr_G
Cuts at: 0 63 1580 1782 2009 2751
Size: 63 1517 202 227 742
Fragments arranged by size:
1517 742 227 202 63
BamHI G'GATC_C
Cuts at: 0 1408 2751
Size: 1408 1343
BanI G'GyrC_C
Cuts at: 0 778 1133 1255 1970 2062 2751
Size: 778 355 122 715 92 689
Fragments arranged by size:
778 715 689 355 122 92
BanII G_rGCy'C
Cuts at: 0 20 1286 1663 2009 2018 2298 2666 2751
Size: 20 1266 377 346 9 280 368 85
Fragments arranged by size:
1266 377 368 346 280 85 20 9
BbeI G_GCGC'C
Cuts at: 0 1974 2066 2751
Size: 1974 92 685
Fragments arranged by size:
1974 685 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 703 1043 2751
Size: 703 340 1708
Fragments arranged by size:
1708 703 340
BbvCI CC'TCA_GC
Cuts at: 0 1931 2751
Size: 1931 820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1893 1927 2000 2034 2751
Size: 1893 34 73 34 717
Fragments arranged by size:
1893 717 73 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 612 2751
Size: 612 2139
BglI GCCn_nnn'nGGC
Cuts at: 0 2013 2751
Size: 2013 738
BglII A'GATC_T
Cuts at: 0 837 1602 2528 2751
Size: 837 765 926 223
Fragments arranged by size:
926 837 765 223
Bme1580I G_kGCm'C
Cuts at: 0 2018 2751
Size: 2018 733
BmrI ACTGGGnnnn_n'
Cuts at: 0 245 641 1694 2751
Size: 245 396 1053 1057
Fragments arranged by size:
1057 1053 396 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 337 988 1292 1400 1454 2312 2476
Size: 92 245 651 304 108 54 858 164
Cuts at: 2476 2751
Size: 275
Fragments arranged by size:
858 651 304 275 245 164 108 92
54
Bpu10I CC'TnA_GC
Cuts at: 0 410 1931 2751
Size: 410 1521 820
Fragments arranged by size:
1521 820 410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 753 2086 2344 2751
Size: 753 1333 258 407
Fragments arranged by size:
1333 753 407 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 1626 2751
Size: 1626 1125
BsaBI GATnn'nnATC
Cuts at: 0 58 2751
Size: 58 2693
BsaHI Gr'CG_yC
Cuts at: 0 980 1971 2063 2751
Size: 980 991 92 688
Fragments arranged by size:
991 980 688 92
BsaWI w'CCGG_w
Cuts at: 0 239 2751
Size: 239 2512
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 335 365 633 663 2194 2224 2449 2479
Size: 335 30 268 30 1531 30 225 30
Cuts at: 2479 2563 2593 2751
Size: 84 30 158
Fragments arranged by size:
1531 335 268 225 158 84 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 889 1807 1828 1837 1855 2461 2751
Size: 889 918 21 9 18 606 290
Fragments arranged by size:
918 889 606 290 21 18 9
BseYI C'CCAG_C
Cuts at: 0 470 1273 1327 1363 1435 2092 2112 2259
Size: 470 803 54 36 72 657 20 147
Cuts at: 2259 2751
Size: 492
Fragments arranged by size:
803 657 492 470 147 72 54 36
20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 506 724 1975 2751
Size: 369 137 218 1251 776
Fragments arranged by size:
1251 776 369 218 137
BsiHKAI G_wGCw'C
Cuts at: 0 34 209 1058 1663 2597 2751
Size: 34 175 849 605 934 154
Fragments arranged by size:
934 849 605 175 154 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1195 1909 2751
Size: 1195 714 842
Fragments arranged by size:
1195 842 714
Bsp1286I G_dGCh'C
Cuts at: 0 20 34 209 1058 1286 1663 2009 2018
Size: 20 14 175 849 228 377 346 9
Cuts at: 2018 2298 2597 2666 2751
Size: 280 299 69 85
Fragments arranged by size:
849 377 346 299 280 228 175 85
69 20 14 9
BspEI T'CCGG_A
Cuts at: 0 239 2751
Size: 239 2512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 947 1512 1704 2618 2751
Size: 947 565 192 914 133
Fragments arranged by size:
947 914 565 192 133
BsrBI CCG'CTC
Cuts at: 0 1360 2177 2751
Size: 1360 817 574
Fragments arranged by size:
1360 817 574
BsrDI GCAATG_nn'
Cuts at: 0 2127 2751
Size: 2127 624
BssSI C'ACGA_G
Cuts at: 0 232 268 2751
Size: 232 36 2483
Fragments arranged by size:
2483 232 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 29 516 2751
Size: 29 487 2235
Fragments arranged by size:
2235 487 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 807 2751
Size: 807 1944
BstYI r'GATC_y
Cuts at: 0 236 837 1408 1602 2264 2528 2751
Size: 236 601 571 194 662 264 223
Fragments arranged by size:
662 601 571 264 236 223 194
Bsu36I CC'TnA_GG
Cuts at: 0 985 1727 2751
Size: 985 742 1024
Fragments arranged by size:
1024 985 742
BtgI C'CryG_G
Cuts at: 0 125 390 720 2303 2751
Size: 125 265 330 1583 448
Fragments arranged by size:
1583 448 330 265 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1134 1880 2529 2751
Size: 1134 746 649 222
Fragments arranged by size:
1134 746 649 222
BtsI GCAGTG_nn'
Cuts at: 0 536 1893 1945 2043 2417 2751
Size: 536 1357 52 98 374 334
Fragments arranged by size:
1357 536 374 334 98 52
DraIII CAC_nnn'GTG
Cuts at: 0 1951 2751
Size: 1951 800
EaeI y'GGCC_r
Cuts at: 0 491 969 1293 2751
Size: 491 478 324 1458
Fragments arranged by size:
1458 491 478 324
EarI CTCTTCn'nnn_
Cuts at: 0 952 1114 1213 1799 2155 2212 2751
Size: 952 162 99 586 356 57 539
Fragments arranged by size:
952 586 539 356 162 99 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 160 1421 2292 2751
Size: 160 1261 871 459
Fragments arranged by size:
1261 871 459 160
EcoICRI GAG'CTC
Cuts at: 0 1661 2751
Size: 1661 1090
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 337 988 1016 1292 1400 1424 1454
Size: 92 245 651 28 276 108 24 30
Cuts at: 1454 2253 2312 2476 2751
Size: 799 59 164 275
Fragments arranged by size:
799 651 276 275 245 164 108 92
59 30 28 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1645 1999 2239 2751
Size: 1645 354 240 512
Fragments arranged by size:
1645 512 354 240
EcoO109I rG'GnC_Cy
Cuts at: 0 315 1249 1497 1533 1584 1638 1668 1689
Size: 315 934 248 36 51 54 30 21
Cuts at: 1689 2014 2015 2751
Size: 325 1 736
Fragments arranged by size:
934 736 325 315 248 54 51 36
30 21 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 96 2443 2547 2673 2751
Size: 96 2347 104 126 78
Fragments arranged by size:
2347 126 104 96 78
HaeII r_GCGC'y
Cuts at: 0 42 420 480 1974 2066 2751
Size: 42 378 60 1494 92 685
Fragments arranged by size:
1494 685 378 92 60 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 2194 2226 2751
Size: 340 32 1822 32 525
Fragments arranged by size:
1822 525 340 32 32
HincII GTy'rAC
Cuts at: 0 341 2571 2751
Size: 341 2230 180
Fragments arranged by size:
2230 341 180
KasI G'GCGC_C
Cuts at: 0 1970 2062 2751
Size: 1970 92 689
Fragments arranged by size:
1970 689 92
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1121 2054 2751
Size: 1121 933 697
Fragments arranged by size:
1121 933 697
MscI TGG'CCA
Cuts at: 0 493 1295 2751
Size: 493 802 1456
Fragments arranged by size:
1456 802 493
MslI CAynn'nnrTG
Cuts at: 0 487 2751
Size: 487 2264
MspA1I CmG'CkG
Cuts at: 0 168 186 425 576 594 974 1167 2305
Size: 168 18 239 151 18 380 193 1138
Cuts at: 2305 2751
Size: 446
Fragments arranged by size:
1138 446 380 239 193 168 151 18
18
NarI GG'CG_CC
Cuts at: 0 1971 2063 2751
Size: 1971 92 688
Fragments arranged by size:
1971 688 92
NcoI C'CATG_G
Cuts at: 0 390 2751
Size: 390 2361
NruI TCG'CGA
Cuts at: 0 2548 2751
Size: 2548 203
NspI r_CATG'y
Cuts at: 0 760 2085 2561 2751
Size: 760 1325 476 190
Fragments arranged by size:
1325 760 476 190
PflMI CCAn_nnn'nTGG
Cuts at: 0 126 2751
Size: 126 2625
PfoI T'CCnGG_A
Cuts at: 0 1308 1565 2212 2751
Size: 1308 257 647 539
Fragments arranged by size:
1308 647 539 257
PpuMI rG'GwC_Cy
Cuts at: 0 315 1497 1533 1584 1668 1689 2751
Size: 315 1182 36 51 84 21 1062
Fragments arranged by size:
1182 1062 315 84 51 36 21
PspOMI G'GGCC_C
Cuts at: 0 2014 2751
Size: 2014 737
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 982 1014 2751
Size: 982 32 1737
Fragments arranged by size:
1737 982 32
PstI C_TGCA'G
Cuts at: 0 164 185 1022 2003 2615 2751
Size: 164 21 837 981 612 136
Fragments arranged by size:
981 837 612 164 136 21
PvuII CAG'CTG
Cuts at: 0 168 186 425 594 1167 2751
Size: 168 18 239 169 573 1584
Fragments arranged by size:
1584 573 239 169 168 18
SacI G_AGCT'C
Cuts at: 0 1663 2751
Size: 1663 1088
SacII CC_GC'GG
Cuts at: 0 2306 2751
Size: 2306 445
SalI G'TCGA_C
Cuts at: 0 339 2751
Size: 339 2412
SanDI GG'GwC_CC
Cuts at: 0 1689 2751
Size: 1689 1062
SapI GCTCTTCn'nnn_
Cuts at: 0 952 2751
Size: 952 1799
SexAI A'CCwGG_T
Cuts at: 0 431 2751
Size: 431 2320
SfcI C'TryA_G
Cuts at: 0 160 181 1018 1749 1999 2611 2751
Size: 160 21 837 731 250 612 140
Fragments arranged by size:
837 731 612 250 160 140 21
SfoI GGC'GCC
Cuts at: 0 1972 2064 2751
Size: 1972 92 687
Fragments arranged by size:
1972 687 92
SmaI CCC'GGG
Cuts at: 0 1582 2751
Size: 1582 1169
SmlI C'TyrA_G
Cuts at: 0 768 2101 2359 2751
Size: 768 1333 258 392
Fragments arranged by size:
1333 768 392 258
SphI G_CATG'C
Cuts at: 0 760 2085 2561 2751
Size: 760 1325 476 190
Fragments arranged by size:
1325 760 476 190
StuI AGG'CCT
Cuts at: 0 198 671 2751
Size: 198 473 2080
Fragments arranged by size:
2080 473 198
StyI C'CwwG_G
Cuts at: 0 390 953 1232 1634 2240 2375 2516 2600
Size: 390 563 279 402 606 135 141 84
Cuts at: 2600 2751
Size: 151
Fragments arranged by size:
606 563 402 390 279 151 141 135
84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 403 1287 1341 2751
Size: 403 884 54 1410
Fragments arranged by size:
1410 884 403 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 335 367 2128 2160 2751
Size: 335 32 1761 32 591
Fragments arranged by size:
1761 591 335 32 32
XbaI T'CTAG_A
Cuts at: 0 834 2751
Size: 834 1917
XmaI C'CCGG_G
Cuts at: 0 1580 2751
Size: 1580 1171
XmnI GAAnn'nnTTC
Cuts at: 0 7 2332 2751
Size: 7 2325 419
Fragments arranged by size:
2325 419 7
ZraI GAC'GTC
Cuts at: 0 981 2751
Size: 981 1770
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AleI AlfI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI NcoI NruI NspI PflMI
PfoI PpuMI PspOMI PsrI PstI PvuII SacI SacII
SalI SanDI SapI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TstI XbaI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BclI BfrBI BlpI BmgBI
BmtI BplI BsaAI BsiEI BsiWI BsmI BspHI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PvuI RsrII SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI
Tth111I XcmI XhoI