Restriction map for
pFN21AE5825
(Linear) (Six-base) MAPSORT of: KIEE5825.seq Check: 6490 from: 1 to: 3648
KIEE5825.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 17 3648
Size: 17 3631
AatII G_ACGT'C
Cuts at: 0 146 3648
Size: 146 3502
AccI GT'mk_AC
Cuts at: 0 3234 3648
Size: 3234 414
AclI AA'CG_TT
Cuts at: 0 443 3648
Size: 443 3205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1183 1256 1544 2195 2284 2803 2910
Size: 122 1061 73 288 651 89 519 107
Cuts at: 2910 3648
Size: 738
Fragments arranged by size:
1061 738 651 519 288 122 107 89
73
AleI CACnn'nnGTG
Cuts at: 0 454 1316 1390 1462 1915 3407 3648
Size: 454 862 74 72 453 1492 241
Fragments arranged by size:
1492 862 454 453 241 74 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 674 3648
Size: 674 2974
AlwNI CAG_nnn'CTG
Cuts at: 0 160 919 1567 1756 2110 2314 3648
Size: 160 759 648 189 354 204 1334
Fragments arranged by size:
1334 759 648 354 204 189 160
ApoI r'AATT_y
Cuts at: 0 208 217 818 934 1147 2809 3648
Size: 208 9 601 116 213 1662 839
Fragments arranged by size:
1662 839 601 213 208 116 9
AseI AT'TA_AT
Cuts at: 0 2702 3648
Size: 2702 946
AvaI C'yCGr_G
Cuts at: 0 825 3648
Size: 825 2823
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2564 2597 3648
Size: 2564 33 1051
Fragments arranged by size:
2564 1051 33
BanI G'GyrC_C
Cuts at: 0 5 28 1504 3648
Size: 5 23 1476 2144
Fragments arranged by size:
2144 1476 23 5
BbeI G_GCGC'C
Cuts at: 0 32 3648
Size: 32 3616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3448 3482 3648
Size: 3448 34 166
Fragments arranged by size:
3448 166 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 14 2992 3349 3648
Size: 14 2978 357 299
Fragments arranged by size:
2978 357 299 14
BclI T'GATC_A
Cuts at: 0 3303 3395 3648
Size: 3303 92 253
Fragments arranged by size:
3303 253 92
BfrBI ATG'CAT
Cuts at: 0 3279 3648
Size: 3279 369
BglI GCCn_nnn'nGGC
Cuts at: 0 1897 3648
Size: 1897 1751
BglII A'GATC_T
Cuts at: 0 2871 3648
Size: 2871 777
Bme1580I G_kGCm'C
Cuts at: 0 8 1509 3317 3648
Size: 8 1501 1808 331
Fragments arranged by size:
1808 1501 331 8
BmgBI CAC'GTC
Cuts at: 0 1835 3648
Size: 1835 1813
BmrI ACTGGGnnnn_n'
Cuts at: 0 480 726 1503 1888 2396 3234 3648
Size: 480 246 777 385 508 838 414
Fragments arranged by size:
838 777 508 480 414 385 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2066 2265 2325 3648
Size: 2066 199 60 1323
Fragments arranged by size:
2066 1323 199 60
Bpu10I CC'TnA_GC
Cuts at: 0 2485 3648
Size: 2485 1163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1455 3648
Size: 1455 2193
BsaAI yAC'GTr
Cuts at: 0 1314 1683 3648
Size: 1314 369 1965
Fragments arranged by size:
1965 1314 369
BsaHI Gr'CG_yC
Cuts at: 0 29 143 3648
Size: 29 114 3505
Fragments arranged by size:
3505 114 29
BsaWI w'CCGG_w
Cuts at: 0 488 841 1902 3242 3521 3648
Size: 488 353 1061 1340 279 127
Fragments arranged by size:
1340 1061 488 353 279 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 219 249 3648
Size: 219 30 3399
Fragments arranged by size:
3399 219 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 227 2842 3559 3648
Size: 227 2615 717 89
Fragments arranged by size:
2615 717 227 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1440 2987 3648
Size: 1440 1547 661
Fragments arranged by size:
1547 1440 661
BsiEI CG_ry'CG
Cuts at: 0 1634 3648
Size: 1634 2014
BsmI GAATG_Cn'
Cuts at: 0 81 1997 3648
Size: 81 1916 1651
Fragments arranged by size:
1916 1651 81
BsmBI CGTCTCn'nnnn_
Cuts at: 0 887 2430 3648
Size: 887 1543 1218
Fragments arranged by size:
1543 1218 887
Bsp1286I G_dGCh'C
Cuts at: 0 8 1509 3317 3648
Size: 8 1501 1808 331
Fragments arranged by size:
1808 1501 331 8
BspEI T'CCGG_A
Cuts at: 0 488 1902 3242 3648
Size: 488 1414 1340 406
Fragments arranged by size:
1414 1340 488 406
BspHI T'CATG_A
Cuts at: 0 755 2057 3474 3648
Size: 755 1302 1417 174
Fragments arranged by size:
1417 1302 755 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 17 1420 3648
Size: 17 1403 2228
Fragments arranged by size:
2228 1403 17
BsrDI GCAATG_nn'
Cuts at: 0 2058 3437 3648
Size: 2058 1379 211
Fragments arranged by size:
2058 1379 211
BsrGI T'GTAC_A
Cuts at: 0 3351 3648
Size: 3351 297
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3328 3648
Size: 3328 320
BstBI TT'CG_AA
Cuts at: 0 206 3648
Size: 206 3442
BstEII G'GTnAC_C
Cuts at: 0 312 1046 1821 3648
Size: 312 734 775 1827
Fragments arranged by size:
1827 775 734 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 896 2624 3648
Size: 896 1728 1024
Fragments arranged by size:
1728 1024 896
BstYI r'GATC_y
Cuts at: 0 1213 1563 1842 2871 3648
Size: 1213 350 279 1029 777
Fragments arranged by size:
1213 1029 777 350 279
Bsu36I CC'TnA_GG
Cuts at: 0 1209 1531 3648
Size: 1209 322 2117
Fragments arranged by size:
2117 1209 322
BtgI C'CryG_G
Cuts at: 0 14 953 1634 1826 3648
Size: 14 939 681 192 1822
Fragments arranged by size:
1822 939 681 192 14
BtsI GCAGTG_nn'
Cuts at: 0 870 1064 1340 2198 2357 3037 3290 3402
Size: 870 194 276 858 159 680 253 112
Cuts at: 3402 3414 3648
Size: 12 234
Fragments arranged by size:
870 858 680 276 253 234 194 159
112 12
ClaI AT'CG_AT
Cuts at: 0 2886 3648
Size: 2886 762
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1053 1088 3565 3600 3648
Size: 1053 35 2477 35 48
Fragments arranged by size:
2477 1053 48 35 35
DraI TTT'AAA
Cuts at: 0 2726 3648
Size: 2726 922
DraIII CAC_nnn'GTG
Cuts at: 0 982 3113 3648
Size: 982 2131 535
Fragments arranged by size:
2131 982 535
DrdI GACnn_nn'nnGTC
Cuts at: 0 993 3648
Size: 993 2655
EaeI y'GGCC_r
Cuts at: 0 1631 2477 2789 3151 3648
Size: 1631 846 312 362 497
Fragments arranged by size:
1631 846 497 362 312
EagI C'GGCC_G
Cuts at: 0 1631 3648
Size: 1631 2017
EarI CTCTTCn'nnn_
Cuts at: 0 796 1097 2191 2228 2725 2822 2951 3206
Size: 796 301 1094 37 497 97 129 255
Cuts at: 3206 3648
Size: 442
Fragments arranged by size:
1094 796 497 442 301 255 129 97
37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2137 3648
Size: 2137 1511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1183 1256 1544 2066 2195 2265 2284
Size: 122 1061 73 288 522 129 70 19
Cuts at: 2284 2325 2803 2910 3648
Size: 41 478 107 738
Fragments arranged by size:
1061 738 522 478 288 129 122 107
73 70 41 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 401 1661 2598 3648
Size: 401 1260 937 1050
Fragments arranged by size:
1260 1050 937 401
EcoO109I rG'GnC_Cy
Cuts at: 0 1540 1624 3388 3648
Size: 1540 84 1764 260
Fragments arranged by size:
1764 1540 260 84
EcoRI G'AATT_C
Cuts at: 0 217 3648
Size: 217 3431
EcoRV GAT'ATC
Cuts at: 0 2853 3648
Size: 2853 795
HaeII r_GCGC'y
Cuts at: 0 32 3648
Size: 32 3616
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 892 924 2667 2699 2837 2869 3372 3404
Size: 892 32 1743 32 138 32 503 32
Cuts at: 3404 3648
Size: 244
Fragments arranged by size:
1743 892 503 244 138 32 32 32
32
HincII GTy'rAC
Cuts at: 0 344 998 2269 3486 3648
Size: 344 654 1271 1217 162
Fragments arranged by size:
1271 1217 654 344 162
HindIII A'AGCT_T
Cuts at: 0 134 2611 3648
Size: 134 2477 1037
Fragments arranged by size:
2477 1037 134
HpaI GTT'AAC
Cuts at: 0 344 3648
Size: 344 3304
KasI G'GCGC_C
Cuts at: 0 28 3648
Size: 28 3620
MfeI C'AATT_G
Cuts at: 0 2367 3648
Size: 2367 1281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1930 3387 3648
Size: 1930 1457 261
Fragments arranged by size:
1930 1457 261
MscI TGG'CCA
Cuts at: 0 2479 2791 3153 3648
Size: 2479 312 362 495
Fragments arranged by size:
2479 495 362 312
MslI CAynn'nnrTG
Cuts at: 0 454 894 1316 1390 1462 1725 1915 3190
Size: 454 440 422 74 72 263 190 1275
Cuts at: 3190 3407 3569 3648
Size: 217 162 79
Fragments arranged by size:
1275 454 440 422 263 217 190 162
79 74 72
MspA1I CmG'CkG
Cuts at: 0 579 3648
Size: 579 3069
NarI GG'CG_CC
Cuts at: 0 29 3648
Size: 29 3619
NcoI C'CATG_G
Cuts at: 0 953 1826 3648
Size: 953 873 1822
Fragments arranged by size:
1822 953 873
NdeI CA'TA_TG
Cuts at: 0 1956 2919 3648
Size: 1956 963 729
Fragments arranged by size:
1956 963 729
NsiI A_TGCA'T
Cuts at: 0 3281 3648
Size: 3281 367
PasI CC'CwG_GG
Cuts at: 0 3030 3648
Size: 3030 618
PfoI T'CCnGG_A
Cuts at: 0 1354 2994 3648
Size: 1354 1640 654
Fragments arranged by size:
1640 1354 654
PmlI CAC'GTG
Cuts at: 0 1314 3648
Size: 1314 2334
PpuMI rG'GwC_Cy
Cuts at: 0 1624 3388 3648
Size: 1624 1764 260
Fragments arranged by size:
1764 1624 260
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 451 483 3648
Size: 451 32 3165
Fragments arranged by size:
3165 451 32
PstI C_TGCA'G
Cuts at: 0 915 2549 2871 3333 3347 3409 3648
Size: 915 1634 322 462 14 62 239
Fragments arranged by size:
1634 915 462 322 239 62 14
PvuII CAG'CTG
Cuts at: 0 579 3648
Size: 579 3069
SapI GCTCTTCn'nnn_
Cuts at: 0 3206 3648
Size: 3206 442
ScaI AGT'ACT
Cuts at: 0 1453 3648
Size: 1453 2195
SexAI A'CCwGG_T
Cuts at: 0 1042 1514 3648
Size: 1042 472 2134
Fragments arranged by size:
2134 1042 472
SfcI C'TryA_G
Cuts at: 0 80 911 2545 2867 3170 3231 3329 3343
Size: 80 831 1634 322 303 61 98 14
Cuts at: 3343 3405 3648
Size: 62 243
Fragments arranged by size:
1634 831 322 303 243 98 80 62
61 14
SfoI GGC'GCC
Cuts at: 0 30 3648
Size: 30 3618
SmlI C'TyrA_G
Cuts at: 0 1470 3648
Size: 1470 2178
SspI AAT'ATT
Cuts at: 0 681 1120 1924 3648
Size: 681 439 804 1724
Fragments arranged by size:
1724 804 681 439
StyI C'CwwG_G
Cuts at: 0 953 1826 2707 3648
Size: 953 873 881 941
Fragments arranged by size:
953 941 881 873
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1068 1077 1312 2190 2217 3043 3648
Size: 1068 9 235 878 27 826 605
Fragments arranged by size:
1068 878 826 605 235 27 9
TatI w'GTAC_w
Cuts at: 0 620 973 1451 2116 3351 3551 3648
Size: 620 353 478 665 1235 200 97
Fragments arranged by size:
1235 665 620 478 353 200 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 219 251 296 328 3648
Size: 219 32 45 32 3320
Fragments arranged by size:
3320 219 45 32 32
XbaI T'CTAG_A
Cuts at: 0 1987 3648
Size: 1987 1661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1395 3648
Size: 1395 2253
ZraI GAC'GTC
Cuts at: 0 144 3648
Size: 144 3504
Enzymes that do cut:
AarI AatII AccI AclI AcuI AleI AlfI AlwNI
ApoI AseI AvaI BaeI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI
MslI MspA1I NarI NcoI NdeI NsiI PasI PfoI
PmlI PpuMI PsrI PstI PvuII SapI ScaI SexAI
SfcI SfoI SmlI SspI StyI TaqII TatI TstI
XbaI XcmI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AflIII AgeI AhdI AloI ApaI
ApaLI AscI AsiSI AvrII BamHI BanII BbsI BbvCI
BlpI BmtI BplI BsaI BsaBI BseYI BsiHKAI BsiWI
BsrBI BsrFI BssHII BssSI BstZ17I BtgZI EcoICRI FalI
FseI FspI FspAI KpnI MluI NaeI NgoMIV NheI
NotI NruI NspI PacI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI
XmnI