Restriction map for
pFN21AE5824
(Linear) (Six-base) MAPSORT of: KIEE5824.seq Check: 4376 from: 1 to: 3276
KIEE5824.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1451 1829 1886 2348 2525 2603 3276
Size: 940 511 378 57 462 177 78 673
Fragments arranged by size:
940 673 511 462 378 177 78 57
AflIII A'CryG_T
Cuts at: 0 2148 3276
Size: 2148 1128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1316 3276
Size: 1316 1960
AlwNI CAG_nnn'CTG
Cuts at: 0 52 2238 3276
Size: 52 2186 1038
Fragments arranged by size:
2186 1038 52
ApaLI G'TGCA_C
Cuts at: 0 736 3276
Size: 736 2540
ApoI r'AATT_y
Cuts at: 0 99 360 941 1450 1803 1853 2909 3028
Size: 99 261 581 509 353 50 1056 119
Cuts at: 3028 3276
Size: 248
Fragments arranged by size:
1056 581 509 353 261 248 119 99
50
BanI G'GyrC_C
Cuts at: 0 1157 2766 3276
Size: 1157 1609 510
Fragments arranged by size:
1609 1157 510
BanII G_rGCy'C
Cuts at: 0 247 2583 3276
Size: 247 2336 693
Fragments arranged by size:
2336 693 247
BbsI GAAGACnn'nnnn_
Cuts at: 0 2639 2714 3178 3276
Size: 2639 75 464 98
Fragments arranged by size:
2639 464 98 75
BbvCI CC'TCA_GC
Cuts at: 0 1620 3204 3276
Size: 1620 1584 72
Fragments arranged by size:
1620 1584 72
BciVI GTATCCnnnnn_n'
Cuts at: 0 730 2442 3276
Size: 730 1712 834
Fragments arranged by size:
1712 834 730
BfrBI ATG'CAT
Cuts at: 0 1303 3276
Size: 1303 1973
Bme1580I G_kGCm'C
Cuts at: 0 740 2771 3276
Size: 740 2031 505
Fragments arranged by size:
2031 740 505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2602 3276
Size: 2602 674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 3276
Size: 661 2615
Bpu10I CC'TnA_GC
Cuts at: 0 1620 3204 3276
Size: 1620 1584 72
Fragments arranged by size:
1620 1584 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 2786 3276
Size: 907 1879 490
Fragments arranged by size:
1879 907 490
BsaAI yAC'GTr
Cuts at: 0 2430 3276
Size: 2430 846
BsaBI GATnn'nnATC
Cuts at: 0 618 3257 3276
Size: 618 2639 19
Fragments arranged by size:
2639 618 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2456 3192 3276
Size: 2456 736 84
Fragments arranged by size:
2456 736 84
BseYI C'CCAG_C
Cuts at: 0 962 3276
Size: 962 2314
BsiHKAI G_wGCw'C
Cuts at: 0 740 778 2583 3276
Size: 740 38 1805 693
Fragments arranged by size:
1805 740 693 38
Bsp1286I G_dGCh'C
Cuts at: 0 247 740 778 2583 2771 3276
Size: 247 493 38 1805 188 505
Fragments arranged by size:
1805 505 493 247 188 38
BspHI T'CATG_A
Cuts at: 0 2901 3276
Size: 2901 375
BsrBI CCG'CTC
Cuts at: 0 510 3276
Size: 510 2766
BsrGI T'GTAC_A
Cuts at: 0 616 652 2885 3276
Size: 616 36 2233 391
Fragments arranged by size:
2233 616 391 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3214 3276
Size: 3214 62
BstBI TT'CG_AA
Cuts at: 0 2745 3276
Size: 2745 531
BstEII G'GTnAC_C
Cuts at: 0 1128 3276
Size: 1128 2148
BstYI r'GATC_y
Cuts at: 0 219 558 3276
Size: 219 339 2718
Fragments arranged by size:
2718 339 219
BtsI GCAGTG_nn'
Cuts at: 0 232 1093 3276
Size: 232 861 2183
Fragments arranged by size:
2183 861 232
DraI TTT'AAA
Cuts at: 0 2397 3276
Size: 2397 879
DraIII CAC_nnn'GTG
Cuts at: 0 81 2320 3276
Size: 81 2239 956
Fragments arranged by size:
2239 956 81
EarI CTCTTCn'nnn_
Cuts at: 0 313 740 1054 1114 3276
Size: 313 427 314 60 2162
Fragments arranged by size:
2162 427 314 313 60
EcoICRI GAG'CTC
Cuts at: 0 2581 3276
Size: 2581 695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 940 1451 1829 1886 2348 2525 2603
Size: 661 279 511 378 57 462 177 78
Cuts at: 2603 3276
Size: 673
Fragments arranged by size:
673 661 511 462 378 279 177 78
57
EcoO109I rG'GnC_Cy
Cuts at: 0 2491 3276
Size: 2491 785
EcoRI G'AATT_C
Cuts at: 0 1853 3028 3276
Size: 1853 1175 248
Fragments arranged by size:
1853 1175 248
EcoRV GAT'ATC
Cuts at: 0 65 3276
Size: 65 3211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1339 3276
Size: 1339 1937
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 430 462 1603 1635 2782 2814 3276
Size: 26 404 32 1141 32 1147 32 462
Fragments arranged by size:
1147 1141 462 404 32 32 32 26
HincII GTy'rAC
Cuts at: 0 475 540 1740 3276
Size: 475 65 1200 1536
Fragments arranged by size:
1536 1200 475 65
HindIII A'AGCT_T
Cuts at: 0 1811 1927 3276
Size: 1811 116 1349
Fragments arranged by size:
1811 1349 116
MfeI C'AATT_G
Cuts at: 0 850 1194 2834 3276
Size: 850 344 1640 442
Fragments arranged by size:
1640 850 442 344
MslI CAynn'nnrTG
Cuts at: 0 1252 2525 2531 3251 3276
Size: 1252 1273 6 720 25
Fragments arranged by size:
1273 1252 720 25 6
MspA1I CmG'CkG
Cuts at: 0 496 1624 1693 2001 2515 3276
Size: 496 1128 69 308 514 761
Fragments arranged by size:
1128 761 514 496 308 69
NdeI CA'TA_TG
Cuts at: 0 1115 3276
Size: 1115 2161
NsiI A_TGCA'T
Cuts at: 0 1305 3276
Size: 1305 1971
NspI r_CATG'y
Cuts at: 0 1989 2530 2861 3276
Size: 1989 541 331 415
Fragments arranged by size:
1989 541 415 331
PflMI CCAn_nnn'nTGG
Cuts at: 0 40 81 1947 3276
Size: 40 41 1866 1329
Fragments arranged by size:
1866 1329 41 40
PsiI TTA'TAA
Cuts at: 0 1372 3276
Size: 1372 1904
PstI C_TGCA'G
Cuts at: 0 227 3276
Size: 227 3049
PvuII CAG'CTG
Cuts at: 0 496 1624 1693 2001 2515 3276
Size: 496 1128 69 308 514 761
Fragments arranged by size:
1128 761 514 496 308 69
SacI G_AGCT'C
Cuts at: 0 2583 3276
Size: 2583 693
SapI GCTCTTCn'nnn_
Cuts at: 0 313 1054 3276
Size: 313 741 2222
Fragments arranged by size:
2222 741 313
SfcI C'TryA_G
Cuts at: 0 223 566 1835 2189 3276
Size: 223 343 1269 354 1087
Fragments arranged by size:
1269 1087 354 343 223
SphI G_CATG'C
Cuts at: 0 2861 3276
Size: 2861 415
SspI AAT'ATT
Cuts at: 0 899 1746 3276
Size: 899 847 1530
Fragments arranged by size:
1530 899 847
StyI C'CwwG_G
Cuts at: 0 503 3276
Size: 503 2773
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1175 1597 2537 3276
Size: 1175 422 940 739
Fragments arranged by size:
1175 940 739 422
TatI w'GTAC_w
Cuts at: 0 616 652 1995 2885 3276
Size: 616 36 1343 890 391
Fragments arranged by size:
1343 890 616 391 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 211 243 2853 2885 3276
Size: 211 32 2610 32 391
Fragments arranged by size:
2610 391 211 32 32
XbaI T'CTAG_A
Cuts at: 0 2757 3276
Size: 2757 519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2599 3276
Size: 2599 677
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaLI ApoI BanI BanII
BbsI BbvCI BciVI BfrBI Bme1580I BplI BpmI Bpu10I
BsaI BsaAI BsaBI BseRI BseYI BsiHKAI Bsp1286I BspHI
BsrBI BsrGI BstAPI BstBI BstEII BstYI BtsI DraI
DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I HincII HindIII MfeI MslI MspA1I NdeI NsiI
NspI PflMI PsiI PstI PvuII SacI SapI SfcI
SphI SspI StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BclI BglI BglII
BlpI BmgBI BmrI BmtI BpuEI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrDI
BsrFI BssHII BssSI BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DrdI EaeI EagI EciI EcoNI FseI
FspI FspAI HaeII HpaI KasI KpnI MluI MmeI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI StuI SwaI Tth111I
XhoI XmaI XmnI ZraI