Restriction map for
pFN21AE5818
(Linear) (Six-base) MAPSORT of: KIEE5818.seq Check: 8490 from: 1 to: 2865
KIEE5818.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AclI AA'CG_TT
Cuts at: 0 757 2865
Size: 757 2108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 2086 2137 2865
Size: 245 1841 51 728
Fragments arranged by size:
1841 728 245 51
AflII C'TTAA_G
Cuts at: 0 2557 2865
Size: 2557 308
AleI CACnn'nnGTG
Cuts at: 0 1865 1916 2865
Size: 1865 51 949
Fragments arranged by size:
1865 949 51
AlwNI CAG_nnn'CTG
Cuts at: 0 1657 1756 2865
Size: 1657 99 1109
Fragments arranged by size:
1657 1109 99
ApoI r'AATT_y
Cuts at: 0 1731 2231 2251 2303 2315 2333 2492 2865
Size: 1731 500 20 52 12 18 159 373
Fragments arranged by size:
1731 500 373 159 52 20 18 12
AvaI C'yCGr_G
Cuts at: 0 2544 2865
Size: 2544 321
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1662 1695 2865
Size: 1662 33 1170
Fragments arranged by size:
1662 1170 33
BamHI G'GATC_C
Cuts at: 0 725 2865
Size: 725 2140
BanI G'GyrC_C
Cuts at: 0 1867 2604 2865
Size: 1867 737 261
Fragments arranged by size:
1867 737 261
BbsI GAAGACnn'nnnn_
Cuts at: 0 36 1275 2865
Size: 36 1239 1590
Fragments arranged by size:
1590 1239 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1488 1522 2865
Size: 1488 34 1343
Fragments arranged by size:
1488 1343 34
BfrBI ATG'CAT
Cuts at: 0 2356 2865
Size: 2356 509
BglI GCCn_nnn'nGGC
Cuts at: 0 654 2865
Size: 654 2211
Bme1580I G_kGCm'C
Cuts at: 0 2609 2865
Size: 2609 256
BmrI ACTGGGnnnn_n'
Cuts at: 0 1374 1389 1404 2865
Size: 1374 15 15 1461
Fragments arranged by size:
1461 1374 15 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 2865
Size: 27 2838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1345 2791 2865
Size: 703 642 1446 74
Fragments arranged by size:
1446 703 642 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1896 2865
Size: 1896 969
BsaI GGTCTCn'nnnn_
Cuts at: 0 2265 2865
Size: 2265 600
BsaHI Gr'CG_yC
Cuts at: 0 1124 2865
Size: 1124 1741
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 284 314 439 469 1265 1295 2767 2797
Size: 284 30 125 30 796 30 1472 30
Cuts at: 2797 2865
Size: 68
Fragments arranged by size:
1472 796 284 125 68 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 700 1405 2283 2543 2865
Size: 700 705 878 260 322
Fragments arranged by size:
878 705 700 322 260
BseYI C'CCAG_C
Cuts at: 0 968 1086 2440 2865
Size: 968 118 1354 425
Fragments arranged by size:
1354 968 425 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2444 2865
Size: 2444 421
BsiHKAI G_wGCw'C
Cuts at: 0 1703 2678 2865
Size: 1703 975 187
Fragments arranged by size:
1703 975 187
BsmI GAATG_Cn'
Cuts at: 0 551 1834 2118 2475 2865
Size: 551 1283 284 357 390
Fragments arranged by size:
1283 551 390 357 284
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2809 2865
Size: 2809 56
Bsp1286I G_dGCh'C
Cuts at: 0 1703 2609 2678 2865
Size: 1703 906 69 187
Fragments arranged by size:
1703 906 187 69
BspHI T'CATG_A
Cuts at: 0 593 1217 2865
Size: 593 624 1648
Fragments arranged by size:
1648 624 593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 581 611 2865
Size: 581 30 2254
Fragments arranged by size:
2254 581 30
BsrBI CCG'CTC
Cuts at: 0 1440 2865
Size: 1440 1425
BsrFI r'CCGG_y
Cuts at: 0 1072 2865
Size: 1072 1793
BsrGI T'GTAC_A
Cuts at: 0 371 2865
Size: 371 2494
BssSI C'ACGA_G
Cuts at: 0 2680 2865
Size: 2680 185
BstAPI GCAn_nnn'nTGC
Cuts at: 0 212 1354 2865
Size: 212 1142 1511
Fragments arranged by size:
1511 1142 212
BstBI TT'CG_AA
Cuts at: 0 924 2490 2865
Size: 924 1566 375
Fragments arranged by size:
1566 924 375
BstEII G'GTnAC_C
Cuts at: 0 635 1251 2865
Size: 635 616 1614
Fragments arranged by size:
1614 635 616
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 1918 2865
Size: 279 1639 947
Fragments arranged by size:
1639 947 279
BstYI r'GATC_y
Cuts at: 0 725 1008 2865
Size: 725 283 1857
Fragments arranged by size:
1857 725 283
Bsu36I CC'TnA_GG
Cuts at: 0 2737 2865
Size: 2737 128
BtgI C'CryG_G
Cuts at: 0 502 930 2865
Size: 502 428 1935
Fragments arranged by size:
1935 502 428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1815 2865
Size: 1815 1050
BtsI GCAGTG_nn'
Cuts at: 0 670 701 2541 2620 2865
Size: 670 31 1840 79 245
Fragments arranged by size:
1840 670 245 79 31
ClaI AT'CG_AT
Cuts at: 0 605 2865
Size: 605 2260
DraIII CAC_nnn'GTG
Cuts at: 0 2264 2682 2865
Size: 2264 418 183
Fragments arranged by size:
2264 418 183
EaeI y'GGCC_r
Cuts at: 0 161 2860 2865
Size: 161 2699 5
Fragments arranged by size:
2699 161 5
EarI CTCTTCn'nnn_
Cuts at: 0 18 236 1087 1382 1898 2131 2748 2865
Size: 18 218 851 295 516 233 617 117
Fragments arranged by size:
851 617 516 295 233 218 117 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 703 1345 2086 2137 2791 2865
Size: 245 458 642 741 51 654 74
Fragments arranged by size:
741 654 642 458 245 74 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 892 1875 2423 2865
Size: 892 983 548 442
Fragments arranged by size:
983 892 548 442
EcoRI G'AATT_C
Cuts at: 0 2315 2333 2865
Size: 2315 18 532
Fragments arranged by size:
2315 532 18
EcoRV GAT'ATC
Cuts at: 0 1179 2865
Size: 1179 1686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 566 2865
Size: 566 2299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 941 973 1094 1126 1265 1297
Size: 439 32 470 32 121 32 139 32
Cuts at: 1297 2497 2509 2529 2541 2865
Size: 1200 12 20 12 324
Fragments arranged by size:
1200 470 439 324 139 121 32 32
32 32 20 12 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 2865
Size: 407 2458
MscI TGG'CCA
Cuts at: 0 163 2865
Size: 163 2702
MslI CAynn'nnrTG
Cuts at: 0 367 1075 1865 1916 2865
Size: 367 708 790 51 949
Fragments arranged by size:
949 790 708 367 51
MspA1I CmG'CkG
Cuts at: 0 84 1090 1235 2865
Size: 84 1006 145 1630
Fragments arranged by size:
1630 1006 145 84
NcoI C'CATG_G
Cuts at: 0 502 930 2865
Size: 502 428 1935
Fragments arranged by size:
1935 502 428
NsiI A_TGCA'T
Cuts at: 0 2358 2865
Size: 2358 507
NspI r_CATG'y
Cuts at: 0 499 1080 2638 2865
Size: 499 581 1558 227
Fragments arranged by size:
1558 581 499 227
PflMI CCAn_nnn'nTGG
Cuts at: 0 1005 1456 2865
Size: 1005 451 1409
Fragments arranged by size:
1409 1005 451
PfoI T'CCnGG_A
Cuts at: 0 1322 2865
Size: 1322 1543
PsiI TTA'TAA
Cuts at: 0 1957 2408 2865
Size: 1957 451 457
Fragments arranged by size:
1957 457 451
PstI C_TGCA'G
Cuts at: 0 2569 2627 2865
Size: 2569 58 238
Fragments arranged by size:
2569 238 58
PvuII CAG'CTG
Cuts at: 0 84 1090 1235 2865
Size: 84 1006 145 1630
Fragments arranged by size:
1630 1006 145 84
SapI GCTCTTCn'nnn_
Cuts at: 0 18 1087 2865
Size: 18 1069 1778
Fragments arranged by size:
1778 1069 18
ScaI AGT'ACT
Cuts at: 0 258 2563 2764 2865
Size: 258 2305 201 101
Fragments arranged by size:
2305 258 201 101
SfcI C'TryA_G
Cuts at: 0 1172 2565 2623 2865
Size: 1172 1393 58 242
Fragments arranged by size:
1393 1172 242 58
SmlI C'TyrA_G
Cuts at: 0 1875 2557 2865
Size: 1875 682 308
Fragments arranged by size:
1875 682 308
SpeI A'CTAG_T
Cuts at: 0 253 2865
Size: 253 2612
SphI G_CATG'C
Cuts at: 0 499 1080 2865
Size: 499 581 1785
Fragments arranged by size:
1785 581 499
SspI AAT'ATT
Cuts at: 0 177 1526 2865
Size: 177 1349 1339
Fragments arranged by size:
1349 1339 177
StyI C'CwwG_G
Cuts at: 0 164 502 513 930 1247 2430 2865
Size: 164 338 11 417 317 1183 435
Fragments arranged by size:
1183 435 417 338 317 164 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 65 1877 2865
Size: 65 1812 988
Fragments arranged by size:
1812 988 65
TatI w'GTAC_w
Cuts at: 0 256 371 2561 2762 2865
Size: 256 115 2190 201 103
Fragments arranged by size:
2190 256 201 115 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 284 316 2865
Size: 284 32 2549
Fragments arranged by size:
2549 284 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2437 2865
Size: 2437 428
XmnI GAAnn'nnTTC
Cuts at: 0 1771 1984 2147 2865
Size: 1771 213 163 718
Fragments arranged by size:
1771 718 213 163
Enzymes that do cut:
AclI AcuI AflII AleI AlwNI ApoI AvaI BaeI
BamHI BanI BbsI BcgI BfrBI BglI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI EarI
Eco57MI EcoNI EcoRI EcoRV FalI Hin4I MmeI MscI
MslI MspA1I NcoI NsiI NspI PflMI PfoI PsiI
PstI PvuII SapI ScaI SfcI SmlI SpeI SphI
SspI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BanII BbeI BbvCI BciVI BclI BglII BlpI BmgBI
BmtI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI BspEI
BsrDI BssHII BstZ17I CspCI DraI DrdI EagI EciI
EcoICRI EcoO109I FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI StuI SwaI
Tth111I XbaI XhoI XmaI ZraI