Restriction map for
pFN21AE5817
(Linear) (Six-base) MAPSORT of: KIEE5817.seq Check: 832 from: 1 to: 2541
KIEE5817.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 113 2541
Size: 113 2428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 1398 1651 1859 2236 2474 2541
Size: 103 1295 253 208 377 238 67
Fragments arranged by size:
1295 377 253 238 208 103 67
AfeI AGC'GCT
Cuts at: 0 1159 2541
Size: 1159 1382
AleI CACnn'nnGTG
Cuts at: 0 658 688 1876 2541
Size: 658 30 1188 665
Fragments arranged by size:
1188 665 658 30
AlwNI CAG_nnn'CTG
Cuts at: 0 973 1647 2541
Size: 973 674 894
Fragments arranged by size:
973 894 674
ApaI G_GGCC'C
Cuts at: 0 2061 2541
Size: 2061 480
ApaLI G'TGCA_C
Cuts at: 0 601 2541
Size: 601 1940
ApoI r'AATT_y
Cuts at: 0 265 586 2431 2541
Size: 265 321 1845 110
Fragments arranged by size:
1845 321 265 110
AvaI C'yCGr_G
Cuts at: 0 740 830 2022 2424 2541
Size: 740 90 1192 402 117
Fragments arranged by size:
1192 740 402 117 90
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1702 1735 2541
Size: 1702 33 806
Fragments arranged by size:
1702 806 33
BamHI G'GATC_C
Cuts at: 0 203 2541
Size: 203 2338
BanI G'GyrC_C
Cuts at: 0 101 744 2011 2541
Size: 101 643 1267 530
Fragments arranged by size:
1267 643 530 101
BanII G_rGCy'C
Cuts at: 0 1103 1508 2061 2541
Size: 1103 405 553 480
Fragments arranged by size:
1103 553 480 405
BbsI GAAGACnn'nnnn_
Cuts at: 0 1117 1399 1535 1581 2541
Size: 1117 282 136 46 960
Fragments arranged by size:
1117 960 282 136 46
BciVI GTATCCnnnnn_n'
Cuts at: 0 463 1703 2541
Size: 463 1240 838
Fragments arranged by size:
1240 838 463
BclI T'GATC_A
Cuts at: 0 299 1751 1856 1868 2229 2541
Size: 299 1452 105 12 361 312
Fragments arranged by size:
1452 361 312 299 105 12
BglII A'GATC_T
Cuts at: 0 1983 2493 2541
Size: 1983 510 48
Fragments arranged by size:
1983 510 48
Bme1580I G_kGCm'C
Cuts at: 0 238 605 695 747 2061 2541
Size: 238 367 90 52 1314 480
Fragments arranged by size:
1314 480 367 238 90 52
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 843 2541
Size: 843 1698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1174 1669 2421 2541
Size: 1174 495 752 120
Fragments arranged by size:
1174 752 495 120
Bpu10I CC'TnA_GC
Cuts at: 0 1043 1546 2541
Size: 1043 503 995
Fragments arranged by size:
1043 995 503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 2541
Size: 641 1900
BsaBI GATnn'nnATC
Cuts at: 0 43 2541
Size: 43 2498
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1642 1666 1672 1696 2541
Size: 1642 24 6 24 845
Fragments arranged by size:
1642 845 24 24 6
BseYI C'CCAG_C
Cuts at: 0 2110 2541
Size: 2110 431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 986 1013 2541
Size: 91 895 27 1528
Fragments arranged by size:
1528 895 91 27
BsiHKAI G_wGCw'C
Cuts at: 0 605 1103 1142 1508 2541
Size: 605 498 39 366 1033
Fragments arranged by size:
1033 605 498 366 39
BsmI GAATG_Cn'
Cuts at: 0 476 2541
Size: 476 2065
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1645 2541
Size: 1645 896
Bsp1286I G_dGCh'C
Cuts at: 0 238 605 695 747 1103 1142 1508 2061
Size: 238 367 90 52 356 39 366 553
Cuts at: 2061 2541
Size: 480
Fragments arranged by size:
553 480 367 366 356 238 90 52
39
BspHI T'CATG_A
Cuts at: 0 2304 2541
Size: 2304 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 516 2541
Size: 113 403 2025
Fragments arranged by size:
2025 403 113
BsrDI GCAATG_nn'
Cuts at: 0 405 2541
Size: 405 2136
BsrFI r'CCGG_y
Cuts at: 0 98 2541
Size: 98 2443
BsrGI T'GTAC_A
Cuts at: 0 1760 2372 2541
Size: 1760 612 169
Fragments arranged by size:
1760 612 169
BstAPI GCAn_nnn'nTGC
Cuts at: 0 177 2541
Size: 177 2364
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1472 2390 2541
Size: 1472 918 151
Fragments arranged by size:
1472 918 151
BstYI r'GATC_y
Cuts at: 0 203 509 1983 2493 2541
Size: 203 306 1474 510 48
Fragments arranged by size:
1474 510 306 203 48
BtgI C'CryG_G
Cuts at: 0 48 440 960 1166 2075 2541
Size: 48 392 520 206 909 466
Fragments arranged by size:
909 520 466 392 206 48
BtsI GCAGTG_nn'
Cuts at: 0 2003 2541
Size: 2003 538
DraI TTT'AAA
Cuts at: 0 2316 2541
Size: 2316 225
DraIII CAC_nnn'GTG
Cuts at: 0 177 192 1137 2541
Size: 177 15 945 1404
Fragments arranged by size:
1404 945 177 15
EaeI y'GGCC_r
Cuts at: 0 137 815 1001 1847 2541
Size: 137 678 186 846 694
Fragments arranged by size:
846 694 678 186 137
EarI CTCTTCn'nnn_
Cuts at: 0 82 356 1292 1699 2111 2278 2541
Size: 82 274 936 407 412 167 263
Fragments arranged by size:
936 412 407 274 263 167 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 916 2541
Size: 916 1625
EcoICRI GAG'CTC
Cuts at: 0 1101 1506 2541
Size: 1101 405 1035
Fragments arranged by size:
1101 1035 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 1174 1398 1651 1669 1859 2236 2421
Size: 103 1071 224 253 18 190 377 185
Cuts at: 2421 2474 2541
Size: 53 67
Fragments arranged by size:
1071 377 253 224 190 185 103 67
53 18
EcoO109I rG'GnC_Cy
Cuts at: 0 834 2057 2058 2541
Size: 834 1223 1 483
Fragments arranged by size:
1223 834 483 1
EcoRI G'AATT_C
Cuts at: 0 586 2541
Size: 586 1955
EcoRV GAT'ATC
Cuts at: 0 921 1840 2283 2541
Size: 921 919 443 258
Fragments arranged by size:
921 919 443 258
HaeII r_GCGC'y
Cuts at: 0 201 1161 1473 2541
Size: 201 960 312 1068
Fragments arranged by size:
1068 960 312 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 486 518 639 671 2541
Size: 486 32 121 32 1870
Fragments arranged by size:
1870 486 121 32 32
HincII GTy'rAC
Cuts at: 0 717 2541
Size: 717 1824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 759 1890 2541
Size: 759 1131 651
Fragments arranged by size:
1131 759 651
MscI TGG'CCA
Cuts at: 0 139 1003 1849 2541
Size: 139 864 846 692
Fragments arranged by size:
864 846 692 139
MslI CAynn'nnrTG
Cuts at: 0 608 658 688 1876 2290 2541
Size: 608 50 30 1188 414 251
Fragments arranged by size:
1188 608 414 251 50 30
MspA1I CmG'CkG
Cuts at: 0 50 1011 1554 2370 2541
Size: 50 961 543 816 171
Fragments arranged by size:
961 816 543 171 50
NcoI C'CATG_G
Cuts at: 0 440 2075 2541
Size: 440 1635 466
Fragments arranged by size:
1635 466 440
NdeI CA'TA_TG
Cuts at: 0 678 2541
Size: 678 1863
NruI TCG'CGA
Cuts at: 0 1036 2541
Size: 1036 1505
NspI r_CATG'y
Cuts at: 0 1750 2295 2541
Size: 1750 545 246
Fragments arranged by size:
1750 545 246
PasI CC'CwG_GG
Cuts at: 0 450 2541
Size: 450 2091
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 1094 2541
Size: 192 902 1447
Fragments arranged by size:
1447 902 192
PfoI T'CCnGG_A
Cuts at: 0 989 1142 1595 1646 2541
Size: 989 153 453 51 895
Fragments arranged by size:
989 895 453 153 51
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2110 2142 2541
Size: 2110 32 399
Fragments arranged by size:
2110 399 32
PpuMI rG'GwC_Cy
Cuts at: 0 834 2541
Size: 834 1707
PspOMI G'GGCC_C
Cuts at: 0 2057 2541
Size: 2057 484
PstI C_TGCA'G
Cuts at: 0 2010 2541
Size: 2010 531
PvuII CAG'CTG
Cuts at: 0 1011 1554 2370 2541
Size: 1011 543 816 171
Fragments arranged by size:
1011 816 543 171
SacI G_AGCT'C
Cuts at: 0 1103 1508 2541
Size: 1103 405 1033
Fragments arranged by size:
1103 1033 405
SacII CC_GC'GG
Cuts at: 0 51 2541
Size: 51 2490
SexAI A'CCwGG_T
Cuts at: 0 188 1172 2541
Size: 188 984 1369
Fragments arranged by size:
1369 984 188
SfcI C'TryA_G
Cuts at: 0 1944 2006 2541
Size: 1944 62 535
Fragments arranged by size:
1944 535 62
SmaI CCC'GGG
Cuts at: 0 832 2541
Size: 832 1709
SmlI C'TyrA_G
Cuts at: 0 656 2541
Size: 656 1885
StuI AGG'CCT
Cuts at: 0 2389 2541
Size: 2389 152
StyI C'CwwG_G
Cuts at: 0 440 565 1088 1465 1607 1801 2075 2541
Size: 440 125 523 377 142 194 274 466
Fragments arranged by size:
523 466 440 377 274 194 142 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 821 1120 1567 2459 2541
Size: 298 523 299 447 892 82
Fragments arranged by size:
892 523 447 299 298 82
TatI w'GTAC_w
Cuts at: 0 1658 1718 1760 2372 2541
Size: 1658 60 42 612 169
Fragments arranged by size:
1658 612 169 60 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1022 1054 2541
Size: 1022 32 1487
Fragments arranged by size:
1487 1022 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 447 1357 2094 2541
Size: 447 910 737 447
Fragments arranged by size:
910 737 447 447
XmaI C'CCGG_G
Cuts at: 0 830 2541
Size: 830 1711
XmnI GAAnn'nnTTC
Cuts at: 0 91 1114 1792 2125 2541
Size: 91 1023 678 333 416
Fragments arranged by size:
1023 678 416 333 91
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApaLI ApoI
AvaI BaeI BamHI BanI BanII BbsI BciVI BclI
BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaBI BsaXI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI
DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV HaeII Hin4I HincII MmeI MscI MslI
MspA1I NcoI NdeI NruI NspI PasI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SexAI
SfcI SmaI SmlI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BbeI BbvCI
BcgI BfrBI BglI BlpI BmgBI BmrI BmtI BsaI
BsaAI BsaHI BsaWI BseRI BsiEI BsiWI BspEI BsrBI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DrdI EagI EcoNI FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NgoMIV NheI NotI NsiI PacI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI