Restriction map for
pFN21AE5814
(Linear) (Six-base) MAPSORT of: KIEE5814.seq Check: 46 from: 1 to: 2739
KIEE5814.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1641 1651 2739
Size: 1641 10 1088
Fragments arranged by size:
1641 1088 10
AatII G_ACGT'C
Cuts at: 0 2291 2739
Size: 2291 448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1301 1841 2739
Size: 409 892 540 898
Fragments arranged by size:
898 892 540 409
AflII C'TTAA_G
Cuts at: 0 1573 2739
Size: 1573 1166
AflIII A'CryG_T
Cuts at: 0 1241 2739
Size: 1241 1498
AgeI A'CCGG_T
Cuts at: 0 2545 2739
Size: 2545 194
AhdI GACnn_n'nnGTC
Cuts at: 0 1815 2258 2739
Size: 1815 443 481
Fragments arranged by size:
1815 481 443
AleI CACnn'nnGTG
Cuts at: 0 185 2525 2739
Size: 185 2340 214
Fragments arranged by size:
2340 214 185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 630 2189 2739
Size: 630 1559 550
Fragments arranged by size:
1559 630 550
ApaI G_GGCC'C
Cuts at: 0 2159 2739
Size: 2159 580
ApaLI G'TGCA_C
Cuts at: 0 172 2174 2739
Size: 172 2002 565
Fragments arranged by size:
2002 565 172
ApoI r'AATT_y
Cuts at: 0 779 1558 2007 2111 2467 2739
Size: 779 779 449 104 356 272
Fragments arranged by size:
779 779 449 356 272 104
AseI AT'TA_AT
Cuts at: 0 35 2087 2739
Size: 35 2052 652
Fragments arranged by size:
2052 652 35
AvaI C'yCGr_G
Cuts at: 0 2054 2354 2739
Size: 2054 300 385
Fragments arranged by size:
2054 385 300
BamHI G'GATC_C
Cuts at: 0 1894 2730 2739
Size: 1894 836 9
Fragments arranged by size:
1894 836 9
BanII G_rGCy'C
Cuts at: 0 985 1255 1465 2159 2510 2739
Size: 985 270 210 694 351 229
Fragments arranged by size:
985 694 351 270 229 210
BclI T'GATC_A
Cuts at: 0 1419 1778 2739
Size: 1419 359 961
Fragments arranged by size:
1419 961 359
Bme1580I G_kGCm'C
Cuts at: 0 176 2159 2178 2444 2739
Size: 176 1983 19 266 295
Fragments arranged by size:
1983 295 266 176 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1773 2739
Size: 1773 966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1376 2395 2739
Size: 1376 1019 344
Fragments arranged by size:
1376 1019 344
Bpu10I CC'TnA_GC
Cuts at: 0 1652 2739
Size: 1652 1087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 2739
Size: 1250 1489
BsaI GGTCTCn'nnnn_
Cuts at: 0 2492 2627 2739
Size: 2492 135 112
Fragments arranged by size:
2492 135 112
BsaAI yAC'GTr
Cuts at: 0 16 1244 2100 2739
Size: 16 1228 856 639
Fragments arranged by size:
1228 856 639 16
BsaBI GATnn'nnATC
Cuts at: 0 1071 1534 1732 2739
Size: 1071 463 198 1007
Fragments arranged by size:
1071 1007 463 198
BsaHI Gr'CG_yC
Cuts at: 0 1475 2288 2739
Size: 1475 813 451
Fragments arranged by size:
1475 813 451
BsaWI w'CCGG_w
Cuts at: 0 1869 2545 2739
Size: 1869 676 194
Fragments arranged by size:
1869 676 194
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1595 1800 2442 2739
Size: 1595 205 642 297
Fragments arranged by size:
1595 642 297 205
BseYI C'CCAG_C
Cuts at: 0 1053 2739
Size: 1053 1686
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 664 2319 2674 2739
Size: 646 18 1655 355 65
Fragments arranged by size:
1655 646 355 65 18
BsiHKAI G_wGCw'C
Cuts at: 0 176 911 1085 2178 2739
Size: 176 735 174 1093 561
Fragments arranged by size:
1093 735 561 176 174
BsmI GAATG_Cn'
Cuts at: 0 1646 2739
Size: 1646 1093
Bsp1286I G_dGCh'C
Cuts at: 0 176 911 985 1085 1255 1465 2159 2178
Size: 176 735 74 100 170 210 694 19
Cuts at: 2178 2444 2510 2739
Size: 266 66 229
Fragments arranged by size:
735 694 266 229 210 176 170 100
74 66 19
BspEI T'CCGG_A
Cuts at: 0 1869 2739
Size: 1869 870
BspHI T'CATG_A
Cuts at: 0 2291 2739
Size: 2291 448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 1641 1651 2178 2603 2739
Size: 427 1214 10 527 425 136
Fragments arranged by size:
1214 527 427 425 136 10
BsrBI CCG'CTC
Cuts at: 0 1799 2739
Size: 1799 940
BsrFI r'CCGG_y
Cuts at: 0 2545 2739
Size: 2545 194
BssHII G'CGCG_C
Cuts at: 0 2640 2739
Size: 2640 99
BstEII G'GTnAC_C
Cuts at: 0 1449 2739
Size: 1449 1290
BstYI r'GATC_y
Cuts at: 0 675 1066 1894 2277 2730 2739
Size: 675 391 828 383 453 9
Fragments arranged by size:
828 675 453 391 383 9
Bsu36I CC'TnA_GG
Cuts at: 0 944 2739
Size: 944 1795
BtgI C'CryG_G
Cuts at: 0 233 713 1898 2298 2433 2739
Size: 233 480 1185 400 135 306
Fragments arranged by size:
1185 480 400 306 233 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2127 2325 2739
Size: 2127 198 414
Fragments arranged by size:
2127 414 198
BtsI GCAGTG_nn'
Cuts at: 0 346 443 2520 2532 2739
Size: 346 97 2077 12 207
Fragments arranged by size:
2077 346 207 97 12
DraI TTT'AAA
Cuts at: 0 129 2739
Size: 129 2610
DraIII CAC_nnn'GTG
Cuts at: 0 1449 2400 2439 2739
Size: 1449 951 39 300
Fragments arranged by size:
1449 951 300 39
DrdI GACnn_nn'nnGTC
Cuts at: 0 1356 2739
Size: 1356 1383
EaeI y'GGCC_r
Cuts at: 0 150 1689 1979 2272 2739
Size: 150 1539 290 293 467
Fragments arranged by size:
1539 467 293 290 150
EarI CTCTTCn'nnn_
Cuts at: 0 1185 2739
Size: 1185 1554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2265 2739
Size: 2265 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1301 1376 1841 2395 2739
Size: 409 892 75 465 554 344
Fragments arranged by size:
892 554 465 409 344 75
EcoO109I rG'GnC_Cy
Cuts at: 0 1917 2155 2156 2739
Size: 1917 238 1 583
Fragments arranged by size:
1917 583 238 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2710 2739
Size: 2710 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 214 246 1303 1335 1801 1833
Size: 68 32 114 32 1057 32 466 32
Cuts at: 1833 2244 2276 2437 2469 2739
Size: 411 32 161 32 270
Fragments arranged by size:
1057 466 411 270 161 114 68 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 416 2228 2739
Size: 416 1812 511
Fragments arranged by size:
1812 511 416
HpaI GTT'AAC
Cuts at: 0 416 2739
Size: 416 2323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2480 2739
Size: 2480 259
MscI TGG'CCA
Cuts at: 0 152 1981 2274 2739
Size: 152 1829 293 465
Fragments arranged by size:
1829 465 293 152
MslI CAynn'nnrTG
Cuts at: 0 185 1712 2171 2221 2525 2650 2739
Size: 185 1527 459 50 304 125 89
Fragments arranged by size:
1527 459 304 185 125 89 50
MspA1I CmG'CkG
Cuts at: 0 715 2739
Size: 715 2024
NcoI C'CATG_G
Cuts at: 0 1898 2739
Size: 1898 841
NspI r_CATG'y
Cuts at: 0 864 2739
Size: 864 1875
PflMI CCAn_nnn'nTGG
Cuts at: 0 2727 2739
Size: 2727 12
PspOMI G'GGCC_C
Cuts at: 0 2155 2739
Size: 2155 584
PstI C_TGCA'G
Cuts at: 0 353 1188 2527 2739
Size: 353 835 1339 212
Fragments arranged by size:
1339 835 353 212
SacII CC_GC'GG
Cuts at: 0 716 2739
Size: 716 2023
ScaI AGT'ACT
Cuts at: 0 970 1486 2269 2739
Size: 970 516 783 470
Fragments arranged by size:
970 783 516 470
SfcI C'TryA_G
Cuts at: 0 349 1184 1959 2523 2739
Size: 349 835 775 564 216
Fragments arranged by size:
835 775 564 349 216
SmaI CCC'GGG
Cuts at: 0 2056 2739
Size: 2056 683
SmlI C'TyrA_G
Cuts at: 0 1265 1573 2739
Size: 1265 308 1166
Fragments arranged by size:
1265 1166 308
SspI AAT'ATT
Cuts at: 0 205 1018 2739
Size: 205 813 1721
Fragments arranged by size:
1721 813 205
StyI C'CwwG_G
Cuts at: 0 760 1898 2739
Size: 760 1138 841
Fragments arranged by size:
1138 841 760
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 13 175 753 2392 2739
Size: 13 162 578 1639 347
Fragments arranged by size:
1639 578 347 162 13
TatI w'GTAC_w
Cuts at: 0 968 1484 2138 2267 2669 2739
Size: 968 516 654 129 402 70
Fragments arranged by size:
968 654 516 402 129 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 885 917 1632 1664 2739
Size: 885 32 715 32 1075
Fragments arranged by size:
1075 885 715 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 106 2607 2739
Size: 106 2501 132
Fragments arranged by size:
2501 132 106
XmaI C'CCGG_G
Cuts at: 0 2054 2739
Size: 2054 685
XmnI GAAnn'nnTTC
Cuts at: 0 708 799 2690 2739
Size: 708 91 1891 49
Fragments arranged by size:
1891 708 91 49
ZraI GAC'GTC
Cuts at: 0 2289 2739
Size: 2289 450
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AgeI AhdI AleI
AlfI ApaI ApaLI ApoI AseI AvaI BamHI BanII
BclI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EarI EciI Eco57MI EcoO109I FalI Hin4I HincII
HpaI MmeI MscI MslI MspA1I NcoI NspI PflMI
PspOMI PstI SacII ScaI SfcI SmaI SmlI SspI
StyI TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AloI AlwNI AscI AsiSI
AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BsaXI
BsiEI BsiWI BsmBI BsrDI BsrGI BssSI BstAPI BstBI
BstXI BstZ17I ClaI CspCI EagI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XhoI