Restriction map for
pFN21AE5813
(Linear) (Six-base) MAPSORT of: KIEE5813.seq Check: 8002 from: 1 to: 2778
KIEE5813.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
Acc65I G'GTAC_C
Cuts at: 0 758 2778
Size: 758 2020
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 128 334 590 764 1264 2203 2778
Size: 113 15 206 256 174 500 939 575
Fragments arranged by size:
939 575 500 256 206 174 113 15
AflIII A'CryG_T
Cuts at: 0 336 2778
Size: 336 2442
AleI CACnn'nnGTG
Cuts at: 0 1772 2778
Size: 1772 1006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2725 2778
Size: 2725 53
AlwNI CAG_nnn'CTG
Cuts at: 0 1239 1962 2107 2241 2778
Size: 1239 723 145 134 537
Fragments arranged by size:
1239 723 537 145 134
ApaI G_GGCC'C
Cuts at: 0 825 2778
Size: 825 1953
ApaLI G'TGCA_C
Cuts at: 0 196 514 2778
Size: 196 318 2264
Fragments arranged by size:
2264 318 196
ApoI r'AATT_y
Cuts at: 0 421 645 719 1384 1951 2778
Size: 421 224 74 665 567 827
Fragments arranged by size:
827 665 567 421 224 74
AvaI C'yCGr_G
Cuts at: 0 811 2245 2778
Size: 811 1434 533
Fragments arranged by size:
1434 811 533
AvrII C'CTAG_G
Cuts at: 0 2268 2778
Size: 2268 510
BamHI G'GATC_C
Cuts at: 0 2085 2778
Size: 2085 693
BanI G'GyrC_C
Cuts at: 0 465 758 1128 2176 2778
Size: 465 293 370 1048 602
Fragments arranged by size:
1048 602 465 370 293
BanII G_rGCy'C
Cuts at: 0 479 811 825 1046 1822 2778
Size: 479 332 14 221 776 956
Fragments arranged by size:
956 776 479 332 221 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 578 1108 1625 2778
Size: 578 530 517 1153
Fragments arranged by size:
1153 578 530 517
BbvCI CC'TCA_GC
Cuts at: 0 2046 2778
Size: 2046 732
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1684 1718 2778
Size: 1684 34 1060
Fragments arranged by size:
1684 1060 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1259 2778
Size: 1259 1519
BfrBI ATG'CAT
Cuts at: 0 2702 2778
Size: 2702 76
BglI GCCn_nnn'nGGC
Cuts at: 0 1741 2778
Size: 1741 1037
BlpI GC'TnA_GC
Cuts at: 0 1691 2778
Size: 1691 1087
Bme1580I G_kGCm'C
Cuts at: 0 200 518 825 2778
Size: 200 318 307 1953
Fragments arranged by size:
1953 318 307 200
BmgBI CAC'GTC
Cuts at: 0 981 2778
Size: 981 1797
BmrI ACTGGGnnnn_n'
Cuts at: 0 1809 2778
Size: 1809 969
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 392 2447 2778
Size: 392 2055 331
Fragments arranged by size:
2055 392 331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 859 1132 2129 2135 2211 2778
Size: 216 643 273 997 6 76 567
Fragments arranged by size:
997 643 567 273 216 76 6
Bpu10I CC'TnA_GC
Cuts at: 0 805 1652 2046 2231 2778
Size: 805 847 394 185 547
Fragments arranged by size:
847 805 547 394 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 1015 2547 2778
Size: 1015 1532 231
Fragments arranged by size:
1532 1015 231
BsaAI yAC'GTr
Cuts at: 0 195 2778
Size: 195 2583
BsaHI Gr'CG_yC
Cuts at: 0 965 2778
Size: 965 1813
BsaWI w'CCGG_w
Cuts at: 0 675 761 2778
Size: 675 86 2017
Fragments arranged by size:
2017 675 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 783 813 2635 2665 2778
Size: 783 30 1822 30 113
Fragments arranged by size:
1822 783 113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 523 2005 2778
Size: 19 504 1482 773
Fragments arranged by size:
1482 773 504 19
BseYI C'CCAG_C
Cuts at: 0 438 556 903 993 2033 2038 2399 2657
Size: 438 118 347 90 1040 5 361 258
Cuts at: 2657 2778
Size: 121
Fragments arranged by size:
1040 438 361 347 258 121 118 90
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 642 719 2554 2778
Size: 260 382 77 1835 224
Fragments arranged by size:
1835 382 260 224 77
BsiHKAI G_wGCw'C
Cuts at: 0 200 518 928 1046 1145 2778
Size: 200 318 410 118 99 1633
Fragments arranged by size:
1633 410 318 200 118 99
BsiWI C'GTAC_G
Cuts at: 0 339 2778
Size: 339 2439
BsmBI CGTCTCn'nnnn_
Cuts at: 0 589 2778
Size: 589 2189
Bsp1286I G_dGCh'C
Cuts at: 0 200 479 518 811 825 928 1046 1145
Size: 200 279 39 293 14 103 118 99
Cuts at: 1145 1822 2778
Size: 677 956
Fragments arranged by size:
956 677 293 279 200 118 103 99
39 14
BspHI T'CATG_A
Cuts at: 0 21 2778
Size: 21 2757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 587 2717 2778
Size: 587 2130 61
Fragments arranged by size:
2130 587 61
BsrBI CCG'CTC
Cuts at: 0 855 2778
Size: 855 1923
BsrFI r'CCGG_y
Cuts at: 0 1004 2614 2630 2778
Size: 1004 1610 16 148
Fragments arranged by size:
1610 1004 148 16
BsrGI T'GTAC_A
Cuts at: 0 947 2651 2778
Size: 947 1704 127
Fragments arranged by size:
1704 947 127
BstAPI GCAn_nnn'nTGC
Cuts at: 0 285 1239 2241 2778
Size: 285 954 1002 537
Fragments arranged by size:
1002 954 537 285
BstBI TT'CG_AA
Cuts at: 0 1616 2778
Size: 1616 1162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1321 1680 2259 2361 2778
Size: 1321 359 579 102 417
Fragments arranged by size:
1321 579 417 359 102
BstYI r'GATC_y
Cuts at: 0 2085 2778
Size: 2085 693
Bsu36I CC'TnA_GG
Cuts at: 0 2371 2778
Size: 2371 407
BtgI C'CryG_G
Cuts at: 0 1133 1314 1639 1773 2300 2354 2778
Size: 1133 181 325 134 527 54 424
Fragments arranged by size:
1133 527 424 325 181 134 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2327 2778
Size: 2327 451
BtsI GCAGTG_nn'
Cuts at: 0 1237 1632 1767 2469 2671 2778
Size: 1237 395 135 702 202 107
Fragments arranged by size:
1237 702 395 202 135 107
DraIII CAC_nnn'GTG
Cuts at: 0 1462 2778
Size: 1462 1316
EaeI y'GGCC_r
Cuts at: 0 545 1323 2549 2606 2694 2778
Size: 545 778 1226 57 88 84
Fragments arranged by size:
1226 778 545 88 84 57
EarI CTCTTCn'nnn_
Cuts at: 0 147 500 1408 2438 2778
Size: 147 353 908 1030 340
Fragments arranged by size:
1030 908 353 340 147
EcoICRI GAG'CTC
Cuts at: 0 1044 2778
Size: 1044 1734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 128 216 334 590 764 859 1132
Size: 113 15 88 118 256 174 95 273
Cuts at: 1132 1264 2129 2135 2203 2211 2778
Size: 132 865 6 68 8 567
Fragments arranged by size:
865 567 273 256 174 132 118 113
95 88 68 15 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2682 2770 2778
Size: 2682 88 8
Fragments arranged by size:
2682 88 8
EcoO109I rG'GnC_Cy
Cuts at: 0 821 2514 2724 2778
Size: 821 1693 210 54
Fragments arranged by size:
1693 821 210 54
EcoRI G'AATT_C
Cuts at: 0 1951 2778
Size: 1951 827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2172 2778
Size: 2172 606
HaeII r_GCGC'y
Cuts at: 0 2649 2778
Size: 2649 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 670 702 935 967 1328 1360
Size: 358 32 280 32 233 32 361 32
Cuts at: 1360 1480 1512 2778
Size: 120 32 1266
Fragments arranged by size:
1266 361 358 280 233 120 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1511 2778
Size: 1511 1267
KpnI G_GTAC'C
Cuts at: 0 762 2778
Size: 762 2016
MfeI C'AATT_G
Cuts at: 0 1935 2778
Size: 1935 843
MluI A'CGCG_T
Cuts at: 0 336 2778
Size: 336 2442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 1185 1289 1359 1697 2778
Size: 713 472 104 70 338 1081
Fragments arranged by size:
1081 713 472 338 104 70
MscI TGG'CCA
Cuts at: 0 547 1325 2551 2608 2778
Size: 547 778 1226 57 170
Fragments arranged by size:
1226 778 547 170 57
MslI CAynn'nnrTG
Cuts at: 0 399 1138 1195 1319 1606 1714 1772 1891
Size: 399 739 57 124 287 108 58 119
Cuts at: 1891 2359 2463 2491 2778
Size: 468 104 28 287
Fragments arranged by size:
739 468 399 287 287 124 119 108
104 58 57 28
MspA1I CmG'CkG
Cuts at: 0 282 2050 2657 2778
Size: 282 1768 607 121
Fragments arranged by size:
1768 607 282 121
NaeI GCC'GGC
Cuts at: 0 1006 2778
Size: 1006 1772
NcoI C'CATG_G
Cuts at: 0 1133 1639 2778
Size: 1133 506 1139
Fragments arranged by size:
1139 1133 506
NdeI CA'TA_TG
Cuts at: 0 1293 2778
Size: 1293 1485
NgoMIV G'CCGG_C
Cuts at: 0 1004 2778
Size: 1004 1774
NsiI A_TGCA'T
Cuts at: 0 2704 2778
Size: 2704 74
NspI r_CATG'y
Cuts at: 0 1537 1605 2328 2778
Size: 1537 68 723 450
Fragments arranged by size:
1537 723 450 68
PasI CC'CwG_GG
Cuts at: 0 870 2778
Size: 870 1908
PflMI CCAn_nnn'nTGG
Cuts at: 0 1416 2778
Size: 1416 1362
PfoI T'CCnGG_A
Cuts at: 0 1791 2088 2778
Size: 1791 297 690
Fragments arranged by size:
1791 690 297
PmlI CAC'GTG
Cuts at: 0 195 2778
Size: 195 2583
PpuMI rG'GwC_Cy
Cuts at: 0 2514 2778
Size: 2514 264
PspOMI G'GGCC_C
Cuts at: 0 821 2778
Size: 821 1957
PstI C_TGCA'G
Cuts at: 0 299 351 1235 1627 2006 2778
Size: 299 52 884 392 379 772
Fragments arranged by size:
884 772 392 379 299 52
PvuII CAG'CTG
Cuts at: 0 282 2050 2657 2778
Size: 282 1768 607 121
Fragments arranged by size:
1768 607 282 121
SacI G_AGCT'C
Cuts at: 0 1046 2778
Size: 1046 1732
SexAI A'CCwGG_T
Cuts at: 0 539 1661 2778
Size: 539 1122 1117
Fragments arranged by size:
1122 1117 539
SfcI C'TryA_G
Cuts at: 0 295 347 1231 1623 2002 2778
Size: 295 52 884 392 379 776
Fragments arranged by size:
884 776 392 379 295 52
SmaI CCC'GGG
Cuts at: 0 813 2778
Size: 813 1965
StuI AGG'CCT
Cuts at: 0 452 2778
Size: 452 2326
StyI C'CwwG_G
Cuts at: 0 1117 1133 1639 1985 2012 2207 2268 2534
Size: 1117 16 506 346 27 195 61 266
Cuts at: 2534 2778
Size: 244
Fragments arranged by size:
1117 506 346 266 244 195 61 27
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 1146 2400 2649 2653 2778
Size: 763 383 1254 249 4 125
Fragments arranged by size:
1254 763 383 249 125 4
TatI w'GTAC_w
Cuts at: 0 947 1280 1697 2651 2778
Size: 947 333 417 954 127
Fragments arranged by size:
954 947 417 333 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 783 815 1420 1452 2379 2411 2778
Size: 783 32 605 32 927 32 367
Fragments arranged by size:
927 783 605 367 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1930 2778
Size: 1930 848
XbaI T'CTAG_A
Cuts at: 0 303 2778
Size: 303 2475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2589 2778
Size: 2589 189
XmaI C'CCGG_G
Cuts at: 0 811 2778
Size: 811 1967
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacI SexAI SfcI
SmaI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI BaeI BbeI BclI BglII
BmtI BpuEI BsaBI BsiEI BsmI BspEI BsrDI BssHII
BssSI BstEII BstZ17I ClaI CspCI DraI DrdI EagI
EciI EcoRV FseI FspI FspAI HincII HpaI KasI
NarI NheI NotI NruI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI XhoI
XmnI ZraI