Restriction map for
pFN21AE5811
(Linear) (Six-base) MAPSORT of: KIEE5811.seq Check: 937 from: 1 to: 2670
KIEE5811.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1159 2670
Size: 1159 1511
AatII G_ACGT'C
Cuts at: 0 1644 2670
Size: 1644 1026
AccI GT'mk_AC
Cuts at: 0 1954 2670
Size: 1954 716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 928 2464 2670
Size: 755 173 1536 206
Fragments arranged by size:
1536 755 206 173
AflIII A'CryG_T
Cuts at: 0 1250 2670
Size: 1250 1420
AgeI A'CCGG_T
Cuts at: 0 1995 2670
Size: 1995 675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 931 2670
Size: 931 1739
AlwNI CAG_nnn'CTG
Cuts at: 0 1197 2593 2670
Size: 1197 1396 77
Fragments arranged by size:
1396 1197 77
ApaI G_GGCC'C
Cuts at: 0 260 2670
Size: 260 2410
ApaLI G'TGCA_C
Cuts at: 0 2299 2670
Size: 2299 371
ApoI r'AATT_y
Cuts at: 0 322 576 782 890 1791 1941 2664 2670
Size: 322 254 206 108 901 150 723 6
Fragments arranged by size:
901 723 322 254 206 150 108 6
AvaI C'yCGr_G
Cuts at: 0 310 2339 2670
Size: 310 2029 331
Fragments arranged by size:
2029 331 310
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2203 2236 2670
Size: 2203 33 434
Fragments arranged by size:
2203 434 33
BamHI G'GATC_C
Cuts at: 0 1437 2670
Size: 1437 1233
BanI G'GyrC_C
Cuts at: 0 118 2670
Size: 118 2552
BanII G_rGCy'C
Cuts at: 0 260 1089 2131 2670
Size: 260 829 1042 539
Fragments arranged by size:
1042 829 539 260
BbsI GAAGACnn'nnnn_
Cuts at: 0 51 251 608 934 2670
Size: 51 200 357 326 1736
Fragments arranged by size:
1736 357 326 200 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1122 1156 2670
Size: 1122 34 1514
Fragments arranged by size:
1514 1122 34
BclI T'GATC_A
Cuts at: 0 569 635 2670
Size: 569 66 2035
Fragments arranged by size:
2035 569 66
BglI GCCn_nnn'nGGC
Cuts at: 0 264 2670
Size: 264 2406
BlpI GC'TnA_GC
Cuts at: 0 1129 1462 2670
Size: 1129 333 1208
Fragments arranged by size:
1208 1129 333
Bme1580I G_kGCm'C
Cuts at: 0 121 260 759 2303 2670
Size: 121 139 499 1544 367
Fragments arranged by size:
1544 499 367 139 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 441 596 685 939 1090 2036 2670
Size: 441 155 89 254 151 946 634
Fragments arranged by size:
946 634 441 254 155 151 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1514 1738 2555 2670
Size: 1514 224 817 115
Fragments arranged by size:
1514 817 224 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1849 2670
Size: 1849 821
BsaHI Gr'CG_yC
Cuts at: 0 73 248 1641 2670
Size: 73 175 1393 1029
Fragments arranged by size:
1393 1029 175 73
BsaWI w'CCGG_w
Cuts at: 0 1995 2670
Size: 1995 675
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 310 340 1974 2004 2020 2050
Size: 148 30 132 30 1634 30 16 30
Cuts at: 2050 2143 2173 2670
Size: 93 30 497
Fragments arranged by size:
1634 497 148 132 93 30 30 30
30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 1390 1752 2576 2670
Size: 238 1152 362 824 94
Fragments arranged by size:
1152 824 362 238 94
BseYI C'CCAG_C
Cuts at: 0 986 2047 2063 2174 2222 2670
Size: 986 1061 16 111 48 448
Fragments arranged by size:
1061 986 448 111 48 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 1918 2017 2670
Size: 899 1019 99 653
Fragments arranged by size:
1019 899 653 99
BsiEI CG_ry'CG
Cuts at: 0 98 104 2670
Size: 98 6 2566
Fragments arranged by size:
2566 98 6
BsiHKAI G_wGCw'C
Cuts at: 0 158 2303 2311 2500 2670
Size: 158 2145 8 189 170
Fragments arranged by size:
2145 189 170 158 8
Bsp1286I G_dGCh'C
Cuts at: 0 121 158 260 759 1089 2131 2303 2311
Size: 121 37 102 499 330 1042 172 8
Cuts at: 2311 2500 2670
Size: 189 170
Fragments arranged by size:
1042 499 330 189 172 170 121 102
37 8
BspHI T'CATG_A
Cuts at: 0 572 2670
Size: 572 2098
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1159 2039 2199 2670
Size: 1159 880 160 471
Fragments arranged by size:
1159 880 471 160
BsrBI CCG'CTC
Cuts at: 0 2140 2670
Size: 2140 530
BsrFI r'CCGG_y
Cuts at: 0 93 142 228 1995 2670
Size: 93 49 86 1767 675
Fragments arranged by size:
1767 675 93 86 49
BsrGI T'GTAC_A
Cuts at: 0 2000 2121 2670
Size: 2000 121 549
Fragments arranged by size:
2000 549 121
BstBI TT'CG_AA
Cuts at: 0 242 2670
Size: 242 2428
BstXI CCAn_nnnn'nTGG
Cuts at: 0 693 882 934 986 2670
Size: 693 189 52 52 1684
Fragments arranged by size:
1684 693 189 52 52
BstYI r'GATC_y
Cuts at: 0 1437 1821 2670
Size: 1437 384 849
Fragments arranged by size:
1437 849 384
BtgI C'CryG_G
Cuts at: 0 98 520 1520 1694 2102 2670
Size: 98 422 1000 174 408 568
Fragments arranged by size:
1000 568 422 408 174 98
BtsI GCAGTG_nn'
Cuts at: 0 887 2670
Size: 887 1783
DraI TTT'AAA
Cuts at: 0 653 998 2670
Size: 653 345 1672
Fragments arranged by size:
1672 653 345
DrdI GACnn_nn'nnGTC
Cuts at: 0 2016 2670
Size: 2016 654
EaeI y'GGCC_r
Cuts at: 0 95 101 1170 2135 2670
Size: 95 6 1069 965 535
Fragments arranged by size:
1069 965 535 95 6
EagI C'GGCC_G
Cuts at: 0 95 101 2670
Size: 95 6 2569
Fragments arranged by size:
2569 95 6
EarI CTCTTCn'nnn_
Cuts at: 0 30 839 1051 2527 2603 2670
Size: 30 809 212 1476 76 67
Fragments arranged by size:
1476 809 212 76 67 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2349 2670
Size: 2349 321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 928 1514 1738 2464 2555 2670
Size: 755 173 586 224 726 91 115
Fragments arranged by size:
755 726 586 224 173 115 91
EcoO109I rG'GnC_Cy
Cuts at: 0 256 257 1713 2670
Size: 256 1 1456 957
Fragments arranged by size:
1456 957 256 1
EcoRI G'AATT_C
Cuts at: 0 1941 2670
Size: 1941 729
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2627 2670
Size: 2627 43
FseI GG_CCGG'CC
Cuts at: 0 97 2670
Size: 97 2573
HaeII r_GCGC'y
Cuts at: 0 114 2320 2670
Size: 114 2206 350
Fragments arranged by size:
2206 350 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 852 884 1487 1519 1974 2006
Size: 664 32 156 32 603 32 455 32
Cuts at: 2006 2670
Size: 664
Fragments arranged by size:
664 664 603 455 156 32 32 32
32
HincII GTy'rAC
Cuts at: 0 318 672 1386 2670
Size: 318 354 714 1284
Fragments arranged by size:
1284 714 354 318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 465 1378 2550 2670
Size: 441 24 913 1172 120
Fragments arranged by size:
1172 913 441 120 24
MslI CAynn'nnrTG
Cuts at: 0 298 691 907 932 2670
Size: 298 393 216 25 1738
Fragments arranged by size:
1738 393 298 216 25
MspA1I CmG'CkG
Cuts at: 0 100 1273 2104 2178 2593 2670
Size: 100 1173 831 74 415 77
Fragments arranged by size:
1173 831 415 100 77 74
NaeI GCC'GGC
Cuts at: 0 95 144 230 2670
Size: 95 49 86 2440
Fragments arranged by size:
2440 95 86 49
NcoI C'CATG_G
Cuts at: 0 520 2670
Size: 520 2150
NdeI CA'TA_TG
Cuts at: 0 864 2670
Size: 864 1806
NgoMIV G'CCGG_C
Cuts at: 0 93 142 228 2670
Size: 93 49 86 2442
Fragments arranged by size:
2442 93 86 49
NspI r_CATG'y
Cuts at: 0 339 1068 1107 1254 2670
Size: 339 729 39 147 1416
Fragments arranged by size:
1416 729 339 147 39
PciI A'CATG_T
Cuts at: 0 1250 2670
Size: 1250 1420
PflMI CCAn_nnn'nTGG
Cuts at: 0 1197 2670
Size: 1197 1473
PfoI T'CCnGG_A
Cuts at: 0 620 1715 2670
Size: 620 1095 955
Fragments arranged by size:
1095 955 620
PpuMI rG'GwC_Cy
Cuts at: 0 1713 2670
Size: 1713 957
PshAI GACnn'nnGTC
Cuts at: 0 2461 2532 2670
Size: 2461 71 138
Fragments arranged by size:
2461 138 71
PsiI TTA'TAA
Cuts at: 0 451 2670
Size: 451 2219
PspOMI G'GGCC_C
Cuts at: 0 256 2670
Size: 256 2414
PstI C_TGCA'G
Cuts at: 0 308 390 968 2113 2670
Size: 308 82 578 1145 557
Fragments arranged by size:
1145 578 557 308 82
PvuII CAG'CTG
Cuts at: 0 1273 2178 2593 2670
Size: 1273 905 415 77
Fragments arranged by size:
1273 905 415 77
SacII CC_GC'GG
Cuts at: 0 101 2105 2670
Size: 101 2004 565
Fragments arranged by size:
2004 565 101
SapI GCTCTTCn'nnn_
Cuts at: 0 2603 2670
Size: 2603 67
SfcI C'TryA_G
Cuts at: 0 304 386 964 2109 2670
Size: 304 82 578 1145 561
Fragments arranged by size:
1145 578 561 304 82
SgrAI Cr'CCGG_yG
Cuts at: 0 1995 2670
Size: 1995 675
SmaI CCC'GGG
Cuts at: 0 312 2341 2670
Size: 312 2029 329
Fragments arranged by size:
2029 329 312
SmlI C'TyrA_G
Cuts at: 0 1864 2670
Size: 1864 806
SspI AAT'ATT
Cuts at: 0 1344 2670
Size: 1344 1326
StyI C'CwwG_G
Cuts at: 0 68 520 1317 2670
Size: 68 452 797 1353
Fragments arranged by size:
1353 797 452 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 978 1781 2670
Size: 978 803 889
Fragments arranged by size:
978 889 803
TatI w'GTAC_w
Cuts at: 0 2000 2121 2670
Size: 2000 121 549
Fragments arranged by size:
2000 549 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 2143 2175 2670
Size: 148 32 1963 32 495
Fragments arranged by size:
1963 495 148 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 983 2670
Size: 983 1687
XmaI C'CCGG_G
Cuts at: 0 310 2339 2670
Size: 310 2029 331
Fragments arranged by size:
2029 331 310
XmnI GAAnn'nnTTC
Cuts at: 0 729 1343 2670
Size: 729 614 1327
Fragments arranged by size:
1327 729 614
ZraI GAC'GTC
Cuts at: 0 1642 2670
Size: 1642 1028
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbsI BcgI BclI BglI BlpI Bme1580I BmrI BpmI
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstBI
BstXI BstYI BtgI BtsI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FseI HaeII
Hin4I HincII MmeI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PpuMI PshAI PsiI
PspOMI PstI PvuII SacII SapI SfcI SgrAI SmaI
SmlI SspI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AhdI AleI AloI AscI
AseI AsiSI AvrII BbeI BbvCI BciVI BfrBI BglII
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsiWI
BsmI BsmBI BspEI BsrDI BssHII BssSI BstAPI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EcoICRI EcoNI
EcoRV FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MscI NarI NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XbaI XhoI