Restriction map for
pFN21AE5809
(Linear) (Six-base) MAPSORT of: KIEE5809.seq Check: 1797 from: 1 to: 2739
KIEE5809.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1255 2739
Size: 452 803 1484
Fragments arranged by size:
1484 803 452
AflIII A'CryG_T
Cuts at: 0 1102 1360 2520 2739
Size: 1102 258 1160 219
Fragments arranged by size:
1160 1102 258 219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2501 2739
Size: 2501 238
AlwNI CAG_nnn'CTG
Cuts at: 0 93 2739
Size: 93 2646
ApoI r'AATT_y
Cuts at: 0 1172 1285 1587 1631 1698 2539 2680 2739
Size: 1172 113 302 44 67 841 141 59
Fragments arranged by size:
1172 841 302 141 113 67 59 44
AseI AT'TA_AT
Cuts at: 0 2669 2739
Size: 2669 70
BanII G_rGCy'C
Cuts at: 0 593 834 2642 2739
Size: 593 241 1808 97
Fragments arranged by size:
1808 593 241 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 2739
Size: 359 2380
BciVI GTATCCnnnnn_n'
Cuts at: 0 551 2739
Size: 551 2188
BclI T'GATC_A
Cuts at: 0 1049 1068 2739
Size: 1049 19 1671
Fragments arranged by size:
1671 1049 19
BfrBI ATG'CAT
Cuts at: 0 1495 2739
Size: 1495 1244
BlpI GC'TnA_GC
Cuts at: 0 963 2739
Size: 963 1776
Bme1580I G_kGCm'C
Cuts at: 0 1060 2739
Size: 1060 1679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1829 2739
Size: 1829 910
Bpu10I CC'TnA_GC
Cuts at: 0 94 482 2739
Size: 94 388 2257
Fragments arranged by size:
2257 388 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 648 2739
Size: 358 290 2091
Fragments arranged by size:
2091 358 290
BsaI GGTCTCn'nnnn_
Cuts at: 0 100 1272 2739
Size: 100 1172 1467
Fragments arranged by size:
1467 1172 100
BsaAI yAC'GTr
Cuts at: 0 1103 1722 2739
Size: 1103 619 1017
Fragments arranged by size:
1103 1017 619
BsaHI Gr'CG_yC
Cuts at: 0 1920 2739
Size: 1920 819
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1379 1409 2739
Size: 1379 30 1330
Fragments arranged by size:
1379 1330 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1517 2069 2739
Size: 1517 552 670
Fragments arranged by size:
1517 670 552
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 2363 2739
Size: 548 1815 376
Fragments arranged by size:
1815 548 376
BsiHKAI G_wGCw'C
Cuts at: 0 593 2642 2739
Size: 593 2049 97
Fragments arranged by size:
2049 593 97
BsmI GAATG_Cn'
Cuts at: 0 1323 2739
Size: 1323 1416
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1911 2739
Size: 1911 828
Bsp1286I G_dGCh'C
Cuts at: 0 593 834 1060 2642 2739
Size: 593 241 226 1582 97
Fragments arranged by size:
1582 593 241 226 97
BspHI T'CATG_A
Cuts at: 0 1974 2696 2739
Size: 1974 722 43
Fragments arranged by size:
1974 722 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1497 2402 2739
Size: 1497 905 337
Fragments arranged by size:
1497 905 337
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2233 2739
Size: 2233 506
BstEII G'GTnAC_C
Cuts at: 0 1179 2739
Size: 1179 1560
BstYI r'GATC_y
Cuts at: 0 12 395 2739
Size: 12 383 2344
Fragments arranged by size:
2344 383 12
Bsu36I CC'TnA_GG
Cuts at: 0 163 2739
Size: 163 2576
BtgI C'CryG_G
Cuts at: 0 1369 2739
Size: 1369 1370
BtsI GCAGTG_nn'
Cuts at: 0 1328 2375 2739
Size: 1328 1047 364
Fragments arranged by size:
1328 1047 364
DraI TTT'AAA
Cuts at: 0 1734 2739
Size: 1734 1005
DraIII CAC_nnn'GTG
Cuts at: 0 532 1066 2739
Size: 532 534 1673
Fragments arranged by size:
1673 534 532
EaeI y'GGCC_r
Cuts at: 0 762 2739
Size: 762 1977
EarI CTCTTCn'nnn_
Cuts at: 0 598 1247 1790 2087 2739
Size: 598 649 543 297 652
Fragments arranged by size:
652 649 598 543 297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 307 2739
Size: 46 261 2432
Fragments arranged by size:
2432 261 46
EcoICRI GAG'CTC
Cuts at: 0 591 2640 2739
Size: 591 2049 99
Fragments arranged by size:
2049 591 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1255 1829 2739
Size: 452 803 574 910
Fragments arranged by size:
910 803 574 452
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2318 2739
Size: 2318 421
EcoO109I rG'GnC_Cy
Cuts at: 0 1236 2739
Size: 1236 1503
EcoRV GAT'ATC
Cuts at: 0 1607 2431 2739
Size: 1607 824 308
Fragments arranged by size:
1607 824 308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 689 2215 2517 2739
Size: 689 1526 302 222
Fragments arranged by size:
1526 689 302 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1026 1058 1126 1158 2387 2419 2739
Size: 1026 32 68 32 1229 32 320
Fragments arranged by size:
1229 1026 320 68 32 32 32
HincII GTy'rAC
Cuts at: 0 1145 2739
Size: 1145 1594
HindIII A'AGCT_T
Cuts at: 0 1515 2739
Size: 1515 1224
MfeI C'AATT_G
Cuts at: 0 1837 2739
Size: 1837 902
MluI A'CGCG_T
Cuts at: 0 1360 2739
Size: 1360 1379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 2739
Size: 323 2416
MscI TGG'CCA
Cuts at: 0 764 2739
Size: 764 1975
MslI CAynn'nnrTG
Cuts at: 0 934 1426 1624 1849 2512 2739
Size: 934 492 198 225 663 227
Fragments arranged by size:
934 663 492 227 225 198
MspA1I CmG'CkG
Cuts at: 0 952 1275 2721 2739
Size: 952 323 1446 18
Fragments arranged by size:
1446 952 323 18
NcoI C'CATG_G
Cuts at: 0 1369 2739
Size: 1369 1370
NsiI A_TGCA'T
Cuts at: 0 1497 2739
Size: 1497 1242
NspI r_CATG'y
Cuts at: 0 1350 2482 2524 2695 2739
Size: 1350 1132 42 171 44
Fragments arranged by size:
1350 1132 171 44 42
PciI A'CATG_T
Cuts at: 0 2520 2739
Size: 2520 219
PfoI T'CCnGG_A
Cuts at: 0 17 1755 1806 2739
Size: 17 1738 51 933
Fragments arranged by size:
1738 933 51 17
PpuMI rG'GwC_Cy
Cuts at: 0 1236 2739
Size: 1236 1503
PsiI TTA'TAA
Cuts at: 0 1849 2739
Size: 1849 890
PvuII CAG'CTG
Cuts at: 0 952 1275 2721 2739
Size: 952 323 1446 18
Fragments arranged by size:
1446 952 323 18
SacI G_AGCT'C
Cuts at: 0 593 2642 2739
Size: 593 2049 97
Fragments arranged by size:
2049 593 97
SapI GCTCTTCn'nnn_
Cuts at: 0 598 2739
Size: 598 2141
ScaI AGT'ACT
Cuts at: 0 2017 2739
Size: 2017 722
SexAI A'CCwGG_T
Cuts at: 0 1238 2739
Size: 1238 1501
SfcI C'TryA_G
Cuts at: 0 953 2156 2739
Size: 953 1203 583
Fragments arranged by size:
1203 953 583
SmlI C'TyrA_G
Cuts at: 0 337 663 2739
Size: 337 326 2076
Fragments arranged by size:
2076 337 326
SnaBI TAC'GTA
Cuts at: 0 1722 2739
Size: 1722 1017
SspI AAT'ATT
Cuts at: 0 556 769 1163 2103 2455 2739
Size: 556 213 394 940 352 284
Fragments arranged by size:
940 556 394 352 284 213
StyI C'CwwG_G
Cuts at: 0 406 1369 1898 1998 2739
Size: 406 963 529 100 741
Fragments arranged by size:
963 741 529 406 100
TatI w'GTAC_w
Cuts at: 0 650 2015 2739
Size: 650 1365 724
Fragments arranged by size:
1365 724 650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1350 1379 1382 1411 2739
Size: 1350 29 3 29 1328
Fragments arranged by size:
1350 1328 29 29 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1896 2739
Size: 1896 843
XmnI GAAnn'nnTTC
Cuts at: 0 1678 2670 2739
Size: 1678 992 69
Fragments arranged by size:
1678 992 69
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI AseI BanII BbsI
BciVI BclI BfrBI BlpI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BstAPI BstEII BstYI Bsu36I
BtgI BtsI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII HindIII
MfeI MluI MmeI MscI MslI MspA1I NcoI NsiI
NspI PciI PfoI PpuMI PsiI PvuII SacI SapI
ScaI SexAI SfcI SmlI SnaBI SspI StyI TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BglI
BglII BmgBI BmrI BmtI BplI BsaBI BsaWI BseYI
BsiEI BsiWI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstXI BstZ17I BtgZI ClaI CspCI DrdI
EagI EcoRI FseI FspI FspAI HaeII HpaI KasI
KpnI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI StuI SwaI TaqII Tth111I XbaI XhoI XmaI
ZraI