Restriction map for
pFN21AE5807
(Linear) (Six-base) MAPSORT of: KIEE5807.seq Check: 3824 from: 1 to: 4218
KIEE5807.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
Acc65I G'GTAC_C
Cuts at: 0 1341 4218
Size: 1341 2877
AclI AA'CG_TT
Cuts at: 0 1375 1533 3599 4218
Size: 1375 158 2066 619
Fragments arranged by size:
2066 1375 619 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 485 1048 1207 1261 1523 2645 2977
Size: 107 378 563 159 54 262 1122 332
Cuts at: 2977 3566 4218
Size: 589 652
Fragments arranged by size:
1122 652 589 563 378 332 262 159
107 54
AflII C'TTAA_G
Cuts at: 0 609 4218
Size: 609 3609
AflIII A'CryG_T
Cuts at: 0 69 4218
Size: 69 4149
AleI CACnn'nnGTG
Cuts at: 0 424 3847 4218
Size: 424 3423 371
Fragments arranged by size:
3423 424 371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 784 1271 4218
Size: 784 487 2947
Fragments arranged by size:
2947 784 487
AlwNI CAG_nnn'CTG
Cuts at: 0 312 999 1590 4218
Size: 312 687 591 2628
Fragments arranged by size:
2628 687 591 312
ApaI G_GGCC'C
Cuts at: 0 58 4218
Size: 58 4160
ApaLI G'TGCA_C
Cuts at: 0 3794 4218
Size: 3794 424
ApoI r'AATT_y
Cuts at: 0 370 491 523 619 640 2485 3120 3210
Size: 370 121 32 96 21 1845 635 90
Cuts at: 3210 3355 3460 3477 4218
Size: 145 105 17 741
Fragments arranged by size:
1845 741 635 370 145 121 105 96
90 32 21 17
AseI AT'TA_AT
Cuts at: 0 2152 4218
Size: 2152 2066
AvaI C'yCGr_G
Cuts at: 0 114 4218
Size: 114 4104
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3619 3652 4218
Size: 3619 33 566
Fragments arranged by size:
3619 566 33
BanI G'GyrC_C
Cuts at: 0 1341 4218
Size: 1341 2877
BanII G_rGCy'C
Cuts at: 0 58 1561 4218
Size: 58 1503 2657
Fragments arranged by size:
2657 1503 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 863 2735 3352 3808 4218
Size: 863 1872 617 456 410
Fragments arranged by size:
1872 863 617 456 410
BbvCI CC'TCA_GC
Cuts at: 0 238 2578 4218
Size: 238 2340 1640
Fragments arranged by size:
2340 1640 238
BciVI GTATCCnnnnn_n'
Cuts at: 0 3084 3227 4218
Size: 3084 143 991
Fragments arranged by size:
3084 991 143
BclI T'GATC_A
Cuts at: 0 2372 4218
Size: 2372 1846
BfrBI ATG'CAT
Cuts at: 0 2347 3396 4218
Size: 2347 1049 822
Fragments arranged by size:
2347 1049 822
BglII A'GATC_T
Cuts at: 0 414 1999 2443 4218
Size: 414 1585 444 1775
Fragments arranged by size:
1775 1585 444 414
BlpI GC'TnA_GC
Cuts at: 0 325 4218
Size: 325 3893
Bme1580I G_kGCm'C
Cuts at: 0 58 3798 4218
Size: 58 3740 420
Fragments arranged by size:
3740 420 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 291 2048 4218
Size: 291 1757 2170
Fragments arranged by size:
2170 1757 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 382 748 802 2018 2320 4218
Size: 334 48 366 54 1216 302 1898
Fragments arranged by size:
1898 1216 366 334 302 54 48
Bpu10I CC'TnA_GC
Cuts at: 0 238 2578 4218
Size: 238 2340 1640
Fragments arranged by size:
2340 1640 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1295 3658 4218
Size: 1295 2363 560
Fragments arranged by size:
2363 1295 560
BsaI GGTCTCn'nnnn_
Cuts at: 0 2203 4218
Size: 2203 2015
BsaAI yAC'GTr
Cuts at: 0 70 4218
Size: 70 4148
BsaHI Gr'CG_yC
Cuts at: 0 3938 4218
Size: 3938 280
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 74 104 4218
Size: 74 30 4114
Fragments arranged by size:
4114 74 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 298 355 745 2040 2337 3046 3667
Size: 226 72 57 390 1295 297 709 621
Cuts at: 3667 4218
Size: 551
Fragments arranged by size:
1295 709 621 551 390 297 226 72
57
BseYI C'CCAG_C
Cuts at: 0 39 51 1037 2591 3133 4218
Size: 39 12 986 1554 542 1085
Fragments arranged by size:
1554 1085 986 542 39 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 4218
Size: 744 3474
BsiHKAI G_wGCw'C
Cuts at: 0 1296 1561 3798 4218
Size: 1296 265 2237 420
Fragments arranged by size:
2237 1296 420 265
BsmI GAATG_Cn'
Cuts at: 0 2820 3003 3312 4218
Size: 2820 183 309 906
Fragments arranged by size:
2820 906 309 183
BsmBI CGTCTCn'nnnn_
Cuts at: 0 400 4218
Size: 400 3818
Bsp1286I G_dGCh'C
Cuts at: 0 58 1296 1561 3798 4218
Size: 58 1238 265 2237 420
Fragments arranged by size:
2237 1238 420 265 58
BspHI T'CATG_A
Cuts at: 0 1490 2805 4218
Size: 1490 1315 1413
Fragments arranged by size:
1490 1413 1315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 776 4218
Size: 776 3442
BsrDI GCAATG_nn'
Cuts at: 0 104 2568 3725 3766 3805 4218
Size: 104 2464 1157 41 39 413
Fragments arranged by size:
2464 1157 413 104 41 39
BsrFI r'CCGG_y
Cuts at: 0 124 194 4218
Size: 124 70 4024
Fragments arranged by size:
4024 124 70
BsrGI T'GTAC_A
Cuts at: 0 684 4091 4218
Size: 684 3407 127
Fragments arranged by size:
3407 684 127
BssHII G'CGCG_C
Cuts at: 0 242 4218
Size: 242 3976
BstAPI GCAn_nnn'nTGC
Cuts at: 0 999 4218
Size: 999 3219
BstEII G'GTnAC_C
Cuts at: 0 294 4218
Size: 294 3924
BstYI r'GATC_y
Cuts at: 0 414 1794 1999 2443 3669 4218
Size: 414 1380 205 444 1226 549
Fragments arranged by size:
1380 1226 549 444 414 205
Bsu36I CC'TnA_GG
Cuts at: 0 2320 4218
Size: 2320 1898
BtgI C'CryG_G
Cuts at: 0 248 3229 4218
Size: 248 2981 989
Fragments arranged by size:
2981 989 248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 304 1333 4218
Size: 304 1029 2885
Fragments arranged by size:
2885 1029 304
BtsI GCAGTG_nn'
Cuts at: 0 1764 2622 4218
Size: 1764 858 1596
Fragments arranged by size:
1764 1596 858
DraI TTT'AAA
Cuts at: 0 645 2490 2771 4218
Size: 645 1845 281 1447
Fragments arranged by size:
1845 1447 645 281
EaeI y'GGCC_r
Cuts at: 0 2426 3860 4218
Size: 2426 1434 358
Fragments arranged by size:
2426 1434 358
EarI CTCTTCn'nnn_
Cuts at: 0 314 692 1304 1571 1974 2360 3827 4218
Size: 314 378 612 267 403 386 1467 391
Fragments arranged by size:
1467 612 403 391 386 378 314 267
EcoICRI GAG'CTC
Cuts at: 0 1559 4218
Size: 1559 2659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 334 382 485 748 802 1048 1207
Size: 107 227 48 103 263 54 246 159
Cuts at: 1207 1261 1523 2018 2320 2645 2977 3566 4218
Size: 54 262 495 302 325 332 589 652
Fragments arranged by size:
652 589 495 332 325 302 263 262
246 227 159 107 103 54 54 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 355 2581 4218
Size: 337 18 2226 1637
Fragments arranged by size:
2226 1637 337 18
EcoO109I rG'GnC_Cy
Cuts at: 0 55 62 202 3783 4218
Size: 55 7 140 3581 435
Fragments arranged by size:
3581 435 140 55 7
EcoRI G'AATT_C
Cuts at: 0 619 4218
Size: 619 3599
EcoRV GAT'ATC
Cuts at: 0 3407 4218
Size: 3407 811
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1291 4135 4218
Size: 1291 2844 83
Fragments arranged by size:
2844 1291 83
FspI TGC'GCA
Cuts at: 0 103 4218
Size: 103 4115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1779 1811 2104 2136 3166 3198 3697 3729
Size: 1779 32 293 32 1030 32 499 32
Cuts at: 3729 4218
Size: 489
Fragments arranged by size:
1779 1030 499 489 293 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2892 4218
Size: 2892 1326
HindIII A'AGCT_T
Cuts at: 0 1365 3676 4218
Size: 1365 2311 542
Fragments arranged by size:
2311 1365 542
KpnI G_GTAC'C
Cuts at: 0 1345 4218
Size: 1345 2873
MfeI C'AATT_G
Cuts at: 0 3286 3572 4218
Size: 3286 286 646
Fragments arranged by size:
3286 646 286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2039 2069 2746 3581 4218
Size: 2039 30 677 835 637
Fragments arranged by size:
2039 835 677 637 30
MscI TGG'CCA
Cuts at: 0 2428 3862 4218
Size: 2428 1434 356
Fragments arranged by size:
2428 1434 356
MslI CAynn'nnrTG
Cuts at: 0 424 2437 3847 4201 4218
Size: 424 2013 1410 354 17
Fragments arranged by size:
2013 1410 424 354 17
MspA1I CmG'CkG
Cuts at: 0 51 309 758 1123 4218
Size: 51 258 449 365 3095
Fragments arranged by size:
3095 449 365 258 51
NaeI GCC'GGC
Cuts at: 0 126 196 4218
Size: 126 70 4022
Fragments arranged by size:
4022 126 70
NcoI C'CATG_G
Cuts at: 0 248 4218
Size: 248 3970
NgoMIV G'CCGG_C
Cuts at: 0 124 194 4218
Size: 124 70 4024
Fragments arranged by size:
4024 124 70
NsiI A_TGCA'T
Cuts at: 0 2349 3398 4218
Size: 2349 1049 820
Fragments arranged by size:
2349 1049 820
PflMI CCAn_nnn'nTGG
Cuts at: 0 73 3972 4218
Size: 73 3899 246
Fragments arranged by size:
3899 246 73
PmlI CAC'GTG
Cuts at: 0 70 4218
Size: 70 4148
PpuMI rG'GwC_Cy
Cuts at: 0 62 3783 4218
Size: 62 3721 435
Fragments arranged by size:
3721 435 62
PshAI GACnn'nnGTC
Cuts at: 0 2308 4218
Size: 2308 1910
PsiI TTA'TAA
Cuts at: 0 718 4218
Size: 718 3500
PspOMI G'GGCC_C
Cuts at: 0 54 4218
Size: 54 4164
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1904 1936 4218
Size: 1904 32 2282
Fragments arranged by size:
2282 1904 32
PstI C_TGCA'G
Cuts at: 0 995 1004 4218
Size: 995 9 3214
Fragments arranged by size:
3214 995 9
PvuII CAG'CTG
Cuts at: 0 51 309 1123 4218
Size: 51 258 814 3095
Fragments arranged by size:
3095 814 258 51
SacI G_AGCT'C
Cuts at: 0 1561 4218
Size: 1561 2657
SapI GCTCTTCn'nnn_
Cuts at: 0 314 692 1571 2360 4218
Size: 314 378 879 789 1858
Fragments arranged by size:
1858 879 789 378 314
ScaI AGT'ACT
Cuts at: 0 545 1094 2031 4151 4218
Size: 545 549 937 2120 67
Fragments arranged by size:
2120 937 549 545 67
SfcI C'TryA_G
Cuts at: 0 991 1000 1582 1696 1716 4218
Size: 991 9 582 114 20 2502
Fragments arranged by size:
2502 991 582 114 20 9
SmlI C'TyrA_G
Cuts at: 0 609 1310 3673 4218
Size: 609 701 2363 545
Fragments arranged by size:
2363 701 609 545
SpeI A'CTAG_T
Cuts at: 0 711 4179 4218
Size: 711 3468 39
Fragments arranged by size:
3468 711 39
SspI AAT'ATT
Cuts at: 0 2940 4019 4218
Size: 2940 1079 199
Fragments arranged by size:
2940 1079 199
StuI AGG'CCT
Cuts at: 0 112 4218
Size: 112 4106
StyI C'CwwG_G
Cuts at: 0 248 2477 3971 4218
Size: 248 2229 1494 247
Fragments arranged by size:
2229 1494 248 247
SwaI ATTT'AAAT
Cuts at: 0 2771 4218
Size: 2771 1447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 159 163 4218
Size: 159 4 4055
Fragments arranged by size:
4055 159 4
TatI w'GTAC_w
Cuts at: 0 90 543 684 1024 1092 1920 2029 2757
Size: 90 453 141 340 68 828 109 728
Cuts at: 2757 2903 2988 4091 4149 4218
Size: 146 85 1103 58 69
Fragments arranged by size:
1103 828 728 453 340 146 141 109
90 85 69 68 58
XbaI T'CTAG_A
Cuts at: 0 486 4218
Size: 486 3732
XmnI GAAnn'nnTTC
Cuts at: 0 619 1112 1274 4218
Size: 619 493 162 2944
Fragments arranged by size:
2944 619 493 162
Enzymes that do cut:
Acc65I AclI AcuI AflII AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI PflMI PmlI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuII SacI SapI ScaI
SfcI SmlI SpeI SspI StuI StyI SwaI TaqII
TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AgeI AhdI AloI AscI
AsiSI AvrII BamHI BbeI BcgI BglI BmgBI BmrI
BmtI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BssSI
BstBI BstXI BstZ17I ClaI CspCI DraIII DrdI EagI
EciI FseI FspAI HaeII HpaI KasI MluI NarI
NdeI NheI NotI NruI NspI PacI PasI PciI
PfoI PmeI PpiI PspXI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI TstI Tth111I XcmI XhoI XmaI
ZraI