Restriction map for
pFN21AE5805
(Linear) (Six-base) MAPSORT of: KIEE5805.seq Check: 3176 from: 1 to: 2997
KIEE5805.orf
With 182 enzymes: SgfI *
July 25, 2014 11:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 558 704 2997
Size: 558 146 2293
Fragments arranged by size:
2293 558 146
AccI GT'mk_AC
Cuts at: 0 284 2997
Size: 284 2713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 865 2587 2924 2997
Size: 26 839 1722 337 73
Fragments arranged by size:
1722 839 337 73 26
AleI CACnn'nnGTG
Cuts at: 0 2992 2997
Size: 2992 5
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1591 1623 2599 2631 2997
Size: 1591 32 976 32 366
Fragments arranged by size:
1591 976 366 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 573 684 2997
Size: 573 111 2313
Fragments arranged by size:
2313 573 111
ApaI G_GGCC'C
Cuts at: 0 79 123 406 2655 2997
Size: 79 44 283 2249 342
Fragments arranged by size:
2249 342 283 79 44
ApaLI G'TGCA_C
Cuts at: 0 322 832 2997
Size: 322 510 2165
Fragments arranged by size:
2165 510 322
ApoI r'AATT_y
Cuts at: 0 469 589 2997
Size: 469 120 2408
Fragments arranged by size:
2408 469 120
AvaI C'yCGr_G
Cuts at: 0 116 704 863 2997
Size: 116 588 159 2134
Fragments arranged by size:
2134 588 159 116
BamHI G'GATC_C
Cuts at: 0 535 2997
Size: 535 2462
BanI G'GyrC_C
Cuts at: 0 2283 2997
Size: 2283 714
BanII G_rGCy'C
Cuts at: 0 49 79 123 406 691 2018 2655 2893
Size: 49 30 44 283 285 1327 637 238
Cuts at: 2893 2997
Size: 104
Fragments arranged by size:
1327 637 285 283 238 104 49 44
30
BbsI GAAGACnn'nnnn_
Cuts at: 0 1305 2997
Size: 1305 1692
BbvCI CC'TCA_GC
Cuts at: 0 1255 2997
Size: 1255 1742
BciVI GTATCCnnnnn_n'
Cuts at: 0 545 2997
Size: 545 2452
BclI T'GATC_A
Cuts at: 0 2234 2997
Size: 2234 763
BglI GCCn_nnn'nGGC
Cuts at: 0 583 2707 2957 2997
Size: 583 2124 250 40
Fragments arranged by size:
2124 583 250 40
BglII A'GATC_T
Cuts at: 0 1781 2997
Size: 1781 1216
BlpI GC'TnA_GC
Cuts at: 0 43 685 1260 2029 2931 2997
Size: 43 642 575 769 902 66
Fragments arranged by size:
902 769 642 575 66 43
Bme1580I G_kGCm'C
Cuts at: 0 79 123 326 406 836 1434 2655 2997
Size: 79 44 203 80 430 598 1221 342
Fragments arranged by size:
1221 598 430 342 203 80 79 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 1393 2997
Size: 1393 1604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 520 741 1979 2156 2997
Size: 460 60 221 1238 177 841
Fragments arranged by size:
1238 841 460 221 177 60
Bpu10I CC'TnA_GC
Cuts at: 0 1255 2746 2997
Size: 1255 1491 251
Fragments arranged by size:
1491 1255 251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 526 1861 2845 2997
Size: 85 441 1335 984 152
Fragments arranged by size:
1335 984 441 152 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 2119 2997
Size: 2119 878
BsaWI w'CCGG_w
Cuts at: 0 308 548 2217 2997
Size: 308 240 1669 780
Fragments arranged by size:
1669 780 308 240
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 35 739 754 769 784 1121 1151
Size: 5 30 704 15 15 15 337 30
Cuts at: 1151 1591 1621 2479 2509 2527 2557 2599 2629
Size: 440 30 858 30 18 30 42 30
Cuts at: 2629 2997
Size: 368
Fragments arranged by size:
858 704 440 368 337 42 30 30
30 30 30 30 18 15 15 15
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 457 460 2290 2860 2901 2997
Size: 457 3 1830 570 41 96
Fragments arranged by size:
1830 570 457 96 41 3
BseYI C'CCAG_C
Cuts at: 0 397 420 1086 1177 2955 2997
Size: 397 23 666 91 1778 42
Fragments arranged by size:
1778 666 397 91 42 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2503 2997
Size: 2503 494
BsiEI CG_ry'CG
Cuts at: 0 2362 2997
Size: 2362 635
BsiHKAI G_wGCw'C
Cuts at: 0 181 326 479 691 836 1995 2018 2997
Size: 181 145 153 212 145 1159 23 979
Fragments arranged by size:
1159 979 212 181 153 145 145 23
BsmI GAATG_Cn'
Cuts at: 0 1209 2021 2997
Size: 1209 812 976
Fragments arranged by size:
1209 976 812
BsmBI CGTCTCn'nnnn_
Cuts at: 0 369 2349 2997
Size: 369 1980 648
Fragments arranged by size:
1980 648 369
Bsp1286I G_dGCh'C
Cuts at: 0 49 79 123 181 326 406 479 691
Size: 49 30 44 58 145 80 73 212
Cuts at: 691 836 1434 1995 2018 2655 2893 2997
Size: 145 598 561 23 637 238 104
Fragments arranged by size:
637 598 561 238 212 145 145 104
80 73 58 49 44 30 23
BspEI T'CCGG_A
Cuts at: 0 548 2217 2997
Size: 548 1669 780
Fragments arranged by size:
1669 780 548
BspHI T'CATG_A
Cuts at: 0 933 1619 2997
Size: 933 686 1378
Fragments arranged by size:
1378 933 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 558 704 2612 2997
Size: 558 146 1908 385
Fragments arranged by size:
1908 558 385 146
BsrBI CCG'CTC
Cuts at: 0 429 1684 2997
Size: 429 1255 1313
Fragments arranged by size:
1313 1255 429
BsrDI GCAATG_nn'
Cuts at: 0 650 2450 2997
Size: 650 1800 547
Fragments arranged by size:
1800 650 547
BsrFI r'CCGG_y
Cuts at: 0 38 2877 2997
Size: 38 2839 120
Fragments arranged by size:
2839 120 38
BsrGI T'GTAC_A
Cuts at: 0 776 2997
Size: 776 2221
BssHII G'CGCG_C
Cuts at: 0 1000 2997
Size: 1000 1997
BssSI C'ACGA_G
Cuts at: 0 835 1756 2734 2755 2997
Size: 835 921 978 21 242
Fragments arranged by size:
978 921 835 242 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 857 2997
Size: 857 2140
BstBI TT'CG_AA
Cuts at: 0 2109 2158 2674 2997
Size: 2109 49 516 323
Fragments arranged by size:
2109 516 323 49
BstEII G'GTnAC_C
Cuts at: 0 1588 1972 2997
Size: 1588 384 1025
Fragments arranged by size:
1588 1025 384
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1353 2174 2997
Size: 1353 821 823
Fragments arranged by size:
1353 823 821
BstYI r'GATC_y
Cuts at: 0 150 535 839 1781 2997
Size: 150 385 304 942 1216
Fragments arranged by size:
1216 942 385 304 150
Bsu36I CC'TnA_GG
Cuts at: 0 50 2997
Size: 50 2947
BtgI C'CryG_G
Cuts at: 0 562 2647 2783 2997
Size: 562 2085 136 214
Fragments arranged by size:
2085 562 214 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1556 2997
Size: 1556 1441
BtsI GCAGTG_nn'
Cuts at: 0 100 2179 2997
Size: 100 2079 818
Fragments arranged by size:
2079 818 100
DraIII CAC_nnn'GTG
Cuts at: 0 1217 2997
Size: 1217 1780
DrdI GACnn_nn'nnGTC
Cuts at: 0 95 2997
Size: 95 2902
EaeI y'GGCC_r
Cuts at: 0 156 345 575 1796 2359 2997
Size: 156 189 230 1221 563 638
Fragments arranged by size:
1221 638 563 230 189 156
EagI C'GGCC_G
Cuts at: 0 2359 2997
Size: 2359 638
EarI CTCTTCn'nnn_
Cuts at: 0 935 1360 1409 1841 1979 2266 2809 2997
Size: 935 425 49 432 138 287 543 188
Fragments arranged by size:
935 543 432 425 287 188 138 49
EcoICRI GAG'CTC
Cuts at: 0 689 2016 2997
Size: 689 1327 981
Fragments arranged by size:
1327 981 689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 460 520 741 865 1979 2156 2587
Size: 26 434 60 221 124 1114 177 431
Cuts at: 2587 2924 2997
Size: 337 73
Fragments arranged by size:
1114 434 431 337 221 177 124 73
60 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 53 442 2842 2997
Size: 53 389 2400 155
Fragments arranged by size:
2400 389 155 53
EcoO109I rG'GnC_Cy
Cuts at: 0 75 76 112 120 402 1398 2178 2652
Size: 75 1 36 8 282 996 780 474
Cuts at: 2652 2997
Size: 345
Fragments arranged by size:
996 780 474 345 282 75 36 8
1
EcoRI G'AATT_C
Cuts at: 0 589 2997
Size: 589 2408
EcoRV GAT'ATC
Cuts at: 0 2385 2997
Size: 2385 612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 816 2997
Size: 816 2181
HaeII r_GCGC'y
Cuts at: 0 1793 1854 2997
Size: 1793 61 1143
Fragments arranged by size:
1793 1143 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 214 246 1647 1679 2392 2424
Size: 96 32 86 32 1401 32 713 32
Cuts at: 2424 2997
Size: 573
Fragments arranged by size:
1401 713 573 96 86 32 32 32
32
HincII GTy'rAC
Cuts at: 0 285 2532 2997
Size: 285 2247 465
Fragments arranged by size:
2247 465 285
HindIII A'AGCT_T
Cuts at: 0 1865 2997
Size: 1865 1132
MfeI C'AATT_G
Cuts at: 0 1160 2997
Size: 1160 1837
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 1050 2489 2997
Size: 167 883 1439 508
Fragments arranged by size:
1439 883 508 167
MscI TGG'CCA
Cuts at: 0 158 347 577 1798 2997
Size: 158 189 230 1221 1199
Fragments arranged by size:
1221 1199 230 189 158
MslI CAynn'nnrTG
Cuts at: 0 263 938 2038 2230 2317 2990 2992 2997
Size: 263 675 1100 192 87 673 2 5
Fragments arranged by size:
1100 675 673 263 192 87 5 2
MspA1I CmG'CkG
Cuts at: 0 462 2565 2955 2997
Size: 462 2103 390 42
Fragments arranged by size:
2103 462 390 42
NaeI GCC'GGC
Cuts at: 0 40 2879 2997
Size: 40 2839 118
Fragments arranged by size:
2839 118 40
NcoI C'CATG_G
Cuts at: 0 2647 2997
Size: 2647 350
NgoMIV G'CCGG_C
Cuts at: 0 38 2877 2997
Size: 38 2839 120
Fragments arranged by size:
2839 120 38
NspI r_CATG'y
Cuts at: 0 258 2997
Size: 258 2739
PflMI CCAn_nnn'nTGG
Cuts at: 0 354 1515 2899 2997
Size: 354 1161 1384 98
Fragments arranged by size:
1384 1161 354 98
PfoI T'CCnGG_A
Cuts at: 0 785 1352 1772 2997
Size: 785 567 420 1225
Fragments arranged by size:
1225 785 567 420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1243 1275 1591 1623 2599 2631 2997
Size: 1243 32 316 32 976 32 366
Fragments arranged by size:
1243 976 366 316 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 112 1398 2178 2997
Size: 112 1286 780 819
Fragments arranged by size:
1286 819 780 112
PspOMI G'GGCC_C
Cuts at: 0 75 119 402 2651 2997
Size: 75 44 283 2249 346
Fragments arranged by size:
2249 346 283 75 44
PstI C_TGCA'G
Cuts at: 0 134 680 701 1680 2623 2997
Size: 134 546 21 979 943 374
Fragments arranged by size:
979 943 546 374 134 21
PvuII CAG'CTG
Cuts at: 0 2565 2955 2997
Size: 2565 390 42
Fragments arranged by size:
2565 390 42
SacI G_AGCT'C
Cuts at: 0 691 2018 2997
Size: 691 1327 979
Fragments arranged by size:
1327 979 691
SalI G'TCGA_C
Cuts at: 0 283 2997
Size: 283 2714
SapI GCTCTTCn'nnn_
Cuts at: 0 1841 2809 2997
Size: 1841 968 188
Fragments arranged by size:
1841 968 188
ScaI AGT'ACT
Cuts at: 0 2494 2683 2997
Size: 2494 189 314
Fragments arranged by size:
2494 314 189
SfcI C'TryA_G
Cuts at: 0 130 676 697 1372 1676 2187 2499 2619
Size: 130 546 21 675 304 511 312 120
Cuts at: 2619 2997
Size: 378
Fragments arranged by size:
675 546 511 378 312 304 130 120
21
SmaI CCC'GGG
Cuts at: 0 118 2997
Size: 118 2879
SmlI C'TyrA_G
Cuts at: 0 100 505 1876 2860 2997
Size: 100 405 1371 984 137
Fragments arranged by size:
1371 984 405 137 100
SphI G_CATG'C
Cuts at: 0 258 2997
Size: 258 2739
StuI AGG'CCT
Cuts at: 0 1449 1944 2997
Size: 1449 495 1053
Fragments arranged by size:
1449 1053 495
StyI C'CwwG_G
Cuts at: 0 233 333 2647 2997
Size: 233 100 2314 350
Fragments arranged by size:
2314 350 233 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2143 2997
Size: 2143 854
TatI w'GTAC_w
Cuts at: 0 291 776 2222 2492 2681 2997
Size: 291 485 1446 270 189 316
Fragments arranged by size:
1446 485 316 291 270 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1121 1153 1203 1235 2527 2559 2997
Size: 1121 32 50 32 1292 32 438
Fragments arranged by size:
1292 1121 438 50 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 166 340 876 1908 2997
Size: 166 174 536 1032 1089
Fragments arranged by size:
1089 1032 536 174 166
XmaI C'CCGG_G
Cuts at: 0 116 2997
Size: 116 2881
XmnI GAAnn'nnTTC
Cuts at: 0 1172 1207 2997
Size: 1172 35 1790
Fragments arranged by size:
1790 1172 35
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SalI
SapI ScaI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BfrBI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsiWI
BstZ17I ClaI CspCI DraI EciI FseI FspI FspAI
HpaI KasI KpnI MluI NarI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI ZraI