Restriction map for
pFN21AE5792
(Linear) (Six-base) MAPSORT of: KIEE5792.seq Check: 3891 from: 1 to: 1968
KIEE5792.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AccI GT'mk_AC
Cuts at: 0 1232 1597 1968
Size: 1232 365 371
Fragments arranged by size:
1232 371 365
AclI AA'CG_TT
Cuts at: 0 335 1968
Size: 335 1633
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 440 1075 1829 1968
Size: 416 24 635 754 139
Fragments arranged by size:
754 635 416 139 24
AflIII A'CryG_T
Cuts at: 0 1531 1968
Size: 1531 437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1043 1968
Size: 1043 925
AlwNI CAG_nnn'CTG
Cuts at: 0 234 1968
Size: 234 1734
ApoI r'AATT_y
Cuts at: 0 772 895 1151 1813 1968
Size: 772 123 256 662 155
Fragments arranged by size:
772 662 256 155 123
AvaI C'yCGr_G
Cuts at: 0 1677 1968
Size: 1677 291
BanII G_rGCy'C
Cuts at: 0 1653 1968
Size: 1653 315
BbsI GAAGACnn'nnnn_
Cuts at: 0 1195 1316 1565 1847 1968
Size: 1195 121 249 282 121
Fragments arranged by size:
1195 282 249 121 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 442 476 1968
Size: 442 34 1492
Fragments arranged by size:
1492 442 34
BfrBI ATG'CAT
Cuts at: 0 1502 1968
Size: 1502 466
BglII A'GATC_T
Cuts at: 0 1358 1796 1968
Size: 1358 438 172
Fragments arranged by size:
1358 438 172
BmrI ACTGGGnnnn_n'
Cuts at: 0 147 1968
Size: 147 1821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1424 1968
Size: 1424 544
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1968
Size: 704 1264
BsaBI GATnn'nnATC
Cuts at: 0 165 1409 1968
Size: 165 1244 559
Fragments arranged by size:
1244 559 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1199 1366 1968
Size: 1003 196 167 602
Fragments arranged by size:
1003 602 196 167
BsiHKAI G_wGCw'C
Cuts at: 0 354 1968
Size: 354 1614
Bsp1286I G_dGCh'C
Cuts at: 0 354 1653 1968
Size: 354 1299 315
Fragments arranged by size:
1299 354 315
BsrDI GCAATG_nn'
Cuts at: 0 1037 1968
Size: 1037 931
BstYI r'GATC_y
Cuts at: 0 1358 1796 1968
Size: 1358 438 172
Fragments arranged by size:
1358 438 172
BstZ17I GTA'TAC
Cuts at: 0 1598 1968
Size: 1598 370
BtgI C'CryG_G
Cuts at: 0 869 1968
Size: 869 1099
BtsI GCAGTG_nn'
Cuts at: 0 961 1968
Size: 961 1007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6 41 1968
Size: 6 35 1927
Fragments arranged by size:
1927 35 6
DraI TTT'AAA
Cuts at: 0 777 1968
Size: 777 1191
EaeI y'GGCC_r
Cuts at: 0 1140 1904 1968
Size: 1140 764 64
Fragments arranged by size:
1140 764 64
EarI CTCTTCn'nnn_
Cuts at: 0 269 335 1968
Size: 269 66 1633
Fragments arranged by size:
1633 269 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1968
Size: 19 1949
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 440 1075 1424 1829 1968
Size: 416 24 635 349 405 139
Fragments arranged by size:
635 416 405 349 139 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1324 1968
Size: 1324 644
EcoO109I rG'GnC_Cy
Cuts at: 0 231 1968
Size: 231 1737
EcoRI G'AATT_C
Cuts at: 0 1813 1968
Size: 1813 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 336 368 685 717 830 862 1225 1257
Size: 336 32 317 32 113 32 363 32
Cuts at: 1257 1422 1454 1552 1584 1968
Size: 165 32 98 32 384
Fragments arranged by size:
384 363 336 317 165 113 98 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1233 1968
Size: 1233 735
HindIII A'AGCT_T
Cuts at: 0 299 944 1968
Size: 299 645 1024
Fragments arranged by size:
1024 645 299
MfeI C'AATT_G
Cuts at: 0 1025 1968
Size: 1025 943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1828 1968
Size: 1828 140
MscI TGG'CCA
Cuts at: 0 1142 1906 1968
Size: 1142 764 62
Fragments arranged by size:
1142 764 62
MslI CAynn'nnrTG
Cuts at: 0 932 1968
Size: 932 1036
MspA1I CmG'CkG
Cuts at: 0 73 1968
Size: 73 1895
NcoI C'CATG_G
Cuts at: 0 869 1968
Size: 869 1099
NsiI A_TGCA'T
Cuts at: 0 1504 1968
Size: 1504 464
NspI r_CATG'y
Cuts at: 0 80 1337 1502 1535 1765 1968
Size: 80 1257 165 33 230 203
Fragments arranged by size:
1257 230 203 165 80 33
PasI CC'CwG_GG
Cuts at: 0 1158 1968
Size: 1158 810
PciI A'CATG_T
Cuts at: 0 1531 1968
Size: 1531 437
PfoI T'CCnGG_A
Cuts at: 0 1790 1881 1968
Size: 1790 91 87
Fragments arranged by size:
1790 91 87
PpuMI rG'GwC_Cy
Cuts at: 0 231 1968
Size: 231 1737
PsiI TTA'TAA
Cuts at: 0 37 1968
Size: 37 1931
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 809 841 1968
Size: 809 32 1127
Fragments arranged by size:
1127 809 32
PstI C_TGCA'G
Cuts at: 0 359 1968
Size: 359 1609
PvuII CAG'CTG
Cuts at: 0 73 1968
Size: 73 1895
SalI G'TCGA_C
Cuts at: 0 1231 1968
Size: 1231 737
ScaI AGT'ACT
Cuts at: 0 216 1373 1968
Size: 216 1157 595
Fragments arranged by size:
1157 595 216
SfcI C'TryA_G
Cuts at: 0 355 608 1167 1968
Size: 355 253 559 801
Fragments arranged by size:
801 559 355 253
SmlI C'TyrA_G
Cuts at: 0 719 1968
Size: 719 1249
SphI G_CATG'C
Cuts at: 0 1765 1968
Size: 1765 203
SspI AAT'ATT
Cuts at: 0 690 1297 1968
Size: 690 607 671
Fragments arranged by size:
690 671 607
StyI C'CwwG_G
Cuts at: 0 869 1143 1968
Size: 869 274 825
Fragments arranged by size:
869 825 274
TatI w'GTAC_w
Cuts at: 0 214 1014 1215 1371 1968
Size: 214 800 201 156 597
Fragments arranged by size:
800 597 214 201 156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 1643 1968
Size: 321 1322 325
Fragments arranged by size:
1322 325 321
XmnI GAAnn'nnTTC
Cuts at: 0 284 824 1585 1968
Size: 284 540 761 383
Fragments arranged by size:
761 540 383 284
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI AlwNI ApoI AvaI
BanII BbsI BcgI BfrBI BglII BmrI BpmI BpuEI
BsaBI BsgI BsiHKAI Bsp1286I BsrDI BstYI BstZ17I BtgI
BtsI CspCI DraI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NcoI NsiI NspI PasI PciI PfoI
PpuMI PsiI PsrI PstI PvuII SalI ScaI SfcI
SmlI SphI SspI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BciVI BclI BglI BlpI
Bme1580I BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI Bsu36I BtgZI ClaI DraIII
DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI
PpiI PshAI PspOMI PspXI PvuI RsrII SacI SacII
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI ZraI