Restriction map for
pFN21AE5787
(Linear) (Six-base) MAPSORT of: KIEE5787.seq Check: 1187 from: 1 to: 2253
KIEE5787.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AatII G_ACGT'C
Cuts at: 0 2192 2253
Size: 2192 61
AccI GT'mk_AC
Cuts at: 0 1445 2253
Size: 1445 808
AclI AA'CG_TT
Cuts at: 0 497 2253
Size: 497 1756
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 2253
Size: 983 1270
AfeI AGC'GCT
Cuts at: 0 490 2253
Size: 490 1763
AlwNI CAG_nnn'CTG
Cuts at: 0 1521 2253
Size: 1521 732
ApaI G_GGCC'C
Cuts at: 0 51 402 558 1792 2253
Size: 51 351 156 1234 461
Fragments arranged by size:
1234 461 351 156 51
ApoI r'AATT_y
Cuts at: 0 1393 1702 1975 2253
Size: 1393 309 273 278
Fragments arranged by size:
1393 309 278 273
AseI AT'TA_AT
Cuts at: 0 2195 2253
Size: 2195 58
AvrII C'CTAG_G
Cuts at: 0 60 2253
Size: 60 2193
BanII G_rGCy'C
Cuts at: 0 51 402 541 558 1792 1820 2253
Size: 51 351 139 17 1234 28 433
Fragments arranged by size:
1234 433 351 139 51 28 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 98 2192 2253
Size: 98 2094 61
Fragments arranged by size:
2094 98 61
BfrBI ATG'CAT
Cuts at: 0 228 1203 2253
Size: 228 975 1050
Fragments arranged by size:
1050 975 228
BglI GCCn_nnn'nGGC
Cuts at: 0 28 2253
Size: 28 2225
BglII A'GATC_T
Cuts at: 0 645 2253
Size: 645 1608
Bme1580I G_kGCm'C
Cuts at: 0 51 402 558 1792 2253
Size: 51 351 156 1234 461
Fragments arranged by size:
1234 461 351 156 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 2036 2253
Size: 2036 217
BmtI G_CTAG'C
Cuts at: 0 844 2253
Size: 844 1409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1017 2253
Size: 1017 1236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1017 1099 1687 2253
Size: 1017 82 588 566
Fragments arranged by size:
1017 588 566 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 1573 2253
Size: 1573 680
BsaHI Gr'CG_yC
Cuts at: 0 2010 2189 2253
Size: 2010 179 64
Fragments arranged by size:
2010 179 64
BsaWI w'CCGG_w
Cuts at: 0 1820 2253
Size: 1820 433
BseYI C'CCAG_C
Cuts at: 0 546 2253
Size: 546 1707
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 1655 2253
Size: 440 1215 598
Fragments arranged by size:
1215 598 440
BsiHKAI G_wGCw'C
Cuts at: 0 1845 1899 2253
Size: 1845 54 354
Fragments arranged by size:
1845 354 54
Bsp1286I G_dGCh'C
Cuts at: 0 51 402 541 558 1792 1820 1845 1899
Size: 51 351 139 17 1234 28 25 54
Cuts at: 1899 2253
Size: 354
Fragments arranged by size:
1234 354 351 139 54 51 28 25
17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 69 1403 2144 2253
Size: 69 1334 741 109
Fragments arranged by size:
1334 741 109 69
BsrDI GCAATG_nn'
Cuts at: 0 1367 2253
Size: 1367 886
BsrFI r'CCGG_y
Cuts at: 0 746 2253
Size: 746 1507
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1746 2253
Size: 1746 507
BstBI TT'CG_AA
Cuts at: 0 849 2253
Size: 849 1404
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 2253
Size: 618 1635
BstYI r'GATC_y
Cuts at: 0 645 1428 2253
Size: 645 783 825
Fragments arranged by size:
825 783 645
BstZ17I GTA'TAC
Cuts at: 0 1446 2253
Size: 1446 807
BtgI C'CryG_G
Cuts at: 0 272 611 2013 2253
Size: 272 339 1402 240
Fragments arranged by size:
1402 339 272 240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 371 406 2253
Size: 371 35 1847
Fragments arranged by size:
1847 371 35
DraI TTT'AAA
Cuts at: 0 633 836 1074 1440 2253
Size: 633 203 238 366 813
Fragments arranged by size:
813 633 366 238 203
DraIII CAC_nnn'GTG
Cuts at: 0 329 2253
Size: 329 1924
EaeI y'GGCC_r
Cuts at: 0 734 2253
Size: 734 1519
EarI CTCTTCn'nnn_
Cuts at: 0 846 2253
Size: 846 1407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1017 1099 1687 2253
Size: 983 34 82 588 566
Fragments arranged by size:
983 588 566 82 34
EcoO109I rG'GnC_Cy
Cuts at: 0 47 48 398 399 554 555 1788 1789
Size: 47 1 350 1 155 1 1233 1
Cuts at: 1789 2253
Size: 464
Fragments arranged by size:
1233 464 350 155 47 1 1 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1915 2253
Size: 1915 338
HaeII r_GCGC'y
Cuts at: 0 492 899 2253
Size: 492 407 1354
Fragments arranged by size:
1354 492 407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 459 491 2253
Size: 459 32 1762
Fragments arranged by size:
1762 459 32
HincII GTy'rAC
Cuts at: 0 2221 2253
Size: 2221 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 423 566 2253
Size: 392 31 143 1687
Fragments arranged by size:
1687 392 143 31
MspA1I CmG'CkG
Cuts at: 0 718 1310 2253
Size: 718 592 943
Fragments arranged by size:
943 718 592
NcoI C'CATG_G
Cuts at: 0 272 611 2013 2253
Size: 272 339 1402 240
Fragments arranged by size:
1402 339 272 240
NheI G'CTAG_C
Cuts at: 0 840 2253
Size: 840 1413
NsiI A_TGCA'T
Cuts at: 0 230 1205 2253
Size: 230 975 1048
Fragments arranged by size:
1048 975 230
NspI r_CATG'y
Cuts at: 0 373 1203 1207 2253
Size: 373 830 4 1046
Fragments arranged by size:
1046 830 373 4
PfoI T'CCnGG_A
Cuts at: 0 1664 2253
Size: 1664 589
PshAI GACnn'nnGTC
Cuts at: 0 686 2253
Size: 686 1567
PspOMI G'GGCC_C
Cuts at: 0 47 398 554 1788 2253
Size: 47 351 156 1234 465
Fragments arranged by size:
1234 465 351 156 47
PstI C_TGCA'G
Cuts at: 0 21 563 2253
Size: 21 542 1690
Fragments arranged by size:
1690 542 21
PvuII CAG'CTG
Cuts at: 0 718 2253
Size: 718 1535
SapI GCTCTTCn'nnn_
Cuts at: 0 846 2253
Size: 846 1407
SfcI C'TryA_G
Cuts at: 0 17 559 1754 2213 2253
Size: 17 542 1195 459 40
Fragments arranged by size:
1195 542 459 40 17
SphI G_CATG'C
Cuts at: 0 1203 1207 2253
Size: 1203 4 1046
Fragments arranged by size:
1203 1046 4
SspI AAT'ATT
Cuts at: 0 144 345 1731 2253
Size: 144 201 1386 522
Fragments arranged by size:
1386 522 201 144
StuI AGG'CCT
Cuts at: 0 94 1064 2253
Size: 94 970 1189
Fragments arranged by size:
1189 970 94
StyI C'CwwG_G
Cuts at: 0 60 272 611 1910 2013 2253
Size: 60 212 339 1299 103 240
Fragments arranged by size:
1299 339 240 212 103 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 545 1067 2253
Size: 545 522 1186
Fragments arranged by size:
1186 545 522
ZraI GAC'GTC
Cuts at: 0 2190 2253
Size: 2190 63
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AlwNI ApaI ApoI
AseI AvrII BanII BbsI BfrBI BglI BglII Bme1580I
BmrI BmtI BplI BpmI BsaI BsaHI BsaWI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI
BstXI BstYI BstZ17I BtgI CspCI DraI DraIII EaeI
EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII MmeI
MspA1I NcoI NheI NsiI NspI PfoI PshAI PspOMI
PstI PvuII SapI SfcI SphI SspI StuI StyI
TaqII ZraI
Enzymes that do not cut:
AarI Acc65I AflII AflIII AgeI AhdI AleI AlfI
AloI ApaLI AscI AsiSI AvaI BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BclI BlpI BmgBI Bpu10I
BpuEI BsaAI BsaBI BsaXI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstEII
Bsu36I BtgZI BtsI ClaI DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NdeI NgoMIV NotI NruI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI