Restriction map for
pFN21AE5785
(Linear) (Six-base) MAPSORT of: KIEE5785.seq Check: 1580 from: 1 to: 2289
KIEE5785.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 366 1677 2289
Size: 366 1311 612
Fragments arranged by size:
1311 612 366
Acc65I G'GTAC_C
Cuts at: 0 1886 2289
Size: 1886 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 951 1857 2289
Size: 925 26 906 432
Fragments arranged by size:
925 906 432 26
AleI CACnn'nnGTG
Cuts at: 0 1228 1442 2289
Size: 1228 214 847
Fragments arranged by size:
1228 847 214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1834 2289
Size: 1834 455
AlwNI CAG_nnn'CTG
Cuts at: 0 234 1683 2086 2289
Size: 234 1449 403 203
Fragments arranged by size:
1449 403 234 203
ApaI G_GGCC'C
Cuts at: 0 1965 2289
Size: 1965 324
ApoI r'AATT_y
Cuts at: 0 1122 2289
Size: 1122 1167
AvaI C'yCGr_G
Cuts at: 0 362 699 921 1799 2289
Size: 362 337 222 878 490
Fragments arranged by size:
878 490 362 337 222
BanI G'GyrC_C
Cuts at: 0 1886 2289
Size: 1886 403
BanII G_rGCy'C
Cuts at: 0 352 1663 1925 1965 2289
Size: 352 1311 262 40 324
Fragments arranged by size:
1311 352 324 262 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 16 279 375 1425 2144 2289
Size: 16 263 96 1050 719 145
Fragments arranged by size:
1050 719 263 145 96 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2239 2273 2289
Size: 2239 34 16
Fragments arranged by size:
2239 34 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 1419 2289
Size: 1419 870
BglI GCCn_nnn'nGGC
Cuts at: 0 367 2289
Size: 367 1922
BglII A'GATC_T
Cuts at: 0 1335 1387 2289
Size: 1335 52 902
Fragments arranged by size:
1335 902 52
BlpI GC'TnA_GC
Cuts at: 0 1822 2289
Size: 1822 467
Bme1580I G_kGCm'C
Cuts at: 0 144 920 1552 1655 1965 2247 2289
Size: 144 776 632 103 310 282 42
Fragments arranged by size:
776 632 310 282 144 103 42
BmgBI CAC'GTC
Cuts at: 0 462 2289
Size: 462 1827
BmrI ACTGGGnnnn_n'
Cuts at: 0 131 831 1436 1520 1620 1919 2289
Size: 131 700 605 84 100 299 370
Fragments arranged by size:
700 605 370 299 131 100 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1624 1677 1726 1853 2289
Size: 850 774 53 49 127 436
Fragments arranged by size:
850 774 436 127 53 49
Bpu10I CC'TnA_GC
Cuts at: 0 187 1664 2289
Size: 187 1477 625
Fragments arranged by size:
1477 625 187
BsaI GGTCTCn'nnnn_
Cuts at: 0 1993 2289
Size: 1993 296
BsaAI yAC'GTr
Cuts at: 0 726 1230 1276 2289
Size: 726 504 46 1013
Fragments arranged by size:
1013 726 504 46
BsaHI Gr'CG_yC
Cuts at: 0 504 2289
Size: 504 1785
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 624 654 823 853 2289
Size: 624 30 169 30 1436
Fragments arranged by size:
1436 624 169 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 211 984 1729 1930 1933 2289
Size: 16 195 773 745 201 3 356
Fragments arranged by size:
773 745 356 201 195 16 3
BseYI C'CCAG_C
Cuts at: 0 103 143 227 234 401 706 824 1128
Size: 103 40 84 7 167 305 118 304
Cuts at: 1128 1877 1904 2191 2289
Size: 749 27 287 98
Fragments arranged by size:
749 305 304 287 167 118 103 98
84 40 27 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 2289
Size: 703 1586
BsiHKAI G_wGCw'C
Cuts at: 0 26 1541 1670 2289
Size: 26 1515 129 619
Fragments arranged by size:
1515 619 129 26
BsmI GAATG_Cn'
Cuts at: 0 1027 1492 2289
Size: 1027 465 797
Fragments arranged by size:
1027 797 465
Bsp1286I G_dGCh'C
Cuts at: 0 26 144 352 920 1541 1552 1655 1663
Size: 26 118 208 568 621 11 103 8
Cuts at: 1663 1670 1925 1965 2247 2289
Size: 7 255 40 282 42
Fragments arranged by size:
621 568 282 255 208 118 103 42
40 26 11 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 366 1677 2289
Size: 366 1311 612
Fragments arranged by size:
1311 612 366
BsrDI GCAATG_nn'
Cuts at: 0 117 1142 2003 2289
Size: 117 1025 861 286
Fragments arranged by size:
1025 861 286 117
BsrFI r'CCGG_y
Cuts at: 0 847 2289
Size: 847 1442
BssSI C'ACGA_G
Cuts at: 0 19 1732 2289
Size: 19 1713 557
Fragments arranged by size:
1713 557 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1674 1683 2289
Size: 1674 9 606
Fragments arranged by size:
1674 606 9
BstEII G'GTnAC_C
Cuts at: 0 1689 1792 2289
Size: 1689 103 497
Fragments arranged by size:
1689 497 103
BstXI CCAn_nnnn'nTGG
Cuts at: 0 574 1230 1444 2191 2289
Size: 574 656 214 747 98
Fragments arranged by size:
747 656 574 214 98
BstYI r'GATC_y
Cuts at: 0 1043 1335 1387 1584 2289
Size: 1043 292 52 197 705
Fragments arranged by size:
1043 705 292 197 52
BtgI C'CryG_G
Cuts at: 0 635 645 1197 1472 1643 1859 2268 2289
Size: 635 10 552 275 171 216 409 21
Fragments arranged by size:
635 552 409 275 216 171 21 10
BtsI GCAGTG_nn'
Cuts at: 0 433 2289
Size: 433 1856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 574 609 660 695 2289
Size: 574 35 51 35 1594
Fragments arranged by size:
1594 574 51 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 726 2289
Size: 726 1563
DrdI GACnn_nn'nnGTC
Cuts at: 0 283 2289
Size: 283 2006
EaeI y'GGCC_r
Cuts at: 0 35 162 374 1106 1220 1766 2289
Size: 35 127 212 732 114 546 523
Fragments arranged by size:
732 546 523 212 127 114 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 925 951 1624 1677 1726 1853 1857
Size: 850 75 26 673 53 49 127 4
Cuts at: 1857 2289
Size: 432
Fragments arranged by size:
850 673 432 127 75 53 49 26
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1096 2227 2289
Size: 1096 1131 62
Fragments arranged by size:
1131 1096 62
EcoO109I rG'GnC_Cy
Cuts at: 0 484 756 893 958 1260 1308 1962 2195
Size: 484 272 137 65 302 48 654 233
Cuts at: 2195 2289
Size: 94
Fragments arranged by size:
654 484 302 272 233 137 94 65
48
FseI GG_CCGG'CC
Cuts at: 0 851 2289
Size: 851 1438
HaeII r_GCGC'y
Cuts at: 0 707 1571 1745 2289
Size: 707 864 174 544
Fragments arranged by size:
864 707 544 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 624 656 1327 1359 2289
Size: 624 32 671 32 930
Fragments arranged by size:
930 671 624 32 32
HincII GTy'rAC
Cuts at: 0 2208 2289
Size: 2208 81
KpnI G_GTAC'C
Cuts at: 0 1890 2289
Size: 1890 399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2139 2289
Size: 2139 150
MscI TGG'CCA
Cuts at: 0 164 376 1108 1222 1768 2289
Size: 164 212 732 114 546 521
Fragments arranged by size:
732 546 521 212 164 114
MslI CAynn'nnrTG
Cuts at: 0 1228 1442 1648 1816 2289
Size: 1228 214 206 168 473
Fragments arranged by size:
1228 473 214 206 168
MspA1I CmG'CkG
Cuts at: 0 174 231 306 773 828 1674 1842 1877
Size: 174 57 75 467 55 846 168 35
Cuts at: 1877 1893 1908 2038 2289
Size: 16 15 130 251
Fragments arranged by size:
846 467 251 174 168 130 75 57
55 35 16 15
NaeI GCC'GGC
Cuts at: 0 849 2289
Size: 849 1440
NcoI C'CATG_G
Cuts at: 0 1643 1859 2289
Size: 1643 216 430
Fragments arranged by size:
1643 430 216
NgoMIV G'CCGG_C
Cuts at: 0 847 2289
Size: 847 1442
NspI r_CATG'y
Cuts at: 0 456 2062 2289
Size: 456 1606 227
Fragments arranged by size:
1606 456 227
PasI CC'CwG_GG
Cuts at: 0 1545 2289
Size: 1545 744
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 880 2289
Size: 234 646 1409
Fragments arranged by size:
1409 646 234
PmlI CAC'GTG
Cuts at: 0 726 2289
Size: 726 1563
PpuMI rG'GwC_Cy
Cuts at: 0 893 2289
Size: 893 1396
PspOMI G'GGCC_C
Cuts at: 0 1961 2289
Size: 1961 328
PstI C_TGCA'G
Cuts at: 0 324 1679 1688 2289
Size: 324 1355 9 601
Fragments arranged by size:
1355 601 324 9
PvuII CAG'CTG
Cuts at: 0 174 306 828 1674 1877 1893 2038 2289
Size: 174 132 522 846 203 16 145 251
Fragments arranged by size:
846 522 251 203 174 145 132 16
SexAI A'CCwGG_T
Cuts at: 0 620 673 2289
Size: 620 53 1616
Fragments arranged by size:
1616 620 53
SfcI C'TryA_G
Cuts at: 0 320 940 1675 1684 2289
Size: 320 620 735 9 605
Fragments arranged by size:
735 620 605 320 9
SmaI CCC'GGG
Cuts at: 0 364 923 1801 2289
Size: 364 559 878 488
Fragments arranged by size:
878 559 488 364
SpeI A'CTAG_T
Cuts at: 0 1074 2289
Size: 1074 1215
StuI AGG'CCT
Cuts at: 0 1102 2289
Size: 1102 1187
StyI C'CwwG_G
Cuts at: 0 56 148 314 954 1039 1062 1103 1643
Size: 56 92 166 640 85 23 41 540
Cuts at: 1643 1859 1957 2289
Size: 216 98 332
Fragments arranged by size:
640 540 332 216 166 98 92 85
56 41 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1576 2019 2289
Size: 1576 443 270
Fragments arranged by size:
1576 443 270
TatI w'GTAC_w
Cuts at: 0 1178 2289
Size: 1178 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 971 1003 2289
Size: 971 32 1286
Fragments arranged by size:
1286 971 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 2289
Size: 54 2235
XmaI C'CCGG_G
Cuts at: 0 362 921 1799 2289
Size: 362 559 878 490
Fragments arranged by size:
878 559 490 362
XmnI GAAnn'nnTTC
Cuts at: 0 790 2289
Size: 790 1499
Enzymes that do cut:
AarI Acc65I AcuI AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
BtgI BtsI CspCI DraIII DrdI EaeI Eco57MI EcoNI
EcoO109I FseI HaeII Hin4I HincII KpnI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PmlI PpuMI PspOMI PstI PvuII SexAI SfcI SmaI
SpeI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BclI BfrBI BmtI BplI BpuEI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BstBI BstZ17I Bsu36I BtgZI ClaI DraI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FspI FspAI
HindIII HpaI KasI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmlI SnaBI SphI SrfI SspI SwaI Tth111I
XbaI XhoI ZraI