Restriction map for
pFN21AE5783
(Linear) (Six-base) MAPSORT of: KIEE5783.seq Check: 985 from: 1 to: 2043
KIEE5783.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1221 2043
Size: 1221 822
AatII G_ACGT'C
Cuts at: 0 215 2043
Size: 215 1828
AccI GT'mk_AC
Cuts at: 0 1338 2043
Size: 1338 705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 1037 1188 1241 2043
Size: 692 345 151 53 802
Fragments arranged by size:
802 692 345 151 53
AleI CACnn'nnGTG
Cuts at: 0 1518 2043
Size: 1518 525
AlwNI CAG_nnn'CTG
Cuts at: 0 1219 1236 1684 1708 1917 2043
Size: 1219 17 448 24 209 126
Fragments arranged by size:
1219 448 209 126 24 17
ApoI r'AATT_y
Cuts at: 0 424 495 1943 2043
Size: 424 71 1448 100
Fragments arranged by size:
1448 424 100 71
BanII G_rGCy'C
Cuts at: 0 998 2043
Size: 998 1045
BbsI GAAGACnn'nnnn_
Cuts at: 0 698 1345 2043
Size: 698 647 698
Fragments arranged by size:
698 698 647
BbvCI CC'TCA_GC
Cuts at: 0 1301 1574 2043
Size: 1301 273 469
Fragments arranged by size:
1301 469 273
BglI GCCn_nnn'nGGC
Cuts at: 0 58 2043
Size: 58 1985
Bme1580I G_kGCm'C
Cuts at: 0 687 1316 1606 2043
Size: 687 629 290 437
Fragments arranged by size:
687 629 437 290
BmrI ACTGGGnnnn_n'
Cuts at: 0 945 1067 2043
Size: 945 122 976
Fragments arranged by size:
976 945 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1952 2043
Size: 1952 91
Bpu10I CC'TnA_GC
Cuts at: 0 782 1301 1574 2043
Size: 782 519 273 469
Fragments arranged by size:
782 519 469 273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 2043
Size: 521 1522
BsaI GGTCTCn'nnnn_
Cuts at: 0 830 2043
Size: 830 1213
BsaHI Gr'CG_yC
Cuts at: 0 212 232 2043
Size: 212 20 1811
Fragments arranged by size:
1811 212 20
BsaWI w'CCGG_w
Cuts at: 0 12 162 2043
Size: 12 150 1881
Fragments arranged by size:
1881 150 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 24 2043
Size: 24 2019
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 841 1239 1591 1649 2043
Size: 841 398 352 58 394
Fragments arranged by size:
841 398 394 352 58
BseYI C'CCAG_C
Cuts at: 0 46 1773 1816 2043
Size: 46 1727 43 227
Fragments arranged by size:
1727 227 46 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1496 2043
Size: 782 714 547
Fragments arranged by size:
782 714 547
BsiHKAI G_wGCw'C
Cuts at: 0 833 1213 1749 2043
Size: 833 380 536 294
Fragments arranged by size:
833 536 380 294
BsmI GAATG_Cn'
Cuts at: 0 397 783 1158 1913 2043
Size: 397 386 375 755 130
Fragments arranged by size:
755 397 386 375 130
Bsp1286I G_dGCh'C
Cuts at: 0 687 833 998 1213 1316 1606 1749 2043
Size: 687 146 165 215 103 290 143 294
Fragments arranged by size:
687 294 290 215 165 146 143 103
BspEI T'CCGG_A
Cuts at: 0 12 2043
Size: 12 2031
BspHI T'CATG_A
Cuts at: 0 1793 2043
Size: 1793 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1221 2043
Size: 1221 822
BsrDI GCAATG_nn'
Cuts at: 0 285 575 1044 2043
Size: 285 290 469 999
Fragments arranged by size:
999 469 290 285
BssSI C'ACGA_G
Cuts at: 0 686 1742 2043
Size: 686 1056 301
Fragments arranged by size:
1056 686 301
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 351 1153 1311 2043
Size: 150 201 802 158 732
Fragments arranged by size:
802 732 201 158 150
BstBI TT'CG_AA
Cuts at: 0 417 2043
Size: 417 1626
BstEII G'GTnAC_C
Cuts at: 0 1963 2043
Size: 1963 80
BstYI r'GATC_y
Cuts at: 0 114 603 2043
Size: 114 489 1440
Fragments arranged by size:
1440 489 114
BstZ17I GTA'TAC
Cuts at: 0 1339 2043
Size: 1339 704
Bsu36I CC'TnA_GG
Cuts at: 0 359 2043
Size: 359 1684
BtgI C'CryG_G
Cuts at: 0 697 2043
Size: 697 1346
BtsI GCAGTG_nn'
Cuts at: 0 41 1747 2043
Size: 41 1706 296
Fragments arranged by size:
1706 296 41
ClaI AT'CG_AT
Cuts at: 0 1242 2043
Size: 1242 801
DraI TTT'AAA
Cuts at: 0 494 1610 2043
Size: 494 1116 433
Fragments arranged by size:
1116 494 433
DraIII CAC_nnn'GTG
Cuts at: 0 150 2043
Size: 150 1893
DrdI GACnn_nn'nnGTC
Cuts at: 0 1120 2043
Size: 1120 923
EaeI y'GGCC_r
Cuts at: 0 119 1007 2043
Size: 119 888 1036
Fragments arranged by size:
1036 888 119
EarI CTCTTCn'nnn_
Cuts at: 0 499 1207 1795 2043
Size: 499 708 588 248
Fragments arranged by size:
708 588 499 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 1037 1188 1241 1952 2043
Size: 692 345 151 53 711 91
Fragments arranged by size:
711 692 345 151 91 53
EcoO109I rG'GnC_Cy
Cuts at: 0 872 1914 2043
Size: 872 1042 129
Fragments arranged by size:
1042 872 129
EcoRI G'AATT_C
Cuts at: 0 424 2043
Size: 424 1619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 656 931 2043
Size: 656 275 1112
Fragments arranged by size:
1112 656 275
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 1722 1754 2043
Size: 26 1696 32 289
Fragments arranged by size:
1696 289 32 26
MfeI C'AATT_G
Cuts at: 0 1370 2043
Size: 1370 673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 279 1297 2043
Size: 152 127 1018 746
Fragments arranged by size:
1018 746 152 127
MscI TGG'CCA
Cuts at: 0 121 2043
Size: 121 1922
MslI CAynn'nnrTG
Cuts at: 0 1518 1635 2000 2043
Size: 1518 117 365 43
Fragments arranged by size:
1518 365 117 43
MspA1I CmG'CkG
Cuts at: 0 240 1015 1219 1573 1861 2043
Size: 240 775 204 354 288 182
Fragments arranged by size:
775 354 288 240 204 182
NcoI C'CATG_G
Cuts at: 0 697 2043
Size: 697 1346
NspI r_CATG'y
Cuts at: 0 990 2043
Size: 990 1053
PflMI CCAn_nnn'nTGG
Cuts at: 0 1891 2030 2043
Size: 1891 139 13
Fragments arranged by size:
1891 139 13
PfoI T'CCnGG_A
Cuts at: 0 1382 2043
Size: 1382 661
PpuMI rG'GwC_Cy
Cuts at: 0 872 2043
Size: 872 1171
PsiI TTA'TAA
Cuts at: 0 587 2043
Size: 587 1456
PstI C_TGCA'G
Cuts at: 0 1692 1754 2043
Size: 1692 62 289
Fragments arranged by size:
1692 289 62
PvuII CAG'CTG
Cuts at: 0 240 1015 1219 1573 1861 2043
Size: 240 775 204 354 288 182
Fragments arranged by size:
775 354 288 240 204 182
SanDI GG'GwC_CC
Cuts at: 0 872 2043
Size: 872 1171
SapI GCTCTTCn'nnn_
Cuts at: 0 1795 2043
Size: 1795 248
ScaI AGT'ACT
Cuts at: 0 1295 2043
Size: 1295 748
SfcI C'TryA_G
Cuts at: 0 847 1688 1700 1750 1856 2043
Size: 847 841 12 50 106 187
Fragments arranged by size:
847 841 187 106 50 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 58 2043
Size: 58 1985
SmlI C'TyrA_G
Cuts at: 0 536 2043
Size: 536 1507
SphI G_CATG'C
Cuts at: 0 990 2043
Size: 990 1053
SspI AAT'ATT
Cuts at: 0 291 747 2043
Size: 291 456 1296
Fragments arranged by size:
1296 456 291
StyI C'CwwG_G
Cuts at: 0 697 752 962 1959 2043
Size: 697 55 210 997 84
Fragments arranged by size:
997 697 210 84 55
SwaI ATTT'AAAT
Cuts at: 0 494 2043
Size: 494 1549
TatI w'GTAC_w
Cuts at: 0 319 378 1293 2043
Size: 319 59 915 750
Fragments arranged by size:
915 750 319 59
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 2043
Size: 129 1914
XmnI GAAnn'nnTTC
Cuts at: 0 1243 1559 2043
Size: 1243 316 484
Fragments arranged by size:
1243 484 316
ZraI GAC'GTC
Cuts at: 0 213 2043
Size: 213 1830
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlwNI ApoI BanII
BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrDI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtsI ClaI
DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I EcoRI
FalI Hin4I MfeI MmeI MscI MslI MspA1I NcoI
NspI PflMI PfoI PpuMI PsiI PstI PvuII SanDI
SapI ScaI SfcI SfiI SmlI SphI SspI StyI
SwaI TatI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsiEI
BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BstXI BtgZI
CspCI EagI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI TaqII TstI Tth111I XbaI XhoI XmaI