Restriction map for
pFN21AE5782
(Linear) (Six-base) MAPSORT of: KIEE5782.seq Check: 3185 from: 1 to: 2052
KIEE5782.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AccI GT'mk_AC
Cuts at: 0 1622 2052
Size: 1622 430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 569 2052
Size: 337 232 1483
Fragments arranged by size:
1483 337 232
AflII C'TTAA_G
Cuts at: 0 1285 2052
Size: 1285 767
AgeI A'CCGG_T
Cuts at: 0 699 2052
Size: 699 1353
AhdI GACnn_n'nnGTC
Cuts at: 0 1174 2052
Size: 1174 878
ApoI r'AATT_y
Cuts at: 0 1772 2052
Size: 1772 280
AseI AT'TA_AT
Cuts at: 0 301 1024 2052
Size: 301 723 1028
Fragments arranged by size:
1028 723 301
BanII G_rGCy'C
Cuts at: 0 1749 2052
Size: 1749 303
BbvCI CC'TCA_GC
Cuts at: 0 721 2052
Size: 721 1331
BclI T'GATC_A
Cuts at: 0 228 255 2052
Size: 228 27 1797
Fragments arranged by size:
1797 228 27
BfrBI ATG'CAT
Cuts at: 0 876 928 1921 2052
Size: 876 52 993 131
Fragments arranged by size:
993 876 131 52
BglII A'GATC_T
Cuts at: 0 177 1363 2052
Size: 177 1186 689
Fragments arranged by size:
1186 689 177
Bme1580I G_kGCm'C
Cuts at: 0 1280 2052
Size: 1280 772
BmrI ACTGGGnnnn_n'
Cuts at: 0 1635 2052
Size: 1635 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 772 1019 1166 2052
Size: 557 215 247 147 886
Fragments arranged by size:
886 557 247 215 147
Bpu10I CC'TnA_GC
Cuts at: 0 721 2052
Size: 721 1331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1978 2052
Size: 646 1332 74
Fragments arranged by size:
1332 646 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 1342 2052
Size: 1342 710
BsaBI GATnn'nnATC
Cuts at: 0 56 1672 2052
Size: 56 1616 380
Fragments arranged by size:
1616 380 56
BsaWI w'CCGG_w
Cuts at: 0 699 2052
Size: 699 1353
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 269 299 2052
Size: 269 30 1753
Fragments arranged by size:
1753 269 30
BseYI C'CCAG_C
Cuts at: 0 693 811 1439 1983 2052
Size: 693 118 628 544 69
Fragments arranged by size:
693 628 544 118 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 394 2052
Size: 355 39 1658
Fragments arranged by size:
1658 355 39
BsmI GAATG_Cn'
Cuts at: 0 1923 2052
Size: 1923 129
Bsp1286I G_dGCh'C
Cuts at: 0 1280 1749 2052
Size: 1280 469 303
Fragments arranged by size:
1280 469 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1285 2052
Size: 1285 767
BsrDI GCAATG_nn'
Cuts at: 0 605 1571 2052
Size: 605 966 481
Fragments arranged by size:
966 605 481
BsrFI r'CCGG_y
Cuts at: 0 699 2052
Size: 699 1353
BssSI C'ACGA_G
Cuts at: 0 1157 2052
Size: 1157 895
BstEII G'GTnAC_C
Cuts at: 0 1549 2052
Size: 1549 503
BstYI r'GATC_y
Cuts at: 0 177 1363 1498 2052
Size: 177 1186 135 554
Fragments arranged by size:
1186 554 177 135
BtgI C'CryG_G
Cuts at: 0 974 2052
Size: 974 1078
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 437 2052
Size: 437 1615
DraI TTT'AAA
Cuts at: 0 1977 2052
Size: 1977 75
DraIII CAC_nnn'GTG
Cuts at: 0 133 2052
Size: 133 1919
EaeI y'GGCC_r
Cuts at: 0 66 2052
Size: 66 1986
EarI CTCTTCn'nnn_
Cuts at: 0 671 2052
Size: 671 1381
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 760 1099 2052
Size: 760 339 953
Fragments arranged by size:
953 760 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 557 569 772 1019 1166 2052
Size: 337 220 12 203 247 147 886
Fragments arranged by size:
886 337 247 220 203 147 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 581 2052
Size: 581 1471
EcoRV GAT'ATC
Cuts at: 0 1674 2052
Size: 1674 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 256 2052
Size: 256 1796
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1160 1192 1483 1515 1736 1768 2052
Size: 1160 32 291 32 221 32 284
Fragments arranged by size:
1160 291 284 221 32 32 32
HincII GTy'rAC
Cuts at: 0 104 1623 2052
Size: 104 1519 429
Fragments arranged by size:
1519 429 104
HindIII A'AGCT_T
Cuts at: 0 911 1727 2052
Size: 911 816 325
Fragments arranged by size:
911 816 325
MslI CAynn'nnrTG
Cuts at: 0 14 806 881 927 2052
Size: 14 792 75 46 1125
Fragments arranged by size:
1125 792 75 46 14
MspA1I CmG'CkG
Cuts at: 0 109 2052
Size: 109 1943
NcoI C'CATG_G
Cuts at: 0 974 2052
Size: 974 1078
NdeI CA'TA_TG
Cuts at: 0 836 924 2052
Size: 836 88 1128
Fragments arranged by size:
1128 836 88
NruI TCG'CGA
Cuts at: 0 456 2052
Size: 456 1596
NsiI A_TGCA'T
Cuts at: 0 878 930 1923 2052
Size: 878 52 993 129
Fragments arranged by size:
993 878 129 52
PvuII CAG'CTG
Cuts at: 0 109 2052
Size: 109 1943
SalI G'TCGA_C
Cuts at: 0 1621 2052
Size: 1621 431
SapI GCTCTTCn'nnn_
Cuts at: 0 671 2052
Size: 671 1381
ScaI AGT'ACT
Cuts at: 0 822 2052
Size: 822 1230
SfcI C'TryA_G
Cuts at: 0 74 410 1933 2052
Size: 74 336 1523 119
Fragments arranged by size:
1523 336 119 74
SgrAI Cr'CCGG_yG
Cuts at: 0 699 2052
Size: 699 1353
SmlI C'TyrA_G
Cuts at: 0 625 1285 1957 2052
Size: 625 660 672 95
Fragments arranged by size:
672 660 625 95
SspI AAT'ATT
Cuts at: 0 299 1767 2052
Size: 299 1468 285
Fragments arranged by size:
1468 299 285
StyI C'CwwG_G
Cuts at: 0 974 2052
Size: 974 1078
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1568 2052
Size: 1568 484
TatI w'GTAC_w
Cuts at: 0 820 2052
Size: 820 1232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 515 547 1781 1813 2052
Size: 269 32 214 32 1234 32 239
Fragments arranged by size:
1234 269 239 214 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 217 2052
Size: 217 1835
XmnI GAAnn'nnTTC
Cuts at: 0 311 1250 2052
Size: 311 939 802
Fragments arranged by size:
939 802 311
Enzymes that do cut:
AccI AcuI AflII AgeI AhdI ApoI AseI BanII
BbvCI BclI BfrBI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BsaXI BseYI BsgI BsmI
Bsp1286I BspMI BsrDI BsrFI BssSI BstEII BstYI BtgI
BtgZI DraI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoRV FalI Hin4I HincII HindIII MslI MspA1I NcoI
NdeI NruI NsiI PvuII SalI SapI ScaI SfcI
SgrAI SmlI SspI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BcgI BciVI BglI
BlpI BmgBI BmtI BplI BsaAI BsaHI BseRI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DrdI EagI EcoICRI EcoO109I EcoRI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NgoMIV NheI NotI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SanDI SbfI SexAI SfiI SfoI SgfI SmaI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI
XhoI XmaI ZraI