Restriction map for
pFN21AE5781
(Linear) (Six-base) MAPSORT of: KIEE5781.seq Check: 3569 from: 1 to: 1932
KIEE5781.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AccI GT'mk_AC
Cuts at: 0 983 1262 1932
Size: 983 279 670
Fragments arranged by size:
983 670 279
Acc65I G'GTAC_C
Cuts at: 0 110 1932
Size: 110 1822
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1932
Size: 326 1606
AfeI AGC'GCT
Cuts at: 0 913 1932
Size: 913 1019
AleI CACnn'nnGTG
Cuts at: 0 149 1873 1932
Size: 149 1724 59
Fragments arranged by size:
1724 149 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1891 1932
Size: 1891 41
AlwNI CAG_nnn'CTG
Cuts at: 0 242 1367 1932
Size: 242 1125 565
Fragments arranged by size:
1125 565 242
ApoI r'AATT_y
Cuts at: 0 364 416 562 854 1932
Size: 364 52 146 292 1078
Fragments arranged by size:
1078 364 292 146 52
AvaI C'yCGr_G
Cuts at: 0 106 1696 1932
Size: 106 1590 236
Fragments arranged by size:
1590 236 106
BanI G'GyrC_C
Cuts at: 0 110 1027 1867 1932
Size: 110 917 840 65
Fragments arranged by size:
917 840 110 65
BanII G_rGCy'C
Cuts at: 0 1727 1932
Size: 1727 205
BbeI G_GCGC'C
Cuts at: 0 1031 1932
Size: 1031 901
BbsI GAAGACnn'nnnn_
Cuts at: 0 258 332 1106 1932
Size: 258 74 774 826
Fragments arranged by size:
826 774 258 74
BciVI GTATCCnnnnn_n'
Cuts at: 0 968 1932
Size: 968 964
BclI T'GATC_A
Cuts at: 0 203 1583 1932
Size: 203 1380 349
Fragments arranged by size:
1380 349 203
Bme1580I G_kGCm'C
Cuts at: 0 1416 1932
Size: 1416 516
BmrI ACTGGGnnnn_n'
Cuts at: 0 879 1729 1932
Size: 879 850 203
Fragments arranged by size:
879 850 203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 606 1724 1932
Size: 606 1118 208
Fragments arranged by size:
1118 606 208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 601 1932
Size: 584 17 1331
Fragments arranged by size:
1331 584 17
Bpu10I CC'TnA_GC
Cuts at: 0 1646 1932
Size: 1646 286
BsaAI yAC'GTr
Cuts at: 0 258 1932
Size: 258 1674
BsaBI GATnn'nnATC
Cuts at: 0 714 1932
Size: 714 1218
BsaHI Gr'CG_yC
Cuts at: 0 1028 1932
Size: 1028 904
BsaWI w'CCGG_w
Cuts at: 0 180 1932
Size: 180 1752
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1072 1102 1932
Size: 1072 30 830
Fragments arranged by size:
1072 830 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 317 1486 1932
Size: 10 307 1169 446
Fragments arranged by size:
1169 446 307 10
BseYI C'CCAG_C
Cuts at: 0 1409 1740 1932
Size: 1409 331 192
Fragments arranged by size:
1409 331 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1932
Size: 413 1519
BsiEI CG_ry'CG
Cuts at: 0 56 627 1932
Size: 56 571 1305
Fragments arranged by size:
1305 571 56
BsiHKAI G_wGCw'C
Cuts at: 0 35 1932
Size: 35 1897
BsmI GAATG_Cn'
Cuts at: 0 520 722 1932
Size: 520 202 1210
Fragments arranged by size:
1210 520 202
Bsp1286I G_dGCh'C
Cuts at: 0 35 1416 1727 1932
Size: 35 1381 311 205
Fragments arranged by size:
1381 311 205 35
BspEI T'CCGG_A
Cuts at: 0 180 1932
Size: 180 1752
BspHI T'CATG_A
Cuts at: 0 1532 1932
Size: 1532 400
BsrBI CCG'CTC
Cuts at: 0 18 1932
Size: 18 1914
BsrFI r'CCGG_y
Cuts at: 0 55 626 1932
Size: 55 571 1306
Fragments arranged by size:
1306 571 55
BstEII G'GTnAC_C
Cuts at: 0 1392 1932
Size: 1392 540
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1878 1932
Size: 1878 54
BstYI r'GATC_y
Cuts at: 0 1688 1932
Size: 1688 244
BstZ17I GTA'TAC
Cuts at: 0 1263 1932
Size: 1263 669
BtgI C'CryG_G
Cuts at: 0 731 1895 1932
Size: 731 1164 37
Fragments arranged by size:
1164 731 37
BtsI GCAGTG_nn'
Cuts at: 0 807 1932
Size: 807 1125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1149 1184 1932
Size: 1149 35 748
Fragments arranged by size:
1149 748 35
DraIII CAC_nnn'GTG
Cuts at: 0 391 463 1932
Size: 391 72 1469
Fragments arranged by size:
1469 391 72
EaeI y'GGCC_r
Cuts at: 0 53 1880 1932
Size: 53 1827 52
Fragments arranged by size:
1827 53 52
EagI C'GGCC_G
Cuts at: 0 53 1932
Size: 53 1879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1375 1730 1932
Size: 1375 355 202
Fragments arranged by size:
1375 355 202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 584 601 1932
Size: 326 258 17 1331
Fragments arranged by size:
1331 326 258 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1468 1932
Size: 1468 464
EcoO109I rG'GnC_Cy
Cuts at: 0 430 1932
Size: 430 1502
HaeII r_GCGC'y
Cuts at: 0 62 915 1031 1932
Size: 62 853 116 901
Fragments arranged by size:
901 853 116 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 162 194 309 341 600 632 1072 1104
Size: 162 32 115 32 259 32 440 32
Cuts at: 1104 1709 1741 1932
Size: 605 32 191
Fragments arranged by size:
605 440 259 191 162 115 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 570 1932
Size: 570 1362
KasI G'GCGC_C
Cuts at: 0 1027 1932
Size: 1027 905
KpnI G_GTAC'C
Cuts at: 0 114 1932
Size: 114 1818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1373 1932
Size: 1115 258 559
Fragments arranged by size:
1115 559 258
MscI TGG'CCA
Cuts at: 0 1882 1932
Size: 1882 50
MslI CAynn'nnrTG
Cuts at: 0 149 1246 1873 1932
Size: 149 1097 627 59
Fragments arranged by size:
1097 627 149 59
MspA1I CmG'CkG
Cuts at: 0 1768 1932
Size: 1768 164
NaeI GCC'GGC
Cuts at: 0 57 1932
Size: 57 1875
NarI GG'CG_CC
Cuts at: 0 1028 1932
Size: 1028 904
NcoI C'CATG_G
Cuts at: 0 731 1932
Size: 731 1201
NgoMIV G'CCGG_C
Cuts at: 0 55 1932
Size: 55 1877
PfoI T'CCnGG_A
Cuts at: 0 557 948 1932
Size: 557 391 984
Fragments arranged by size:
984 557 391
PpuMI rG'GwC_Cy
Cuts at: 0 430 1932
Size: 430 1502
PstI C_TGCA'G
Cuts at: 0 909 1932
Size: 909 1023
PvuII CAG'CTG
Cuts at: 0 1768 1932
Size: 1768 164
SexAI A'CCwGG_T
Cuts at: 0 696 1932
Size: 696 1236
SfcI C'TryA_G
Cuts at: 0 783 905 1763 1932
Size: 783 122 858 169
Fragments arranged by size:
858 783 169 122
SfoI GGC'GCC
Cuts at: 0 1029 1932
Size: 1029 903
SmaI CCC'GGG
Cuts at: 0 108 1932
Size: 108 1824
SspI AAT'ATT
Cuts at: 0 1057 1932
Size: 1057 875
StyI C'CwwG_G
Cuts at: 0 731 1353 1932
Size: 731 622 579
Fragments arranged by size:
731 622 579
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 161 929 1788 1932
Size: 161 768 859 144
Fragments arranged by size:
859 768 161 144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1167 1791 1861 1932
Size: 1167 624 70 71
Fragments arranged by size:
1167 624 71 70
XmaI C'CCGG_G
Cuts at: 0 106 1932
Size: 106 1826
Enzymes that do cut:
AccI Acc65I AcuI AfeI AleI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BciVI BclI Bme1580I
BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BsrBI BsrFI BstEII BstXI BstYI BstZ17I
BtgI BtsI CspCI DraIII EaeI EagI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PfoI
PpuMI PstI PvuII SexAI SfcI SfoI SmaI SspI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BfrBI BglI BglII BlpI BmgBI BmtI
BpuEI BsaI BsiWI BsmBI BspMI BsrDI BsrGI BssHII
BssSI BstAPI BstBI Bsu36I BtgZI ClaI DraI DrdI
EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI MfeI MluI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI