Restriction map for
pFN21AE5776
(Linear) (Six-base) MAPSORT of: KIEE5776.seq Check: 9934 from: 1 to: 1872
KIEE5776.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1826 1872
Size: 329 1497 46
Fragments arranged by size:
1497 329 46
AfeI AGC'GCT
Cuts at: 0 1051 1872
Size: 1051 821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1285 1433 1872
Size: 1285 148 439
Fragments arranged by size:
1285 439 148
AlwNI CAG_nnn'CTG
Cuts at: 0 367 1872
Size: 367 1505
ApaLI G'TGCA_C
Cuts at: 0 1270 1872
Size: 1270 602
ApoI r'AATT_y
Cuts at: 0 431 1216 1750 1872
Size: 431 785 534 122
Fragments arranged by size:
785 534 431 122
AseI AT'TA_AT
Cuts at: 0 776 1872
Size: 776 1096
AvaI C'yCGr_G
Cuts at: 0 10 530 1784 1872
Size: 10 520 1254 88
Fragments arranged by size:
1254 520 88 10
BanI G'GyrC_C
Cuts at: 0 1334 1624 1872
Size: 1334 290 248
Fragments arranged by size:
1334 290 248
BbeI G_GCGC'C
Cuts at: 0 1338 1872
Size: 1338 534
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 877 1748 1872
Size: 317 560 871 124
Fragments arranged by size:
871 560 317 124
BciVI GTATCCnnnnn_n'
Cuts at: 0 1293 1872
Size: 1293 579
BclI T'GATC_A
Cuts at: 0 1179 1872
Size: 1179 693
BfrBI ATG'CAT
Cuts at: 0 688 1872
Size: 688 1184
BglII A'GATC_T
Cuts at: 0 1137 1872
Size: 1137 735
BlpI GC'TnA_GC
Cuts at: 0 37 1872
Size: 37 1835
Bme1580I G_kGCm'C
Cuts at: 0 1274 1872
Size: 1274 598
BmrI ACTGGGnnnn_n'
Cuts at: 0 66 566 1261 1872
Size: 66 500 695 611
Fragments arranged by size:
695 611 500 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1872
Size: 203 1669
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 1559 1872
Size: 286 1273 313
Fragments arranged by size:
1273 313 286
BsaBI GATnn'nnATC
Cuts at: 0 1184 1872
Size: 1184 688
BsaHI Gr'CG_yC
Cuts at: 0 1335 1872
Size: 1335 537
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 470 1060 1148 1336 1339 1872
Size: 232 238 590 88 188 3 533
Fragments arranged by size:
590 533 238 232 188 88 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1872
Size: 820 1052
BsiHKAI G_wGCw'C
Cuts at: 0 1037 1274 1527 1872
Size: 1037 237 253 345
Fragments arranged by size:
1037 345 253 237
BsmI GAATG_Cn'
Cuts at: 0 68 1236 1775 1872
Size: 68 1168 539 97
Fragments arranged by size:
1168 539 97 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1444 1872
Size: 1444 428
Bsp1286I G_dGCh'C
Cuts at: 0 1037 1274 1527 1872
Size: 1037 237 253 345
Fragments arranged by size:
1037 345 253 237
BspHI T'CATG_A
Cuts at: 0 435 939 1872
Size: 435 504 933
Fragments arranged by size:
933 504 435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 792 1872
Size: 792 1080
BsrBI CCG'CTC
Cuts at: 0 187 1872
Size: 187 1685
BsrDI GCAATG_nn'
Cuts at: 0 1515 1872
Size: 1515 357
BsrFI r'CCGG_y
Cuts at: 0 1331 1872
Size: 1331 541
BsrGI T'GTAC_A
Cuts at: 0 967 1872
Size: 967 905
BstAPI GCAn_nnn'nTGC
Cuts at: 0 346 798 1872
Size: 346 452 1074
Fragments arranged by size:
1074 452 346
BstYI r'GATC_y
Cuts at: 0 5 1137 1602 1872
Size: 5 1132 465 270
Fragments arranged by size:
1132 465 270 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 321 1872
Size: 321 1551
BtsI GCAGTG_nn'
Cuts at: 0 799 871 1427 1872
Size: 799 72 556 445
Fragments arranged by size:
799 556 445 72
ClaI AT'CG_AT
Cuts at: 0 563 1872
Size: 563 1309
DraI TTT'AAA
Cuts at: 0 1221 1872
Size: 1221 651
EaeI y'GGCC_r
Cuts at: 0 1004 1463 1872
Size: 1004 459 409
Fragments arranged by size:
1004 459 409
EarI CTCTTCn'nnn_
Cuts at: 0 314 1872
Size: 314 1558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 329 1826 1872
Size: 203 126 1497 46
Fragments arranged by size:
1497 203 126 46
EcoO109I rG'GnC_Cy
Cuts at: 0 588 1872
Size: 588 1284
FspI TGC'GCA
Cuts at: 0 1774 1872
Size: 1774 98
HaeII r_GCGC'y
Cuts at: 0 1053 1338 1872
Size: 1053 285 534
Fragments arranged by size:
1053 534 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 921 953 1872
Size: 921 32 919
Fragments arranged by size:
921 919 32
KasI G'GCGC_C
Cuts at: 0 1334 1872
Size: 1334 538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1406 1539 1872
Size: 1406 133 333
Fragments arranged by size:
1406 333 133
MscI TGG'CCA
Cuts at: 0 1006 1465 1872
Size: 1006 459 407
Fragments arranged by size:
1006 459 407
MslI CAynn'nnrTG
Cuts at: 0 440 491 1872
Size: 440 51 1381
Fragments arranged by size:
1381 440 51
NarI GG'CG_CC
Cuts at: 0 1335 1872
Size: 1335 537
NsiI A_TGCA'T
Cuts at: 0 690 1872
Size: 690 1182
NspI r_CATG'y
Cuts at: 0 490 1079 1285 1417 1581 1872
Size: 490 589 206 132 164 291
Fragments arranged by size:
589 490 291 206 164 132
PflMI CCAn_nnn'nTGG
Cuts at: 0 835 1064 1359 1621 1829 1872
Size: 835 229 295 262 208 43
Fragments arranged by size:
835 295 262 229 208 43
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 908 940 1872
Size: 908 32 932
Fragments arranged by size:
932 908 32
PstI C_TGCA'G
Cuts at: 0 1422 1872
Size: 1422 450
ScaI AGT'ACT
Cuts at: 0 859 1872
Size: 859 1013
SexAI A'CCwGG_T
Cuts at: 0 224 1872
Size: 224 1648
SfcI C'TryA_G
Cuts at: 0 658 920 1418 1872
Size: 658 262 498 454
Fragments arranged by size:
658 498 454 262
SfoI GGC'GCC
Cuts at: 0 1336 1872
Size: 1336 536
SmaI CCC'GGG
Cuts at: 0 12 532 1872
Size: 12 520 1340
Fragments arranged by size:
1340 520 12
SmlI C'TyrA_G
Cuts at: 0 1784 1872
Size: 1784 88
SphI G_CATG'C
Cuts at: 0 1417 1581 1872
Size: 1417 164 291
Fragments arranged by size:
1417 291 164
SrfI GCCC'GGGC
Cuts at: 0 532 1872
Size: 532 1340
SspI AAT'ATT
Cuts at: 0 270 1872
Size: 270 1602
StyI C'CwwG_G
Cuts at: 0 212 1823 1859 1872
Size: 212 1611 36 13
Fragments arranged by size:
1611 212 36 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 786 1351 1872
Size: 786 565 521
Fragments arranged by size:
786 565 521
TatI w'GTAC_w
Cuts at: 0 857 967 1872
Size: 857 110 905
Fragments arranged by size:
905 857 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 126 158 778 810 1872
Size: 126 32 620 32 1062
Fragments arranged by size:
1062 620 126 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 634 1872
Size: 634 1238
XbaI T'CTAG_A
Cuts at: 0 1317 1872
Size: 1317 555
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1360 1872
Size: 1360 512
XhoI C'TCGA_G
Cuts at: 0 1784 1872
Size: 1784 88
XmaI C'CCGG_G
Cuts at: 0 10 530 1872
Size: 10 520 1342
Fragments arranged by size:
1342 520 10
XmnI GAAnn'nnTTC
Cuts at: 0 1436 1670 1872
Size: 1436 234 202
Fragments arranged by size:
1436 234 202
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaLI ApoI AseI AvaI
BanI BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BpmI BsaI BsaBI BsaHI BseRI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstYI BtgZI BtsI ClaI DraI
EaeI EarI Eco57MI EcoO109I FspI HaeII Hin4I KasI
MmeI MscI MslI NarI NsiI NspI PflMI PsrI
PstI ScaI SexAI SfcI SfoI SmaI SmlI SphI
SrfI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AsiSI AvrII BaeI
BamHI BanII BbvCI BcgI BglI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaWI BsaXI BseYI BsiEI BsiWI
BspEI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SfiI SgfI SgrAI SnaBI SpeI StuI SwaI ZraI