Restriction map for
pFN21AE5774
(Linear) (Six-base) MAPSORT of: KIEE5774.seq Check: 5393 from: 1 to: 1866
KIEE5774.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1426 1584 1866
Size: 1076 350 158 282
Fragments arranged by size:
1076 350 282 158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 728 1866
Size: 728 1138
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 280 312 1866
Size: 280 32 1554
Fragments arranged by size:
1554 280 32
ApoI r'AATT_y
Cuts at: 0 1194 1523 1800 1819 1866
Size: 1194 329 277 19 47
Fragments arranged by size:
1194 329 277 47 19
BanI G'GyrC_C
Cuts at: 0 117 1866
Size: 117 1749
BanII G_rGCy'C
Cuts at: 0 441 770 1640 1782 1866
Size: 441 329 870 142 84
Fragments arranged by size:
870 441 329 142 84
BbeI G_GCGC'C
Cuts at: 0 121 1866
Size: 121 1745
BbsI GAAGACnn'nnnn_
Cuts at: 0 979 1442 1866
Size: 979 463 424
Fragments arranged by size:
979 463 424
BbvCI CC'TCA_GC
Cuts at: 0 386 680 1866
Size: 386 294 1186
Fragments arranged by size:
1186 386 294
BciVI GTATCCnnnnn_n'
Cuts at: 0 875 1866
Size: 875 991
BmgBI CAC'GTC
Cuts at: 0 723 1866
Size: 723 1143
BmrI ACTGGGnnnn_n'
Cuts at: 0 436 1460 1636 1866
Size: 436 1024 176 230
Fragments arranged by size:
1024 436 230 176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 321 330 587 1132 1866
Size: 30 291 9 257 545 734
Fragments arranged by size:
734 545 291 257 30 9
Bpu10I CC'TnA_GC
Cuts at: 0 386 680 1866
Size: 386 294 1186
Fragments arranged by size:
1186 386 294
BsaI GGTCTCn'nnnn_
Cuts at: 0 940 1300 1866
Size: 940 360 566
Fragments arranged by size:
940 566 360
BsaHI Gr'CG_yC
Cuts at: 0 118 1866
Size: 118 1748
BsaWI w'CCGG_w
Cuts at: 0 750 1866
Size: 750 1116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 428 458 1866
Size: 280 30 118 30 1408
Fragments arranged by size:
1408 280 118 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 449 1648 1866
Size: 449 1199 218
Fragments arranged by size:
1199 449 218
BseYI C'CCAG_C
Cuts at: 0 1168 1866
Size: 1168 698
BsiHKAI G_wGCw'C
Cuts at: 0 1640 1850 1866
Size: 1640 210 16
Fragments arranged by size:
1640 210 16
Bsp1286I G_dGCh'C
Cuts at: 0 441 770 1640 1782 1850 1866
Size: 441 329 870 142 68 16
Fragments arranged by size:
870 441 329 142 68 16
BspEI T'CCGG_A
Cuts at: 0 750 1866
Size: 750 1116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1600 1866
Size: 1600 266
BstXI CCAn_nnnn'nTGG
Cuts at: 0 180 1866
Size: 180 1686
BstYI r'GATC_y
Cuts at: 0 1442 1866
Size: 1442 424
Bsu36I CC'TnA_GG
Cuts at: 0 616 1866
Size: 616 1250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 288 1866
Size: 288 1578
DraI TTT'AAA
Cuts at: 0 137 1866
Size: 137 1729
EaeI y'GGCC_r
Cuts at: 0 608 1154 1866
Size: 608 546 712
Fragments arranged by size:
712 608 546
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 406 1649 1866
Size: 406 1243 217
Fragments arranged by size:
1243 406 217
EcoICRI GAG'CTC
Cuts at: 0 1638 1866
Size: 1638 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 321 330 587 1076 1132 1426 1584
Size: 30 291 9 257 489 56 294 158
Cuts at: 1584 1866
Size: 282
Fragments arranged by size:
489 294 291 282 257 158 56 30
9
EcoO109I rG'GnC_Cy
Cuts at: 0 940 1703 1866
Size: 940 763 163
Fragments arranged by size:
940 763 163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1744 1866
Size: 1744 122
HaeII r_GCGC'y
Cuts at: 0 121 1866
Size: 121 1745
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 266 298 428 460 745 777 1866
Size: 266 32 130 32 285 32 1089
Fragments arranged by size:
1089 285 266 130 32 32 32
HincII GTy'rAC
Cuts at: 0 165 189 1866
Size: 165 24 1677
Fragments arranged by size:
1677 165 24
KasI G'GCGC_C
Cuts at: 0 117 1866
Size: 117 1749
MscI TGG'CCA
Cuts at: 0 610 1156 1866
Size: 610 546 710
Fragments arranged by size:
710 610 546
MslI CAynn'nnrTG
Cuts at: 0 648 733 859 967 1379 1766 1866
Size: 648 85 126 108 412 387 100
Fragments arranged by size:
648 412 387 126 108 100 85
MspA1I CmG'CkG
Cuts at: 0 176 385 1866
Size: 176 209 1481
Fragments arranged by size:
1481 209 176
NarI GG'CG_CC
Cuts at: 0 118 1866
Size: 118 1748
NdeI CA'TA_TG
Cuts at: 0 472 1290 1866
Size: 472 818 576
Fragments arranged by size:
818 576 472
PasI CC'CwG_GG
Cuts at: 0 1617 1866
Size: 1617 249
PflMI CCAn_nnn'nTGG
Cuts at: 0 693 809 1866
Size: 693 116 1057
Fragments arranged by size:
1057 693 116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 1866
Size: 280 32 1554
Fragments arranged by size:
1554 280 32
PpuMI rG'GwC_Cy
Cuts at: 0 940 1703 1866
Size: 940 763 163
Fragments arranged by size:
940 763 163
PsiI TTA'TAA
Cuts at: 0 584 1866
Size: 584 1282
PstI C_TGCA'G
Cuts at: 0 245 1866
Size: 245 1621
PvuII CAG'CTG
Cuts at: 0 176 385 1866
Size: 176 209 1481
Fragments arranged by size:
1481 209 176
SacI G_AGCT'C
Cuts at: 0 1640 1866
Size: 1640 226
SanDI GG'GwC_CC
Cuts at: 0 1703 1866
Size: 1703 163
SfcI C'TryA_G
Cuts at: 0 241 1866
Size: 241 1625
SfoI GGC'GCC
Cuts at: 0 119 1866
Size: 119 1747
StuI AGG'CCT
Cuts at: 0 670 1259 1605 1866
Size: 670 589 346 261
Fragments arranged by size:
670 589 346 261
StyI C'CwwG_G
Cuts at: 0 950 1853 1866
Size: 950 903 13
Fragments arranged by size:
950 903 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 555 1866
Size: 555 1311
Tth111I GACn'n_nGTC
Cuts at: 0 1206 1866
Size: 1206 660
XbaI T'CTAG_A
Cuts at: 0 717 1866
Size: 717 1149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1057 1473 1866
Size: 1057 416 393
Fragments arranged by size:
1057 416 393
XmnI GAAnn'nnTTC
Cuts at: 0 746 926 1838 1866
Size: 746 180 912 28
Fragments arranged by size:
912 746 180 28
Enzymes that do cut:
AcuI AlfI AloI ApoI BanI BanII BbeI BbsI
BbvCI BciVI BmgBI BmrI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI
BstXI BstYI Bsu36I BtgZI DraI EaeI EciI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MscI
MslI MspA1I NarI NdeI PasI PflMI PpiI PpuMI
PsiI PstI PvuII SacI SanDI SfcI SfoI StuI
StyI TaqII Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BclI BfrBI
BglI BglII BlpI Bme1580I BmtI BplI BpuEI BsaAI
BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtsI ClaI CspCI DraIII DrdI EagI
EarI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI XhoI XmaI ZraI