Restriction map for
pFN21AE5770
(Linear) (Six-base) MAPSORT of: KIEE5770.seq Check: 3771 from: 1 to: 1800
KIEE5770.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 45 1800
Size: 45 1755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 1025 1730 1800
Size: 804 221 705 70
Fragments arranged by size:
804 705 221 70
AfeI AGC'GCT
Cuts at: 0 1101 1800
Size: 1101 699
AflII C'TTAA_G
Cuts at: 0 1690 1800
Size: 1690 110
AflIII A'CryG_T
Cuts at: 0 440 1800
Size: 440 1360
AleI CACnn'nnGTG
Cuts at: 0 976 1800
Size: 976 824
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 427 459 1800
Size: 427 32 1341
Fragments arranged by size:
1341 427 32
AlwNI CAG_nnn'CTG
Cuts at: 0 60 827 1800
Size: 60 767 973
Fragments arranged by size:
973 767 60
ApaLI G'TGCA_C
Cuts at: 0 969 1800
Size: 969 831
ApoI r'AATT_y
Cuts at: 0 206 760 1540 1717 1800
Size: 206 554 780 177 83
Fragments arranged by size:
780 554 206 177 83
AvaI C'yCGr_G
Cuts at: 0 272 328 986 1355 1800
Size: 272 56 658 369 445
Fragments arranged by size:
658 445 369 272 56
BanII G_rGCy'C
Cuts at: 0 22 1800
Size: 22 1778
BbsI GAAGACnn'nnnn_
Cuts at: 0 715 807 1800
Size: 715 92 993
Fragments arranged by size:
993 715 92
BbvCI CC'TCA_GC
Cuts at: 0 1372 1800
Size: 1372 428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 162 196 1800
Size: 162 34 1604
Fragments arranged by size:
1604 162 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1094 1800
Size: 1094 706
BglII A'GATC_T
Cuts at: 0 926 1800
Size: 926 874
Bme1580I G_kGCm'C
Cuts at: 0 705 973 1427 1800
Size: 705 268 454 373
Fragments arranged by size:
705 454 373 268
BmrI ACTGGGnnnn_n'
Cuts at: 0 1023 1800
Size: 1023 777
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 664 1800
Size: 664 1136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 407 586 1516 1800
Size: 389 18 179 930 284
Fragments arranged by size:
930 389 284 179 18
Bpu10I CC'TnA_GC
Cuts at: 0 1372 1800
Size: 1372 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1204 1800
Size: 1204 596
BsaI GGTCTCn'nnnn_
Cuts at: 0 672 1800
Size: 672 1128
BsaHI Gr'CG_yC
Cuts at: 0 97 1800
Size: 97 1703
BsaWI w'CCGG_w
Cuts at: 0 446 650 1800
Size: 446 204 1150
Fragments arranged by size:
1150 446 204
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 362 392 427 457 1800
Size: 362 30 35 30 1343
Fragments arranged by size:
1343 362 35 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 600 1800
Size: 600 1200
BseYI C'CCAG_C
Cuts at: 0 60 78 1036 1800
Size: 60 18 958 764
Fragments arranged by size:
958 764 60 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 124 1800
Size: 31 93 1676
Fragments arranged by size:
1676 93 31
BsiEI CG_ry'CG
Cuts at: 0 1071 1800
Size: 1071 729
BsiHKAI G_wGCw'C
Cuts at: 0 973 1800
Size: 973 827
BsmI GAATG_Cn'
Cuts at: 0 893 1312 1800
Size: 893 419 488
Fragments arranged by size:
893 488 419
Bsp1286I G_dGCh'C
Cuts at: 0 22 705 973 1427 1800
Size: 22 683 268 454 373
Fragments arranged by size:
683 454 373 268 22
BspEI T'CCGG_A
Cuts at: 0 446 650 1800
Size: 446 204 1150
Fragments arranged by size:
1150 446 204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 45 1800
Size: 45 1755
BsrBI CCG'CTC
Cuts at: 0 643 1800
Size: 643 1157
BsrDI GCAATG_nn'
Cuts at: 0 1047 1800
Size: 1047 753
BsrFI r'CCGG_y
Cuts at: 0 99 1800
Size: 99 1701
BssHII G'CGCG_C
Cuts at: 0 65 1800
Size: 65 1735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1233 1800
Size: 1233 567
BstYI r'GATC_y
Cuts at: 0 468 926 1332 1775 1800
Size: 468 458 406 443 25
Fragments arranged by size:
468 458 443 406 25
BtgI C'CryG_G
Cuts at: 0 1089 1121 1226 1427 1800
Size: 1089 32 105 201 373
Fragments arranged by size:
1089 373 201 105 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1045 1800
Size: 1045 755
BtsI GCAGTG_nn'
Cuts at: 0 705 1800
Size: 705 1095
DraI TTT'AAA
Cuts at: 0 1458 1800
Size: 1458 342
EaeI y'GGCC_r
Cuts at: 0 281 416 473 1086 1800
Size: 281 135 57 613 714
Fragments arranged by size:
714 613 281 135 57
EarI CTCTTCn'nnn_
Cuts at: 0 213 218 1121 1503 1616 1800
Size: 213 5 903 382 113 184
Fragments arranged by size:
903 382 213 184 113 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 407 586 804 1025 1516 1730 1800
Size: 389 18 179 218 221 491 214 70
Fragments arranged by size:
491 389 221 218 214 179 70 18
EcoO109I rG'GnC_Cy
Cuts at: 0 300 1800
Size: 300 1500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1678 1800
Size: 1678 122
HaeII r_GCGC'y
Cuts at: 0 1103 1800
Size: 1103 697
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 242 274 289 321 1800
Size: 242 32 15 32 1479
Fragments arranged by size:
1479 242 32 32 15
HincII GTy'rAC
Cuts at: 0 1011 1736 1800
Size: 1011 725 64
Fragments arranged by size:
1011 725 64
HindIII A'AGCT_T
Cuts at: 0 1268 1289 1556 1800
Size: 1268 21 267 244
Fragments arranged by size:
1268 267 244 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 488 1800
Size: 228 260 1312
Fragments arranged by size:
1312 260 228
MscI TGG'CCA
Cuts at: 0 283 418 475 1088 1800
Size: 283 135 57 613 712
Fragments arranged by size:
712 613 283 135 57
MslI CAynn'nnrTG
Cuts at: 0 412 976 1800
Size: 412 564 824
Fragments arranged by size:
824 564 412
MspA1I CmG'CkG
Cuts at: 0 45 479 771 1201 1800
Size: 45 434 292 430 599
Fragments arranged by size:
599 434 430 292 45
NcoI C'CATG_G
Cuts at: 0 1121 1226 1800
Size: 1121 105 574
Fragments arranged by size:
1121 574 105
NdeI CA'TA_TG
Cuts at: 0 1281 1800
Size: 1281 519
NspI r_CATG'y
Cuts at: 0 10 444 1800
Size: 10 434 1356
Fragments arranged by size:
1356 434 10
PciI A'CATG_T
Cuts at: 0 440 1800
Size: 440 1360
PfoI T'CCnGG_A
Cuts at: 0 200 366 1493 1800
Size: 200 166 1127 307
Fragments arranged by size:
1127 307 200 166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 427 459 1800
Size: 427 32 1341
Fragments arranged by size:
1341 427 32
PpuMI rG'GwC_Cy
Cuts at: 0 300 1800
Size: 300 1500
PspXI vC'TCGA_Gb
Cuts at: 0 1355 1800
Size: 1355 445
PstI C_TGCA'G
Cuts at: 0 153 1175 1800
Size: 153 1022 625
Fragments arranged by size:
1022 625 153
PvuII CAG'CTG
Cuts at: 0 479 771 1800
Size: 479 292 1029
Fragments arranged by size:
1029 479 292
SbfI CC_TGCA'GG
Cuts at: 0 1175 1800
Size: 1175 625
SexAI A'CCwGG_T
Cuts at: 0 632 809 1800
Size: 632 177 991
Fragments arranged by size:
991 632 177
SfcI C'TryA_G
Cuts at: 0 149 1171 1800
Size: 149 1022 629
Fragments arranged by size:
1022 629 149
SgrAI Cr'CCGG_yG
Cuts at: 0 99 1800
Size: 99 1701
SmaI CCC'GGG
Cuts at: 0 988 1800
Size: 988 812
SmlI C'TyrA_G
Cuts at: 0 272 1219 1355 1690 1800
Size: 272 947 136 335 110
Fragments arranged by size:
947 335 272 136 110
StuI AGG'CCT
Cuts at: 0 596 1800
Size: 596 1204
StyI C'CwwG_G
Cuts at: 0 1121 1226 1800
Size: 1121 105 574
Fragments arranged by size:
1121 574 105
Tth111I GACn'n_nGTC
Cuts at: 0 298 1800
Size: 298 1502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 1800
Size: 276 1524
XhoI C'TCGA_G
Cuts at: 0 272 1355 1800
Size: 272 1083 445
Fragments arranged by size:
1083 445 272
XmaI C'CCGG_G
Cuts at: 0 986 1800
Size: 986 814
XmnI GAAnn'nnTTC
Cuts at: 0 209 322 1658 1800
Size: 209 113 1336 142
Fragments arranged by size:
1336 209 142 113
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AleI AloI AlwNI
ApaLI ApoI AvaI BanII BbsI BbvCI BcgI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII
BstXI BstYI BtgI BtgZI BtsI DraI EaeI EarI
Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NcoI NdeI NspI PciI PfoI
PpiI PpuMI PspXI PstI PvuII SbfI SexAI SfcI
SgrAI SmaI SmlI StuI StyI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AlfI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BciVI BclI BfrBI BlpI BmgBI BmtI BsaAI BsaBI
BsiWI BsmBI BspHI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SfiI SfoI SgfI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI TstI
XbaI ZraI