Restriction map for
pFN21AE5769
(Linear) (Six-base) MAPSORT of: KIEE5769.seq Check: 635 from: 1 to: 1761
KIEE5769.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1341 1761
Size: 1341 420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1593 1761
Size: 956 637 168
Fragments arranged by size:
956 637 168
AflIII A'CryG_T
Cuts at: 0 950 1761
Size: 950 811
AlwNI CAG_nnn'CTG
Cuts at: 0 384 729 777 1761
Size: 384 345 48 984
Fragments arranged by size:
984 384 345 48
ApaI G_GGCC'C
Cuts at: 0 405 1761
Size: 405 1356
ApaLI G'TGCA_C
Cuts at: 0 374 1761
Size: 374 1387
AvaI C'yCGr_G
Cuts at: 0 5 326 354 421 793 1046 1307 1761
Size: 5 321 28 67 372 253 261 454
Fragments arranged by size:
454 372 321 261 253 67 28 5
BanI G'GyrC_C
Cuts at: 0 336 417 816 865 1318 1416 1761
Size: 336 81 399 49 453 98 345
Fragments arranged by size:
453 399 345 336 98 81 49
BanII G_rGCy'C
Cuts at: 0 196 405 431 1283 1365 1484 1604 1761
Size: 196 209 26 852 82 119 120 157
Fragments arranged by size:
852 209 196 157 120 119 82 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 693 1761
Size: 693 1068
BbvCI CC'TCA_GC
Cuts at: 0 538 1761
Size: 538 1223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 219 253 1761
Size: 219 34 1508
Fragments arranged by size:
1508 219 34
BclI T'GATC_A
Cuts at: 0 1430 1761
Size: 1430 331
BglI GCCn_nnn'nGGC
Cuts at: 0 461 1761
Size: 461 1300
BlpI GC'TnA_GC
Cuts at: 0 1117 1761
Size: 1117 644
Bme1580I G_kGCm'C
Cuts at: 0 378 397 405 422 821 1421 1761
Size: 378 19 8 17 399 600 340
Fragments arranged by size:
600 399 378 340 19 17 8
BmgBI CAC'GTC
Cuts at: 0 1163 1761
Size: 1163 598
BmrI ACTGGGnnnn_n'
Cuts at: 0 399 400 1378 1761
Size: 399 1 978 383
Fragments arranged by size:
978 399 383 1
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 196 1761
Size: 196 1565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 691 832 1018 1761
Size: 166 525 141 186 743
Fragments arranged by size:
743 525 186 166 141
Bpu10I CC'TnA_GC
Cuts at: 0 538 1761
Size: 538 1223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1761
Size: 961 800
BsaI GGTCTCn'nnnn_
Cuts at: 0 667 1057 1761
Size: 667 390 704
Fragments arranged by size:
704 667 390
BsaHI Gr'CG_yC
Cuts at: 0 583 1761
Size: 583 1178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1226 1256 1337 1367 1761
Size: 1226 30 81 30 394
Fragments arranged by size:
1226 394 81 30 30
BseYI C'CCAG_C
Cuts at: 0 39 717 1005 1056 1073 1179 1761
Size: 39 678 288 51 17 106 582
Fragments arranged by size:
678 582 288 106 51 39 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 535 638 1642 1761
Size: 213 322 103 1004 119
Fragments arranged by size:
1004 322 213 119 103
BsiHKAI G_wGCw'C
Cuts at: 0 378 431 1283 1365 1484 1761
Size: 378 53 852 82 119 277
Fragments arranged by size:
852 378 277 119 82 53
BsmI GAATG_Cn'
Cuts at: 0 1044 1761
Size: 1044 717
BsmBI CGTCTCn'nnnn_
Cuts at: 0 325 964 1728 1761
Size: 325 639 764 33
Fragments arranged by size:
764 639 325 33
Bsp1286I G_dGCh'C
Cuts at: 0 196 378 397 405 422 431 821 1283
Size: 196 182 19 8 17 9 390 462
Cuts at: 1283 1365 1421 1484 1604 1761
Size: 82 56 63 120 157
Fragments arranged by size:
462 390 196 182 157 120 82 63
56 19 17 9 8
BspHI T'CATG_A
Cuts at: 0 1511 1761
Size: 1511 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1061 1341 1761
Size: 1061 280 420
Fragments arranged by size:
1061 420 280
BsrBI CCG'CTC
Cuts at: 0 1546 1761
Size: 1546 215
BsrDI GCAATG_nn'
Cuts at: 0 740 1550 1761
Size: 740 810 211
Fragments arranged by size:
810 740 211
BsrFI r'CCGG_y
Cuts at: 0 339 1761
Size: 339 1422
BstAPI GCAn_nnn'nTGC
Cuts at: 0 865 1761
Size: 865 896
BstXI CCAn_nnnn'nTGG
Cuts at: 0 647 669 1642 1761
Size: 647 22 973 119
Fragments arranged by size:
973 647 119 22
Bsu36I CC'TnA_GG
Cuts at: 0 625 632 1732 1761
Size: 625 7 1100 29
Fragments arranged by size:
1100 625 29 7
BtgI C'CryG_G
Cuts at: 0 20 200 432 459 646 677 1149 1199
Size: 20 180 232 27 187 31 472 50
Cuts at: 1199 1252 1412 1641 1761
Size: 53 160 229 120
Fragments arranged by size:
472 232 229 187 180 160 120 53
50 31 27 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 198 483 1252 1314 1761
Size: 198 285 769 62 447
Fragments arranged by size:
769 447 285 198 62
BtsI GCAGTG_nn'
Cuts at: 0 1343 1761
Size: 1343 418
ClaI AT'CG_AT
Cuts at: 0 1094 1761
Size: 1094 667
DrdI GACnn_nn'nnGTC
Cuts at: 0 151 1161 1761
Size: 151 1010 600
Fragments arranged by size:
1010 600 151
EaeI y'GGCC_r
Cuts at: 0 341 462 659 680 779 926 1098 1133
Size: 341 121 197 21 99 147 172 35
Cuts at: 1133 1547 1644 1761
Size: 414 97 117
Fragments arranged by size:
414 341 197 172 147 121 117 99
97 35 21
EarI CTCTTCn'nnn_
Cuts at: 0 607 1732 1761
Size: 607 1125 29
Fragments arranged by size:
1125 607 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 281 1072 1761
Size: 281 791 689
Fragments arranged by size:
791 689 281
EcoICRI GAG'CTC
Cuts at: 0 429 1281 1363 1482 1761
Size: 429 852 82 119 279
Fragments arranged by size:
852 429 279 119 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 691 832 956 1018 1593 1761
Size: 166 525 141 124 62 575 168
Fragments arranged by size:
575 525 168 166 141 124 62
EcoO109I rG'GnC_Cy
Cuts at: 0 99 401 636 774 1011 1050 1664 1706
Size: 99 302 235 138 237 39 614 42
Cuts at: 1706 1761
Size: 55
Fragments arranged by size:
614 302 237 235 138 99 55 42
39
HaeII r_GCGC'y
Cuts at: 0 58 1199 1761
Size: 58 1141 562
Fragments arranged by size:
1141 562 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 161 193 260 292 1761
Size: 161 32 67 32 1469
Fragments arranged by size:
1469 161 67 32 32
HincII GTy'rAC
Cuts at: 0 243 587 1575 1761
Size: 243 344 988 186
Fragments arranged by size:
988 344 243 186
MscI TGG'CCA
Cuts at: 0 661 682 781 928 1646 1761
Size: 661 21 99 147 718 115
Fragments arranged by size:
718 661 147 115 99 21
MslI CAynn'nnrTG
Cuts at: 0 645 1761
Size: 645 1116
MspA1I CmG'CkG
Cuts at: 0 483 762 1761
Size: 483 279 999
Fragments arranged by size:
999 483 279
NaeI GCC'GGC
Cuts at: 0 341 1761
Size: 341 1420
NcoI C'CATG_G
Cuts at: 0 459 646 1412 1761
Size: 459 187 766 349
Fragments arranged by size:
766 459 349 187
NgoMIV G'CCGG_C
Cuts at: 0 339 1761
Size: 339 1422
NspI r_CATG'y
Cuts at: 0 523 1573 1761
Size: 523 1050 188
Fragments arranged by size:
1050 523 188
PasI CC'CwG_GG
Cuts at: 0 473 822 1443 1761
Size: 473 349 621 318
Fragments arranged by size:
621 473 349 318
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 1761
Size: 729 1032
PfoI T'CCnGG_A
Cuts at: 0 1283 1365 1761
Size: 1283 82 396
Fragments arranged by size:
1283 396 82
PpuMI rG'GwC_Cy
Cuts at: 0 636 774 1011 1050 1761
Size: 636 138 237 39 711
Fragments arranged by size:
711 636 237 138 39
PspOMI G'GGCC_C
Cuts at: 0 401 1761
Size: 401 1360
PstI C_TGCA'G
Cuts at: 0 773 861 1338 1761
Size: 773 88 477 423
Fragments arranged by size:
773 477 423 88
PvuII CAG'CTG
Cuts at: 0 483 762 1761
Size: 483 279 999
Fragments arranged by size:
999 483 279
SacI G_AGCT'C
Cuts at: 0 431 1283 1365 1484 1761
Size: 431 852 82 119 277
Fragments arranged by size:
852 431 277 119 82
SanDI GG'GwC_CC
Cuts at: 0 1011 1761
Size: 1011 750
SapI GCTCTTCn'nnn_
Cuts at: 0 607 1761
Size: 607 1154
SbfI CC_TGCA'GG
Cuts at: 0 861 1761
Size: 861 900
SfcI C'TryA_G
Cuts at: 0 769 857 1125 1334 1761
Size: 769 88 268 209 427
Fragments arranged by size:
769 427 268 209 88
SmaI CCC'GGG
Cuts at: 0 7 328 423 1048 1309 1761
Size: 7 321 95 625 261 452
Fragments arranged by size:
625 452 321 261 95 7
SmlI C'TyrA_G
Cuts at: 0 976 1761
Size: 976 785
StuI AGG'CCT
Cuts at: 0 118 630 933 1141 1761
Size: 118 512 303 208 620
Fragments arranged by size:
620 512 303 208 118
StyI C'CwwG_G
Cuts at: 0 149 459 646 746 986 1412 1761
Size: 149 310 187 100 240 426 349
Fragments arranged by size:
426 349 310 240 187 149 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1345 1761
Size: 1345 416
TatI w'GTAC_w
Cuts at: 0 839 1761
Size: 839 922
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1226 1258 1337 1369 1761
Size: 1226 32 79 32 392
Fragments arranged by size:
1226 392 79 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 103 219 457 690 1093 1761
Size: 103 116 238 233 403 668
Fragments arranged by size:
668 403 238 233 116 103
XmaI C'CCGG_G
Cuts at: 0 5 326 421 1046 1307 1761
Size: 5 321 95 625 261 454
Fragments arranged by size:
625 454 321 261 95 5
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BbvCI BcgI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BstAPI BstXI Bsu36I BtgI
BtgZI BtsI ClaI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SbfI SfcI
SmaI SmlI StuI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BfrBI BglII BmtI BsaAI
BsaBI BsaWI BseRI BsiEI BsiWI BspEI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I CspCI DraI DraIII
EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI ZraI