Restriction map for
pFN21AE5766
(Linear) (Six-base) MAPSORT of: KIEE5766.seq Check: 9955 from: 1 to: 1767
KIEE5766.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AatII G_ACGT'C
Cuts at: 0 200 1767
Size: 200 1567
AccI GT'mk_AC
Cuts at: 0 574 1767
Size: 574 1193
Acc65I G'GTAC_C
Cuts at: 0 478 1767
Size: 478 1289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 409 518 881 1250 1396 1767
Size: 235 174 109 363 369 146 371
Fragments arranged by size:
371 369 363 235 174 146 109
AhdI GACnn_n'nnGTC
Cuts at: 0 253 498 1767
Size: 253 245 1269
Fragments arranged by size:
1269 253 245
AlwNI CAG_nnn'CTG
Cuts at: 0 1219 1767
Size: 1219 548
ApaI G_GGCC'C
Cuts at: 0 508 1767
Size: 508 1259
ApoI r'AATT_y
Cuts at: 0 67 88 544 1336 1711 1767
Size: 67 21 456 792 375 56
Fragments arranged by size:
792 456 375 67 56 21
AvaI C'yCGr_G
Cuts at: 0 1633 1767
Size: 1633 134
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 640 673 1767
Size: 640 33 1094
Fragments arranged by size:
1094 640 33
BanI G'GyrC_C
Cuts at: 0 478 1767
Size: 478 1289
BanII G_rGCy'C
Cuts at: 0 508 1767
Size: 508 1259
BbsI GAAGACnn'nnnn_
Cuts at: 0 249 1384 1589 1767
Size: 249 1135 205 178
Fragments arranged by size:
1135 249 205 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 666 700 1767
Size: 666 34 1067
Fragments arranged by size:
1067 666 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 668 1767
Size: 668 1099
BclI T'GATC_A
Cuts at: 0 1601 1767
Size: 1601 166
BfrBI ATG'CAT
Cuts at: 0 1081 1767
Size: 1081 686
Bme1580I G_kGCm'C
Cuts at: 0 508 1767
Size: 508 1259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 263 953 1767
Size: 118 145 690 814
Fragments arranged by size:
814 690 145 118
BsaBI GATnn'nnATC
Cuts at: 0 1031 1048 1767
Size: 1031 17 719
Fragments arranged by size:
1031 719 17
BsaHI Gr'CG_yC
Cuts at: 0 197 1767
Size: 197 1570
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1725 1767
Size: 1725 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1664 1767
Size: 1664 103
BsiEI CG_ry'CG
Cuts at: 0 851 1767
Size: 851 916
BsiHKAI G_wGCw'C
Cuts at: 0 465 1255 1767
Size: 465 790 512
Fragments arranged by size:
790 512 465
BsmI GAATG_Cn'
Cuts at: 0 766 1767
Size: 766 1001
Bsp1286I G_dGCh'C
Cuts at: 0 465 508 1255 1767
Size: 465 43 747 512
Fragments arranged by size:
747 512 465 43
BsrDI GCAATG_nn'
Cuts at: 0 684 1767
Size: 684 1083
BstAPI GCAn_nnn'nTGC
Cuts at: 0 469 1108 1767
Size: 469 639 659
Fragments arranged by size:
659 639 469
BstZ17I GTA'TAC
Cuts at: 0 575 1767
Size: 575 1192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1653 1767
Size: 1653 114
ClaI AT'CG_AT
Cuts at: 0 402 1767
Size: 402 1365
DraI TTT'AAA
Cuts at: 0 1007 1767
Size: 1007 760
EarI CTCTTCn'nnn_
Cuts at: 0 37 329 1767
Size: 37 292 1438
Fragments arranged by size:
1438 292 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 235 263 409 518 881 953 1250
Size: 118 117 28 146 109 363 72 297
Cuts at: 1250 1396 1767
Size: 146 371
Fragments arranged by size:
371 363 297 146 146 118 117 109
72 28
EcoO109I rG'GnC_Cy
Cuts at: 0 1618 1686 1767
Size: 1618 68 81
Fragments arranged by size:
1618 81 68
EcoRV GAT'ATC
Cuts at: 0 540 1290 1767
Size: 540 750 477
Fragments arranged by size:
750 540 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 203 235 239 261 271 293 484 516
Size: 203 32 4 22 10 22 191 32
Cuts at: 516 597 629 1586 1618 1767
Size: 81 32 957 32 149
Fragments arranged by size:
957 203 191 149 81 32 32 32
32 22 22 10 4
HindIII A'AGCT_T
Cuts at: 0 1678 1767
Size: 1678 89
KpnI G_GTAC'C
Cuts at: 0 482 1767
Size: 482 1285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 1081 1224 1734 1767
Size: 933 148 143 510 33
Fragments arranged by size:
933 510 148 143 33
MslI CAynn'nnrTG
Cuts at: 0 1082 1132 1490 1767
Size: 1082 50 358 277
Fragments arranged by size:
1082 358 277 50
MspA1I CmG'CkG
Cuts at: 0 1219 1333 1767
Size: 1219 114 434
Fragments arranged by size:
1219 434 114
NsiI A_TGCA'T
Cuts at: 0 1083 1767
Size: 1083 684
NspI r_CATG'y
Cuts at: 0 1085 1767
Size: 1085 682
PasI CC'CwG_GG
Cuts at: 0 42 1767
Size: 42 1725
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 489 1767
Size: 42 447 1278
Fragments arranged by size:
1278 447 42
PpuMI rG'GwC_Cy
Cuts at: 0 1686 1767
Size: 1686 81
PspOMI G'GGCC_C
Cuts at: 0 504 1767
Size: 504 1263
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 356 388 762 794 1767
Size: 356 32 374 32 973
Fragments arranged by size:
973 374 356 32 32
PvuII CAG'CTG
Cuts at: 0 1333 1767
Size: 1333 434
SapI GCTCTTCn'nnn_
Cuts at: 0 37 1767
Size: 37 1730
SfcI C'TryA_G
Cuts at: 0 1479 1767
Size: 1479 288
SmaI CCC'GGG
Cuts at: 0 1635 1767
Size: 1635 132
SspI AAT'ATT
Cuts at: 0 447 750 829 1767
Size: 447 303 79 938
Fragments arranged by size:
938 447 303 79
StyI C'CwwG_G
Cuts at: 0 566 1767
Size: 566 1201
XmaI C'CCGG_G
Cuts at: 0 1633 1767
Size: 1633 134
ZraI GAC'GTC
Cuts at: 0 198 1767
Size: 198 1569
Enzymes that do cut:
AatII AccI Acc65I AcuI AhdI AlwNI ApaI ApoI
AvaI BaeI BanI BanII BbsI BcgI BciVI BclI
BfrBI Bme1580I BpmI BsaBI BsaHI BseRI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BsrDI BstAPI BstZ17I BtgZI ClaI
DraI EarI Eco57MI EcoO109I EcoRV Hin4I HindIII KpnI
MmeI MslI MspA1I NsiI NspI PasI PflMI PpuMI
PspOMI PsrI PvuII SapI SfcI SmaI SspI StyI
XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BbvCI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseYI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HaeII HincII HpaI
KasI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI