Restriction map for
pFN21AE5765
(Linear) (Six-base) MAPSORT of: KIEE5765.seq Check: 3286 from: 1 to: 2949
KIEE5765.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AccI GT'mk_AC
Cuts at: 0 1993 2440 2949
Size: 1993 447 509
Fragments arranged by size:
1993 509 447
Acc65I G'GTAC_C
Cuts at: 0 711 2949
Size: 711 2238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 298 1325 1730 1955 2110 2249 2762
Size: 166 132 1027 405 225 155 139 513
Cuts at: 2762 2949
Size: 187
Fragments arranged by size:
1027 513 405 225 187 166 155 139
132
AflIII A'CryG_T
Cuts at: 0 920 2949
Size: 920 2029
AgeI A'CCGG_T
Cuts at: 0 500 2949
Size: 500 2449
AlwNI CAG_nnn'CTG
Cuts at: 0 2740 2949
Size: 2740 209
ApoI r'AATT_y
Cuts at: 0 286 817 1533 2156 2501 2860 2949
Size: 286 531 716 623 345 359 89
Fragments arranged by size:
716 623 531 359 345 286 89
AvaI C'yCGr_G
Cuts at: 0 61 2949
Size: 61 2888
BanI G'GyrC_C
Cuts at: 0 711 1714 2949
Size: 711 1003 1235
Fragments arranged by size:
1235 1003 711
BanII G_rGCy'C
Cuts at: 0 68 2766 2949
Size: 68 2698 183
Fragments arranged by size:
2698 183 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 519 833 1519 2317 2949
Size: 519 314 686 798 632
Fragments arranged by size:
798 686 632 519 314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 42 76 2949
Size: 42 34 2873
Fragments arranged by size:
2873 42 34
BclI T'GATC_A
Cuts at: 0 14 162 933 2949
Size: 14 148 771 2016
Fragments arranged by size:
2016 771 148 14
BglII A'GATC_T
Cuts at: 0 2640 2781 2949
Size: 2640 141 168
Fragments arranged by size:
2640 168 141
BlpI GC'TnA_GC
Cuts at: 0 49 2949
Size: 49 2900
Bme1580I G_kGCm'C
Cuts at: 0 1719 2949
Size: 1719 1230
BmrI ACTGGGnnnn_n'
Cuts at: 0 381 1081 1109 2949
Size: 381 700 28 1840
Fragments arranged by size:
1840 700 381 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 683 1508 1517 1604 2224 2429 2949
Size: 236 447 825 9 87 620 205 520
Fragments arranged by size:
825 620 520 447 236 205 87 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 2949
Size: 22 2927
BsaI GGTCTCn'nnnn_
Cuts at: 0 491 2949
Size: 491 2458
BsaAI yAC'GTr
Cuts at: 0 852 2949
Size: 852 2097
BsaBI GATnn'nnATC
Cuts at: 0 80 464 968 2949
Size: 80 384 504 1981
Fragments arranged by size:
1981 504 384 80
BsaWI w'CCGG_w
Cuts at: 0 500 2949
Size: 500 2449
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 516 534 546 564 1103 1133 1830 1860
Size: 516 18 12 18 539 30 697 30
Cuts at: 1860 2402 2432 2949
Size: 542 30 517
Fragments arranged by size:
697 542 539 517 516 30 30 30
18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 239 467 929 1016 1172 1235 2949
Size: 239 228 462 87 156 63 1714
Fragments arranged by size:
1714 462 239 228 156 87 63
BseYI C'CCAG_C
Cuts at: 0 993 1917 2949
Size: 993 924 1032
Fragments arranged by size:
1032 993 924
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2030 2543 2949
Size: 2030 513 406
Fragments arranged by size:
2030 513 406
BsiHKAI G_wGCw'C
Cuts at: 0 2766 2949
Size: 2766 183
BsmI GAATG_Cn'
Cuts at: 0 1229 2949
Size: 1229 1720
Bsp1286I G_dGCh'C
Cuts at: 0 68 1719 2766 2949
Size: 68 1651 1047 183
Fragments arranged by size:
1651 1047 183 68
BspHI T'CATG_A
Cuts at: 0 822 2949
Size: 822 2127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1956 2949
Size: 1956 993
BsrBI CCG'CTC
Cuts at: 0 1914 2949
Size: 1914 1035
BsrDI GCAATG_nn'
Cuts at: 0 1105 2949
Size: 1105 1844
BsrFI r'CCGG_y
Cuts at: 0 500 2949
Size: 500 2449
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2604 2949
Size: 2604 345
BstYI r'GATC_y
Cuts at: 0 81 639 675 2640 2781 2949
Size: 81 558 36 1965 141 168
Fragments arranged by size:
1965 558 168 141 81 36
BstZ17I GTA'TAC
Cuts at: 0 2441 2949
Size: 2441 508
Bsu36I CC'TnA_GG
Cuts at: 0 1982 2949
Size: 1982 967
DraIII CAC_nnn'GTG
Cuts at: 0 1353 2949
Size: 1353 1596
EaeI y'GGCC_r
Cuts at: 0 729 2532 2949
Size: 729 1803 417
Fragments arranged by size:
1803 729 417
EarI CTCTTCn'nnn_
Cuts at: 0 317 335 477 711 1974 2864 2912 2949
Size: 317 18 142 234 1263 890 48 37
Fragments arranged by size:
1263 890 317 234 142 48 37 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 78 537 2949
Size: 78 459 2412
Fragments arranged by size:
2412 459 78
EcoICRI GAG'CTC
Cuts at: 0 2764 2949
Size: 2764 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 236 298 683 1325 1508 1517 1604
Size: 166 70 62 385 642 183 9 87
Cuts at: 1604 1730 1955 2110 2224 2249 2429 2762 2949
Size: 126 225 155 114 25 180 333 187
Fragments arranged by size:
642 385 333 225 187 183 180 166
155 126 114 87 70 62 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1130 1981 2949
Size: 1130 851 968
Fragments arranged by size:
1130 968 851
EcoO109I rG'GnC_Cy
Cuts at: 0 1082 2949
Size: 1082 1867
EcoRI G'AATT_C
Cuts at: 0 2501 2949
Size: 2501 448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 589 2662 2949
Size: 589 2073 287
Fragments arranged by size:
2073 589 287
FspI TGC'GCA
Cuts at: 0 2660 2949
Size: 2660 289
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 534 566 829 861 1447 1479
Size: 247 32 255 32 263 32 586 32
Cuts at: 1479 2402 2434 2683 2715 2914 2946 2949
Size: 923 32 249 32 199 32 3
Fragments arranged by size:
923 586 263 255 249 247 199 32
32 32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 0 1680 2949
Size: 1680 1269
HindIII A'AGCT_T
Cuts at: 0 1597 2949
Size: 1597 1352
HpaI GTT'AAC
Cuts at: 0 1680 2949
Size: 1680 1269
KpnI G_GTAC'C
Cuts at: 0 715 2949
Size: 715 2234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 2949
Size: 538 2411
MscI TGG'CCA
Cuts at: 0 731 2949
Size: 731 2218
MslI CAynn'nnrTG
Cuts at: 0 941 2949
Size: 941 2008
MspA1I CmG'CkG
Cuts at: 0 168 2032 2454 2749 2824 2949
Size: 168 1864 422 295 75 125
Fragments arranged by size:
1864 422 295 168 125 75
NdeI CA'TA_TG
Cuts at: 0 1281 2949
Size: 1281 1668
NspI r_CATG'y
Cuts at: 0 924 1360 2949
Size: 924 436 1589
Fragments arranged by size:
1589 924 436
PasI CC'CwG_GG
Cuts at: 0 2597 2949
Size: 2597 352
PciI A'CATG_T
Cuts at: 0 920 2949
Size: 920 2029
PfoI T'CCnGG_A
Cuts at: 0 1029 2313 2949
Size: 1029 1284 636
Fragments arranged by size:
1284 1029 636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2510 2542 2949
Size: 2510 32 407
Fragments arranged by size:
2510 407 32
PshAI GACnn'nnGTC
Cuts at: 0 923 2949
Size: 923 2026
PsiI TTA'TAA
Cuts at: 0 1697 2949
Size: 1697 1252
PstI C_TGCA'G
Cuts at: 0 1275 1953 2079 2754 2949
Size: 1275 678 126 675 195
Fragments arranged by size:
1275 678 675 195 126
PvuII CAG'CTG
Cuts at: 0 168 2032 2454 2749 2824 2949
Size: 168 1864 422 295 75 125
Fragments arranged by size:
1864 422 295 168 125 75
SacI G_AGCT'C
Cuts at: 0 2766 2949
Size: 2766 183
SapI GCTCTTCn'nnn_
Cuts at: 0 335 2949
Size: 335 2614
ScaI AGT'ACT
Cuts at: 0 1938 2949
Size: 1938 1011
SfcI C'TryA_G
Cuts at: 0 1214 1271 1949 2075 2750 2949
Size: 1214 57 678 126 675 199
Fragments arranged by size:
1214 678 675 199 126 57
SmlI C'TyrA_G
Cuts at: 0 37 61 2949
Size: 37 24 2888
Fragments arranged by size:
2888 37 24
SnaBI TAC'GTA
Cuts at: 0 852 2949
Size: 852 2097
SphI G_CATG'C
Cuts at: 0 1360 2949
Size: 1360 1589
SspI AAT'ATT
Cuts at: 0 2277 2817 2949
Size: 2277 540 132
Fragments arranged by size:
2277 540 132
StyI C'CwwG_G
Cuts at: 0 1673 2949
Size: 1673 1276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 876 1128 1425 1840 2949
Size: 876 252 297 415 1109
Fragments arranged by size:
1109 876 415 297 252
TatI w'GTAC_w
Cuts at: 0 1936 2949
Size: 1936 1013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1103 1135 2949
Size: 1103 32 1814
Fragments arranged by size:
1814 1103 32
Tth111I GACn'n_nGTC
Cuts at: 0 2321 2949
Size: 2321 628
XbaI T'CTAG_A
Cuts at: 0 361 2949
Size: 361 2588
XhoI C'TCGA_G
Cuts at: 0 61 2949
Size: 61 2888
XmnI GAAnn'nnTTC
Cuts at: 0 290 486 2505 2642 2861 2949
Size: 290 196 2019 137 219 88
Fragments arranged by size:
2019 290 219 196 137 88
Enzymes that do cut:
AccI Acc65I AcuI AflIII AgeI AlwNI ApoI AvaI
BanI BanII BbsI BcgI BclI BglII BlpI Bme1580I
BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstXI BstYI BstZ17I Bsu36I DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI Hin4I HincII HindIII HpaI KpnI MmeI
MscI MslI MspA1I NdeI NspI PasI PciI PfoI
PpiI PshAI PsiI PstI PvuII SacI SapI ScaI
SfcI SmlI SnaBI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BfrBI BglI BmgBI BmtI
BplI Bpu10I BsaHI BsiEI BsiWI BsmBI BspEI BsrGI
BssHII BssSI BstAPI BstBI BstEII BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EagI EcoRV FseI FspAI
HaeII KasI MfeI MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpuMI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SpeI SrfI StuI SwaI XcmI XmaI ZraI